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Application of tert butyl hydroquinone in bait additive for activating expression of fish detoxication genes

A technology of tert-butyl hydroquinone and gene expression, which is applied in the field of fish farming and detoxification treatment of farmed fish, can solve the problems that have not yet been reported on tert-butyl hydroquinone, and achieve enhanced resistance The ability of external poisons, reducing the cost of post-processing, and ensuring the effect of safety

Inactive Publication Date: 2010-09-01
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no relevant research report on the use of tert-butylhydroquinone as a fish feed additive

Method used

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  • Application of tert butyl hydroquinone in bait additive for activating expression of fish detoxication genes
  • Application of tert butyl hydroquinone in bait additive for activating expression of fish detoxication genes
  • Application of tert butyl hydroquinone in bait additive for activating expression of fish detoxication genes

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1 contains the fish feed of tBHQ

[0036] Take tBHQ purchased from SIGMA, and add tBHQ to the fish feed purchased from Guangzhou Aoyang Industrial Co., Ltd. to prepare tBHQ-containing fish feed according to the amount of about 0.1 g of tBHQ per kg of dry feed.

Embodiment 2

[0037] Example 2 Induction detection of tBHQ on the transcription level of fish liver detoxification-related genes

[0038]In this example, the in vitro cell culture induction method was used to cultivate the isolated liver cells of Nile tilapia and red sea bream, and the real-time fluorescent quantitative PCR method was used to detect the relative level of mRNA of the detoxification enzyme gene after hepatocyte induction and culture, and expressed as β- Actin was used as an external reference (we have cloned tilapia and red sea bream β-actin cDNA sequences), and compared the induced expression of detoxification enzyme genes in isolated liver cells after tBHQ induction. After gavage of Nile tilapia and red sea bream with toxin-containing and tBHQ-containing feed, real-time fluorescent quantitative PCR method was used to detect the relative levels of liver detoxification enzyme gene mRNA in Nile tilapia and red sea bream, and β-actin The protein was used as an external referenc...

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Abstract

The invention discloses application of tert butyl hydroquinone in a bait additive for activate expression of fish detoxication genes. In the invention, tert butyl hydroquinone is added to fish bait; serving as the expression inductive agent of a GST gene in a fish body, the tert butyl hydroquinone can induce fish GST gene expression, thus enabling fish to enhance ability to detoxicate and achieving the purpose of improving safety of aquatic products. When the tert butyl hydroquinone is served as the bait additive and is added into the fish bait, the adding amount is 0.05-0.15g in per kilogram of dry feed.

Description

technical field [0001] The invention relates to the technical field of fish farming, in particular to the technical field of detoxification treatment of farmed fish, and in particular to the application of tert-butylhydroquinone as a bait additive for activating the expression of fish detoxification genes. Background technique [0002] Pollution of toxic substances such as red tide toxins and prohibited fish medicines in seawater fish is a key issue in the quality and safety of marine aquaculture aquatic products in my country. For example, domoic acid in red tide toxins can be enriched in fish, resulting in poisoning of humans and animals Incidents emerge in endlessly; malachite green in illegal fish medicine can bind or insert DNA, promote tumor growth, and has obvious high carcinogenic, high teratogenic and mutagenic effects; in freshwater aquaculture, the toxin produced by Microcystis aeruginosa - Microcystis The toxin can cause damage to liver cells, and in severe cases, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/18
Inventor 梁旭方姚煜陈小佳王琳程伟轩李观贵何珊胡永乐郁颖陈亮
Owner JINAN UNIVERSITY
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