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Microfludic chip for rapid detection of microcystins and preparation method thereof

A technology of microcystin and microfluidic chip, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of large volume and not suitable for the environment of freshwater lakes

Inactive Publication Date: 2010-08-25
FUDAN UNIV
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  • Claims
  • Application Information

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Problems solved by technology

However, the equipment is bulky and not suitable for the environment of freshwater lakes

Method used

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  • Microfludic chip for rapid detection of microcystins and preparation method thereof
  • Microfludic chip for rapid detection of microcystins and preparation method thereof
  • Microfludic chip for rapid detection of microcystins and preparation method thereof

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Embodiment Construction

[0038] Substrate preparation. Put the silicon chip into Piranha solution (98% concentrated sulfuric acid: 30% hydrogen peroxide = 7: 3) and boil for 15 min. Rinse five times with deionized water, blow dry with nitrogen, and bake at 200 °C for 30 min.

[0039] Spray paint. Pour Microchem's SU-8 glue (the same below) on the center of the silicon wafer, hold the edge of the silicon wafer with your hand to tilt it and rotate it slowly, so that SU-8 covers most of the silicon wafer. Use a spin coater (Spin-Coater KW-4A, Chemat Technology, Inc.) to spin coat at 3000 rpm for 60s to make the glue more evenly distributed, and let it stand for 10min to alleviate the edge protrusion effect.

[0040] Soft bake. The purpose of soft baking is to volatilize the solvent in the SU-8 photoresist, and the key to process control is to volatilize the solvent at a controllable rate. Keep at 65°C, 95°C and 65°C for 3min, 6min and 3min, respectively. Then slowly lower to room temperature at a ra...

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Abstract

The invention belongs to the technical field of biological analysis and detection, in particular to a microfludic chip for rapid detection of microcystins and a preparation method thereof. The microfludic chip is made of optically transparent polydimethylsiloxane and the like by a molding method, and mainly comprises a sample reaction microchannel layer, a valve control layer and a substrate layer. The microfludic chip comprises a sample enriched and immunoassay module which consists of parallel immune chromatographic column microanalysis chambers in nanolitre volume. Microcystin antibody proteins or antigens are fixed in each analysis chamber, and rapid on-site detection of representative algal toxins, namely microcystins in samples with different sources is realized. Besides the advantages of high sensitivity and specificity, the microfludic chip has the characteristics of rapidness, high efficiency, portability, low cost and easily automatic control, can finish automatic signal acquisition, remote transmission and signal analysis, and is suitable for on-site rapid detection and remote control detection in a wide range for the microcystins in the water environment.

Description

technical field [0001] The invention belongs to the technical field of biological analysis and detection, and in particular relates to a microfluidic chip for on-site rapid detection of microcystins (microcystins), and provides a preparation method of the chip. Background technique [0002] "Red tides" and "algae blooms" in fresh water are a global problem. There have been "red tides" in major rivers and seas all over the world. At present, the occurrence frequency and scope of "red tides" and "water blooms" are becoming more and more serious. There have been reports of multiple outbreaks in my country's main bays and inland major lakes (Taihu Lake, Dianhu Lake, Chaohu Lake), which seriously affect the ecological environment, aquaculture and human health. Poisoning caused by algae toxins has also been reported many times. Florida, USA has a population of 8 million, and there were 500 related poisoning incidents reported from 1994 to 2005 alone. According to records, there ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N30/02
Inventor 隋国栋刘思秀张金玲赵望刘超
Owner FUDAN UNIV
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