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Use of biological selenium and glutathion as fish feedstuff additive

A technology of glutathione and biological selenium, which is applied in animal feed, animal feed, application, etc., can solve the problems that cannot be removed well, threaten human health, etc., and achieve the goal of reducing post-processing costs, enhancing capabilities, and ensuring safety Effect

Inactive Publication Date: 2009-05-27
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] The object of the present invention is to provide a kind of biological selenium and toxins for aquatic products, especially cultured fish body toxins, especially algae toxins, which are seriously enriched and the existing technology cannot remove these toxins well, thus causing serious threats to human health. The application of glutathione as a fish feed additive, fish bait additives containing bio-selenium and glutathione can promote the expression of fish detoxification genes, without any special treatment of cultivated aquatic products, and no need Any special processing device, the fish itself can excrete the ingested toxins, thus ensuring the safety of edible fish

Method used

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  • Use of biological selenium and glutathion as fish feedstuff additive
  • Use of biological selenium and glutathion as fish feedstuff additive
  • Use of biological selenium and glutathion as fish feedstuff additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Fish feed containing biological selenium and glutathione

[0044] The preparation method of the fish feed containing biological selenium and glutathione is as follows:

[0045] (1) Get the selenium-enriched yeast purchased from Guangzhou Shenggewang Biotechnology Co., Ltd., its content component is (mass percentage)

[0046] Moisture ≤10.0%

[0047] Protein (as N) ≥40.0%

[0048] Ash ≤10.0%

[0049] Selenium (as Se) 300-2000mg / kg

[0050] (2) Take glutathione purchased from AMRESCO, the purity of the glutathione is >98.0%:

[0051] (3) According to the amount of about 0.5 grams of selenium-enriched yeast per kilogram of dry feed and about 0.8 grams of glutathione per kilogram of dry feed, selenium-enriched yeast and glutathione were mixed and purchased from Guangzhou Aoyang Industry Limited fish feed that is available.

Embodiment 2

[0052] Example 2 Fish feed containing biological selenium and glutathione

[0053] The preparation method of the fish feed containing biological selenium and glutathione is as follows:

[0054] (1) Take selenium-enriched yeast purchased from Guangzhou Shenggewang Biotechnology Co., Ltd., and its content components are (mass percentage):

[0055] Moisture ≤10.0%

[0056] Protein (as N) ≥40.0%

[0057] Ash ≤10.0%

[0058] Selenium (as Se) 300-2000mg / kg

[0059] (2) Take glutathione purchased from AMRESCO, the purity of the glutathione is >98.0%:

[0060] (3) According to the amount of about 1.0 grams of selenium-enriched yeast per kilogram of dry feed and about 1.2 grams of glutathione per kilogram of dry feed, the selenium-rich yeast and glutathione were mixed and purchased from Guangzhou Aoyang Industry Limited fish feed that is available.

Embodiment 3

[0061] Example 3 Fish feed containing biological selenium and glutathione promotes the detection of fish detoxification gene expression

[0062] In this embodiment, the mRNA relative levels of microcystin detoxification enzyme genes in the liver and muscle microcystin detoxification enzyme genes of different species and strains were compared with the semi-quantitative PCR method, and β-actin was used as an external reference (we have cloned Tilapia non-fish β-actin cDNA sequence) to compare the microcystin detoxification ability of liver and muscle of different species and strains of tilapia. For specific methods, see literature: Liang, X.F., Ogata, H.Y., Oku, H., Chen, J., Hwang, F., 2003. Abundant and constant expression of uncoupling protein 2 in the liverer of red Sea bream Pagrus major. Comparative Biochemistry and Physiology A136: 655-661.

[0063]In this example, according to the method of Liang et al. (2002), the amount of microcystin detoxification enzyme gene mRNA i...

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Abstract

The invention discloses an application of biological selenium and glutathione as fish feed additives, wherein, the using amount of the biological selenium is that each kilogram of dry powder feed contains 0.5-1.0g of selenium-enriched yeast, and the using amount of the glutathione is that each kilogram of the dry powder feed contains 0.8-1.2g of the glutathione. The fish feed which is prepared by adding the biological selenium and the glutathione according to the application can promote the expression of detoxification genes of fish, especially the expression of I and II time phase metabolism detoxification-related genes, the fish feed can not only enhance the ability of resisting the outside poisons of the fish, but also be conductive to the decomposition and the removal of microcystins and drug toxins of the fish by the self-metabolic system, thereby ensuring the safety of eating the fish; the fish feed does not need to carry out the special treatment on the cultured aquatic products and does not need any special processing device, thereby greatly reducing the post-processing costs of the edible fish and being more environment-friendly and economic.

Description

technical field [0001] The invention relates to the field of fish bait additives, in particular to the application of biological selenium and glutathione as fish bait additives. Background technique [0002] In marine aquaculture, domoic acid (DA), diarrheal shellfish poisoning, paralytic shellfish poisoning, neurological shellfish poisoning in red tide toxins are stored and accumulated in shellfish differently, and they are very harmful. Domoic acid is more harmful and can be enriched in fish and shellfish, which belongs to amnesia shellfish poisoning. So far, DA has been detected all over the world, and the resulting poisoning incidents of humans and animals emerge in endlessly. In addition, the potential threat of malachite green in the prohibited fishery medicine to the quality and safety of marine aquaculture aquatic products in my country is particularly serious, and has caused major economic losses many times. Malachite green can bind or insert DNA, promote tumor gro...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/18A23K10/16A23K20/142A23K50/80
Inventor 梁旭方王琳陈小佳李光照程伟轩李观贵林群刘秀霞何珊胡永乐
Owner JINAN UNIVERSITY
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