Method for producing composite polyunsaturated fatty acid oil by using schizochytrium sp.

A technology of unsaturated fatty acids and Schizochytrium, which is applied in the field of production of complex polyunsaturated fatty acid oils, can solve problems such as the development of difficult-to-compound PUFAs products, and achieve significant industrial development value.

Pending Publication Date: 2019-08-09
INFINITUS (CHINA) CO LTD
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  • Summary
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AI Technical Summary

Problems solved by technology

[0004] At present, under ordinary fermentation conditions, the DHA content in Schizochytrium oil reaches more than 35%, while the EPA content is only about 0.5% to 1.0%. The EPA content is far lower than the EPA content in fish oil, and it is difficult to develop compound PUFAs products.

Method used

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Examples

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Embodiment 1

[0058] The method of utilizing Schizochytrium sp. to produce compound polyunsaturated fatty acid oil in the present embodiment is as follows:

[0059] (1) Preparation of fermentation medium (300L): glucose 30kg, sodium glutamate 6kg, yeast powder 1.2kg, magnesium sulfate 0.672kg, ammonium sulfate 1.44kg, KCl 0.3kg, KH 2 PO 4 1.05kg, Na 2 SO 4 11.1kg; inoculation fermentation, inoculum size 10%, rotation speed 300rpm, ventilation volume 25m 3 / h, temperature 26°C, pH natural. At 48 hours, 12kg of sugar was added at one time.

[0060] (2) The fermented liquid of Schizochytrium algae is centrifuged or plate-and-frame press-filtered to obtain wet cells, and water is added according to the ratio of algae mud to water at 1:2, and the cells of Schizochytrium algae are resuspended;

[0061] (3) add cellulase (food grade, 100,000 U / g, Henan Wanbang Industrial Co., Ltd.) and neutral protease (food grade, 200,000 U / g, Henan Wanbang Industrial Co., Ltd.) by 2500U / g (2: 8, U:U), the...

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Abstract

The invention relates to the field of microbial oils, in particular to a method for producing a composite polyunsaturated fatty acid oil by using schizochytrium sp.. The method comprises the steps that the schizochytrium sp. is subjected to fermentation culture; bacteria after fermentation culture are resuspended with water, and cellulase and neutral protease are added for enzymolysis; an enzymatic hydrolyzate is mixed with n-hexane, shaking extraction and centrifugation are conducted, an n-hexane phase is collected, concentration under reduced pressure is conducted to remove the n-hexane, anddrying is conducted to obtain an oil; the oil is subjected to first-stage crystallization by means of a freezing crystallization technique, the liquid oil is subjected to second-stage crystallization, and cold filtration is conducted to obtain the liquid oil after secondary crystallization; the liquid oil is deacidified and decolorized. The method has the advantages that by regulating fermentation conditions and conducting concentration processing on polyunsaturated fatty acids, the content of eicosapentaenoic acid in the schizochytrium sp. is successfully increased to 12% or above, and the produced oil is rich in docosahexaenoic acid, docosapentaenoic acid and eicosapentaenoic acid and is a novel oil product capable of replacing the polyunsaturated fatty acids from fish oils.

Description

technical field [0001] The invention relates to the field of microbial oils, in particular to a method for producing complex polyunsaturated fatty acid oils by utilizing Schizochytrium algae. Background technique [0002] Currently commercially available ω-3 polyunsaturated fatty acids (PUFAs) are mainly derived from marine fish. Fish oil as a source is seriously affected by fishy odor and stability, which limits its use as food additives and food accessories. Global fish resources have tended to decrease due to overfishing, and the increasingly scarce marine fish oil is difficult to meet people's growing demand for eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). In addition, the supply and quality of fish oil are greatly affected by seasonal and weather changes, and marine pollution caused by toxic impurities, heavy metals and organic matter also limits the further development of fish oil-derived PUFAs. The high market price of ω-3PUFAs makes many potential app...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/04C11B7/00
CPCC11B1/025C11B7/0075C12N1/12C12N1/06C12P7/6472C12N2500/05C12N2500/32C12N2500/34C12P7/6427C12P7/6434C12P7/6432
Inventor 陈则华周勇唐健王永华肖正荆琛峰梁东
Owner INFINITUS (CHINA) CO LTD
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