Medetomidine hapten, monoclonal antibody, hybridoma cell strain and application thereof

By synthesizing metoprimidine hapten and complete antigen, a highly sensitive hybridoma cell line was prepared, solving the problems of expensive equipment and complex sample purification in existing metoprimidine detection technologies, and realizing a rapid and simple detection method.

CN121343922APending Publication Date: 2026-01-16JIANGNAN UNIV
View PDF 0 Cites 1 Cited by

Patent Information

Application Number
CN202511417785.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-09-30
Publication Date
2026-01-16

AI Technical Summary

Technical Problem

Existing methods for detecting tomididine in the United States require thorough sample purification, consume a lot of solvent, and have expensive equipment. They lack rapid and simple detection methods and also lack monoclonal antibodies with high specificity and sensitivity.

Method used

By synthesizing the medetomidine hapten, the medetomidine complete antigen was prepared. Hybridoma cell lines secreting medetomidine monoclonal antibodies were obtained by immunizing mice. High-sensitivity hybridoma cell lines were screened and applied to enzyme-linked immunosorbent assay (ELISA) detection.

Benefits of technology

A highly sensitive and specific medemidin monoclonal antibody was obtained, which can be effectively applied to rapid and convenient medemidin detection, suitable for on-site testing of large numbers of samples, and reduces testing costs.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN121343922A_ABST
    Figure CN121343922A_ABST
Patent Text Reader

Abstract

The invention relates to a medetomidine hapten, a monoclonal antibody, a hybridoma cell strain and application thereof, and belongs to the technical field of immunodetection. A medetomidine complete antigen is obtained by synthesizing a medetomidine hapten, a mouse is used as an immune animal to prepare a hybridoma cell strain secreting a medetomidine monoclonal antibody, the hybridoma cell strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.46518. The medetomidine monoclonal antibody secreted by the hybridoma cell strain has better detection sensitivity and detection specificity to medetomidine, the IC50 value is 10.42 ng / mL, and the cross reaction rate to various medetomidine analogues is lt; the method can be applied to preparation of medetomidine detection products, and provides a reliable tool for immunodetection of medetomidine.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Cited By

  • Metomidine haptens and complete antigens, and methods of making and using the same

    CN122355942A