Method for preparing docosahexenoic acid from schizochytrium sp.

A technology of docosahexaenoic acid and Schizochytrium, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve time-consuming and labor-consuming, serious secondary pollution, Schizochytrium Inability to grow and other problems, to achieve the effect of low cost, low cost, and few steps

Active Publication Date: 2016-02-24
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in addition to the carbon and nitrogen sources necessary for cell growth after cellulose hydrolysis, there are often by-products of lignin degradation such as formic acid, acetic acid, 5-hydroxymethylfurfural and other substances that are toxic to cells. Wild-type Schizochytrium cannot grow in cellulose hydrolyzate at all
The conventional solution to this problem is to detoxify the cellulose hydrolyzate to meet the growth requirements of Schizochytrium, but the detoxification process is time-consuming and labor-intensive, and the secondary pollution is serious

Method used

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  • Method for preparing docosahexenoic acid from schizochytrium sp.

Examples

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preparation example 1

[0045] The preparation method of bagasse hydrolyzate comprises the following steps:

[0046] (1) Take the bagasse after sugar extraction, place it in a blast drying box, and dry it for 3 days at 60°C until its water content is lower than 5wt%;

[0047] (2) pulverize the dried bagasse with a pulverizer, and sieve to obtain bagasse powder with a particle diameter of 2 to 20 mm;

[0048] (3) Mix bagasse powder with 0.01mol / L H 2 SO 4 The solution was mixed according to the solid-to-liquid ratio of 1:6, reacted at 120°C for 60 minutes, filtered, and the filtrate was Ca(OH) 2 Adjust the pH to 5, let stand for 1 hour, and filter to obtain bagasse hydrolysis stock solution.

preparation example 2

[0050] The preparation method of bagasse hydrolyzate comprises the following steps:

[0051] (1) Take the bagasse after sugar extraction, place it in a blast drying box, and dry it for 2 days at 80°C until its water content is lower than 5wt%;

[0052] (2) pulverize the dried bagasse with a pulverizer, and sieve to obtain bagasse powder with a particle diameter of 2 to 16 mm;

[0053] (3) Mix bagasse powder with 0.02mol / L H 2 SO 4 The solution was mixed according to the solid-to-liquid ratio of 1:3, reacted at 150°C for 120min, filtered, and the filtrate was Ca(OH) 2 Adjust the pH to 7, let stand for 1 hour, and filter to obtain bagasse hydrolysis stock solution.

preparation example 3

[0055] The preparation method of bagasse hydrolyzate comprises the following steps:

[0056] (1) Take the bagasse after sugar extraction, place it in a blast drying oven, and dry it for 2.5 days at 70°C until its water content is lower than 5wt%;

[0057] (2) pulverize the dried bagasse with a pulverizer, and sieve to obtain bagasse powder with a particle diameter of 2 to 15 mm;

[0058] (3) Mix bagasse powder with 0.01mol / L H 2 SO 4 The solution was mixed according to the solid-to-liquid ratio of 1:8, reacted at 140°C for 200min, filtered, and the filtrate was Ca(OH) 2 Adjust the pH to 6, let stand for 2 hours, and filter to obtain bagasse hydrolysis stock solution.

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Abstract

The invention discloses a method for preparing docosahexenoic acid from schizochytrium sp. The method comprises the following steps: (1) culturing a schizochytrium sp. strain capable of growing in a virus-infected bagasse hydrolysate directly, so as to obtain a seed fluid; (2) inoculating the seed fluid into a fermentation culture fluid prepared from the virus-infected bagasse hydrolysate for fermentation culture, so as to obtain docosahexenoic acid. The method is low in cost, easy to control, high in schizochytrium sp. yield and DHA content.

Description

technical field [0001] The invention relates to a method for producing docosahexaenoic acid, in particular to a method for producing docosahexaenoic acid by using Schizochytrium fungus. Background technique [0002] Docosahexaenoic acid (DHA, C22:6n-3) is an omega-3 fatty acid that is very beneficial to human health. Human cerebral cortex, retina, testis and sperm are particularly rich in DHA. DHA is one of the indispensable components of brain structural fatty acids. DHA has a very good effect on the treatment of atherosclerosis, hypertriglyceridemia, hypertension and cancer, and plays an important role in the development of infant intelligence and vision. DHA also has the ability to prevent coronary heart disease and reduce the risk of heart disease. Sudden death rate, prevention of senile dementia and anti-aging effects. Due to the lack of enzymes required for the synthesis of omega-3 fatty acids, human beings cannot synthesize DHA by themselves and can only be ingested...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
Inventor 张明亮祁峰黄建忠徐威江贤章孙磊
Owner FUJIAN NORMAL UNIV
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