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Method for improving efficiency of removing microcystin by probiotics

A microcystin and probiotic technology, applied in the field of food biology, can solve the problems of being unsuitable for removing microcystins, and the removal efficiency of microcystins is low, so as to solve the health hazards, improve the application effect, and reduce the content of microcystins. Effect

Inactive Publication Date: 2010-06-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the removal methods of microcystins mainly include chemical oxidation removal, physical adsorption method and biological method. The microorganisms used in the removal of algal toxins by biological methods are mainly concentrated in Sphingomonas, Pseudomonas aeruginosa and Delftia acidovorans , but none of the above strains are suitable for removing microcystins from food systems
[0004] Probiotics are important physiological bacteria in the human intestinal tract, which have physiological effects such as anti-tumor, alleviating lactose intolerance, enhancing immunity, lowering cholesterol, and adjusting intestinal flora. Algal toxins have achieved good results, but compared with other strains, the removal efficiency of probiotics to microcystins is still low, and there is an urgent need to improve the efficiency of probiotics to remove microcystins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Fermentation production of Lactobacillus salivarius:

[0023] Lactobacillus salivarius ATCC 29602 uses MRS medium: peptone 10g, beef extract 10g, yeast powder 5g, K 2 HPO 4 2g, diammonium citrate 2g, sodium acetate 5g, glucose 20g, Tween 80 1mL, MgSO 4 ·7H 2 O 0.58g, MnSO 4 4H 2 O 0.25g, distilled water 1000mL.

[0024] The cultivation method of Lactobacillus salivarius is as follows: get 2-3 rings of Lactobacillus salivarius from the well-growth flat plate and move it into a small centrifuge tube filled with 0.5ml sterile glycerol (glycerol concentration is 15%-20%), after covering Seal the mouth of the tube with parafilm and store in a -20°C refrigerator. The Lactobacillus salivarius preserved in the glycerol tube was inserted into the MRS liquid medium, and cultured statically at 37°C for 20 hours to the middle and late logarithmic stages.

[0025] Refining of Lactobacillus salivarius sludge:

[0026] Take Lactobacillus salivarius fermentation broth in the m...

Embodiment 2

[0031] Fermentation production of Lactobacillus salivarius:

[0032] Same as Example 1

[0033] Refining of Lactobacillus salivarius sludge:

[0034] Same as Example 1

[0035] Glucose improves the efficiency of Lactobacillus salivarius in removing algal toxins:

[0036] The count of Lactobacillus salivarius adopts the counting method of viable bacteria, the bacterial suspension is diluted to a certain number of times, spread on the plate, and the number of colonies is calculated.

[0037] will be 10 10 CFU / mL Lactobacillus fermentum was put into the water containing 1800μg / L microcystin (pH 7.0), added 10% (w / v) glucose, 37°C, 150rpm shaking culture for 24h, the clearance rate of microcystin 84.8%.

Embodiment 3

[0039] Fermentative production of Lactobacillus fermentum:

[0040] Lactobacillus fermentum (Lactobacillus fermentum ATCC 9338) uses MRS medium: peptone 10g, beef extract 10g, yeast powder 5g, K 2 HPO 4 2g, diammonium citrate 2g, sodium acetate 5g, glucose 20g, Tween 801mL, MgSO 4 ·7H 2 O 0.58g, MnSO 4 4H 2 O 0.25g, distilled water 1000mL.

[0041] The cultivation method of Lactobacillus fermentum is as follows: Get 2-3 rings of Lactobacillus fermentum from the well-growth plate and move it into a small centrifuge tube filled with 0.5ml sterile glycerin (glycerol concentration is 15%-20%), after covering Seal the mouth of the tube with parafilm and store in a -20°C refrigerator. The Lactobacillus fermentum preserved in the glycerol tube was inserted into the MRS liquid medium, and cultured statically at 37°C for 20h to the middle and late logarithmic stages.

[0042] Refining of Lactobacillus fermentum sludge:

[0043] The fermentation liquid of Lactobacillus fermentu...

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Abstract

The invention relates to a method for improving efficiency of removing microcystin by probiotics, which belongs to the technical field of food organisms. The fermentation liquor which is abundant in probiotics is prepared by adopting the probiotics as a biological catalyst through liquid deep fermentation or the fermentation liquor is refined to obtain bacterial mud; the obtained probiotic fermentation liquor or the bacterial mud is charged into water; meanwhile, glucose as an external additive is placed to carry out vibration culture at 37 DEG C for 24-30 hours by 150rpm, and the removing efficiency of the probiotics on the microcystin is obviously improved. Because the glucose and the probiotics have no harm to the safety of humans and livestock and have the health effect, the invention can be widely applied to the field of foods, and a healthy and safe product with rich nutrient components is obtained.

Description

technical field [0001] The invention discloses a method for improving the microcystin removal efficiency of probiotics, which belongs to the field of food biotechnology. Background technique [0002] Microcystins (MCs) are cyclic heptapeptide substances produced by cyanobacteria such as Microcystis aeruginosa, Anabaena blooms, and Nostoc algae in eutrophic water bodies. Microcystins target the liver of animals and can specifically inhibit the activity of protein phosphatases to induce a series of diseases such as cancer. Epidemiological surveys have shown that there is a great correlation between microcystin pollution in drinking water and the incidence of primary liver cancer in the population. [0003] At present, the removal methods of microcystins mainly include chemical oxidation removal, physical adsorption method and biological method. The microorganisms used in the removal of algal toxins by biological methods are mainly concentrated in Sphingomonas, Pseudomonas aer...

Claims

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Application Information

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IPC IPC(8): A23L1/015C12N1/20C12R1/225A23L5/20
Inventor 陈坚王松王淼堵国成张娟毕洁张茜
Owner JIANGNAN UNIV
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