Method for co-producing squalene and polyunsaturated fatty acid
A technology of unsaturated fatty acids and squalene, which is applied in the field of co-production of squalene and polyunsaturated fatty acids, can solve the problems of low EPA efficiency and achieve the effect of increasing the content
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Embodiment 1
[0030] In the present invention, the Schizochytrium sp. screened and isolated from seawater is used as the starting strain, and mutagenesis is performed to obtain a Schizochytrium sp. that can simultaneously produce squalene and polyunsaturated fatty acids. .) CABIO-A-2-IV, which has been deposited, and its deposit information is as follows:
[0031] The deposit number is CCTCC NO: M 2021565; the classification name is: Shchizochy trium sp.; the depository unit is: China Center for Type Culture Collection; deposit address: Wuhan University, Wuhan, China, postcode: 430072; the deposit date is May 19, 2021 .
Embodiment 2
[0033] The present embodiment adopts the Schizochytrium sp. (Schizochytrium sp.) CABIO-A-2-IV obtained in Example 1 to ferment, and the specific process is as follows:
[0034] (1) Seed Activation Cultivation: Get the Schizochytrium CABIO-A-2-IV obtained in Example 1 of the present invention and inoculate it into the activation medium for cultivation. The cultivation temperature is 28°C, and the shaking speed of the shaking table is 200r / min. Time 48h, the activation medium is: glucose 4%, sodium glutamate 3%, yeast extract 0.6%, KH 2 PO 4 0.6%, MgSO 4 0.8%, NaCl 2%, CaCl 2 0.03%, pH natural.
[0035] (2) Seed expansion cultivation: inoculate the activated seed culture solution of the shaking cultivation in the above step (1) into the expansion medium according to the inoculation amount of 5% (volume ratio) for cultivation, the cultivation temperature is 28°C, the cultivation time is 48h, and the culture medium is shaken. The shaking speed is 200 rpm, and the expansion m...
Embodiment 3
[0041] The present embodiment adopts the Schizochytrium sp. (Schizochytrium sp.) CABIO-A-2-IV obtained in Example 1 to ferment, and the specific process is as follows:
[0042](1) Seed Activation Cultivation: Get the Schizochytrium mutant strain of the present invention and inoculate it into the activation medium for cultivation, the cultivation temperature is 28°C, the shaking speed of the shaker is 200r / min, and the cultivation time is 48h. For: Dextrose 4%, Monosodium Glutamate 3%, Yeast Extract 0.6%, KH 2 PO 4 0.6%, MgSO 4 0.8%, NaCl 2%, CaCl 2 0.03%, pH natural.
[0043] (2) Seed expansion cultivation: inoculate the activated seed culture solution of the shaking cultivation in the above step (1) into the expansion medium according to the inoculation amount of 5% (volume ratio) for cultivation, the cultivation temperature is 28°C, the cultivation time is 48h, and the culture medium is shaken. The shaking speed is 200 rpm, and the expansion medium in the shake flask ...
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