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Culture medium added with surfactant and application thereof

A surfactant and culture medium technology, applied in the field of microbial applications, can solve problems such as unreported research, achieve the effects of enhancing oxygen mass transfer, promoting growth, and reducing fermentation costs

Active Publication Date: 2014-07-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the use of surfactants in the supplementation of Schizochytrium culture medium

Method used

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  • Culture medium added with surfactant and application thereof
  • Culture medium added with surfactant and application thereof
  • Culture medium added with surfactant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: The effect of the amount of Tween 20 added on the fermentation of Schizochytrium

[0030] Schizochytrium seed medium: glycerol 30g / L, yeast extract 5g / L, sodium glutamate 5g / L, Na 2 SO 4 15g / L, KH 2 PO 4 3g / L, K 2 HPO 4 2g / L, MgSO 4 ·7H 2 O3g / L, K 2 SO 4 1g / L, VB 1 0.005g / L, VB 6 0.002g / L, VB 12 0.005g / L.

[0031] Schizochytrium fermentation medium: glycerol 100g / L, yeast extract 10g / L, sodium glutamate 15g / L, Na 2 SO 4 15g / L, KH 2 PO 4 3g / L, K 2 HPO 4 2g / L, MgSO 4 ·7H 2 O3g / L, K 2 SO 4 1g / L, VB 1 0.008g / L, VB 6 0.002g / L, VB 12 0.008g / L.

[0032] Then, Tween 20 was added to the fermentation medium of Schizochytrium, and the addition amounts were 0.1%, 0.5% and 1.0% (V / V). Take no Tween as a control.

[0033]The seeds were cultured for two generations, and each generation was carried out in a 50mL / 250mL Erlenmeyer flask with an inoculum volume of 8% (V / V), and cultured on a shaking table at 25°C and 200rpm for 48h. The second-gen...

Embodiment 2

[0039] Embodiment 2: the impact of Tween species on the fermentation of Schizochytrium

[0040] The composition and culture method of Schizochytrium culture medium are as described in Example 1.

[0041] Tween 20, Tween 40, Tween 60 or Tween 80 were respectively added to the fermentation medium of Schizochytrium, and the addition amount was 1.0% (V / V). Take no Tween as a control.

[0042] Experimental results such as figure 2 , Table 3 and Table 4 show:

[0043] Table 3 The effect of Tween species on the content and yield of total lipid and DHA

[0044]

[0045] Table 4 Effect of Tween species on fatty acid composition of Schizochytrium

[0046]

[0047] The results showed that Tween 20 and Tween 40 with the addition of 1.0% (V / V) significantly promoted the biomass of Schizochytrium, which were 3.26g / L and 36.34g / L respectively compared with the control group. 3.53g / L. The four kinds of Tweens all had a significant effect on improving total lipid accumulation, and...

Embodiment 3

[0049] Example 3: Effect of Mixed Components on Schizochytrium Fermentation

[0050] The composition and culture method of Schizochytrium culture medium are as described in Example 1.

[0051] Add Tween 20, Tween 40, Tween 60 and Tween 80 to the fermentation medium of Schizochytrium at the same time, and the addition amount is 0.25% (V / V). Take no Tween as a control.

[0052] The experimental results are shown in Table 5 and Table 6

[0053] Table 5 Effect of mixed components on fermentation parameters

[0054]

[0055] Table 6 Effect of mixed components on fatty acid composition of Schizochytrium

[0056]

[0057] The results showed that adding the four Tweens mixed into the culture medium could also increase the biomass, total lipid content and DHA production; the change of fatty acid distribution was smaller than that of single addition.

[0058] It can be seen that the present invention actually changes the physiological properties of microorganisms, improves the...

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Abstract

The invention discloses a culture medium added with a surfactant and application thereof. The culture medium added with the surfactant is composed of glycerinum or glucose which is taken as a major carbon source, a yeast extract and / or sodium glutamate which are / is taken as a major nitrogen source, inorganic salt components comprising Na2SO4, KH2PO4, K2HPO4, K2SO4 and MgSO4.7H2O, vitamins VB1, VB6 and VB12, and Tween 20, Tween 40, Tween 60 or Tween 60 added separately, wherein the concentration of the glycerinum or the glucose is 10-120g / L, and the concentration of the yeast extract and / or the sodium glutamate is 5-50g / L. The surfactant of an appropriate concentration is added to the culture medium so that the cell membrane permeability of microorganisms is changed, mass transfer of a substrate and oxygen is strengthened and growth is promoted; due to the adopted technical scheme, the conversion rate of the substrate can be increased without extra labor or equipment investment, and therefore, the fermentation of schizochytrium limacinum is effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to a culture medium added with a surfactant and its application in the fermentation of Schizochytrium fungus. Background technique [0002] Docosahexaenoic acid (22:6n-3) (DHA) is an important omega-3 series polyunsaturated fatty acids (omega-3 polyunsaturated fatty acids), which can promote infant intelligence and vision development, prevent cardiovascular Disease, anti-cancer, anti-inflammation, enhance immunity and other effects. Deep-sea fish oil is the traditional main source of omega-3 polyunsaturated fatty acids, but there are many problems, such as unstable quality, exogenous pollution, bad smell, high purification cost, etc. EPA and cholesterol contained in fish oil also make it unsuitable Added to maternity and infant food. On the contrary, microbial oils (single cell oils, SCO) are not only easy to achieve large-scale production, but also o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
Inventor 常明李婧刘睿杰王兴国金青哲黄健花邹孝强王小三刘元法
Owner JIANGNAN UNIV
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