Method for producing DHA through fermentation

A fermentation medium and by-product technology, applied in the field of fermentation and production of DHA, can solve problems such as poor oxidation stability, achieve the effects of improving production efficiency, facilitating growth and product accumulation, and reducing production costs

Active Publication Date: 2014-08-06
HUBEI XINHE BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the common DHA products currently on the market still have the disadvantage of poor oxidation stability. According to the

Method used

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  • Method for producing DHA through fermentation
  • Method for producing DHA through fermentation

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Experimental program
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Effect test

Embodiment 1

[0033] This embodiment relates to a method for producing DHA by fermentation, comprising the steps of:

[0034] Step 1, preparing seed culture medium;

[0035] The components of the seed medium are, in every 1L medium, glucose 50.0g, yeast extract 5.0g, peptone 3.0g, MgSO 4 2.0g, KH 2 PO 4 2.0g, the balance is water; pH is 6.0, sterilized at 121°C for 30min;

[0036] Step 2, preparing fermentation medium;

[0037] (a) Glucose 100.0g / L, yeast extract powder 15.0g / L, chlorophyll 1g / L, VB 1 0.1g / L, VB 12 0.1g / L, VB 6 0.1g / L, MSG 8g / L, glutamic acid 4g / L, biotin 0.1g / L, β-carotene 0.1g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, Na 2 SO 4 12.0g / L, sea crystal 15g / L, the balance is water, and the pH is controlled at 5.5-6.5 during the fermentation process.

[0038] (b) Corn processing by-products 120g / L, glucose 20g / L, yeast extract 5g / L, MgSO 4 1.0g / L, KH 2 PO 4 3.0g / L, Na 2 SO 4 12.0g / L, sea crystal 15g / L, the balance is water, and the pH is natural during the fermentation...

Embodiment 2

[0049] This embodiment relates to a method for producing DHA by fermentation, comprising the steps of:

[0050] Step 1, preparing seed culture medium;

[0051] The components of the seed medium are, in every 1L medium, glucose 50.0g, yeast extract 5.0g, peptone 3.0g, MgSO 4 2.0g, KH 2 PO 4 2.0g, the balance is water; pH is 6.0, sterilized at 121°C for 30min;

[0052] Step 2, preparing fermentation medium;

[0053] The components of the fermentation medium are as follows: every 1L medium contains 120g of corn processing by-products, 20.0g of glucose, 10.0g of yeast extract, MgSO 4 1.0g, KH 2 PO 4 3.0g, Na 2 SO 4 12.0g, sea crystal 15g, the balance is water; sterilize at 121°C for 30min, cool down for later use;

[0054] Step 3, shake flask seed cultivation and seed expansion cultivation;

[0055] Take a glycerol tube of 1 mL of Schizochytrium sp. frozen in a refrigerator at -80 °C, inoculate it into a 500 mL Erlenmeyer flask containing 200 mL of the above seed medium,...

Embodiment 3

[0061] This embodiment relates to a method for producing DHA by fermentation, comprising the steps of:

[0062] Step 1, preparing seed culture medium;

[0063] The components of the seed medium are, in every 1L medium, glucose 40.0g, yeast extract 4.0g, peptone 2.0g, MgSO 4 1.0g, KH 2 PO 4 1.0g, the balance is water; pH is 6.0, sterilized at 121°C for 30min;

[0064] Step 2, preparing fermentation medium;

[0065] The components of the fermentation medium are as follows: every 1L medium contains 80g of corn processing by-products, 10.0g of glucose, 5.0g of yeast extract, MgSO 4 0.5g, KH 2 PO 4 2.0g, Na 2 SO 4 5.0g, sea crystal 5g, the balance is water; sterilize at 121°C for 30min, cool for later use;

[0066] Step 3, shake flask seed cultivation and seed expansion cultivation;

[0067] Take a glycerol tube of 1 mL of Schizochytrium sp. frozen in a refrigerator at -80 °C, inoculate it into a 500 mL Erlenmeyer flask containing 200 mL of the above seed medium, and cult...

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Abstract

The invention relates to a method for producing DHA through fermentation and belongs to the field of microbial fermentation. The method comprises the following steps: using by-products obtained from maize processing as raw material, and adopting schizochytrium sp. for fermentation, so that the product DHA is obtained. In the whole fermentation process of the method, the adjustment and control of pH value are not required, the production cost is reduced, and the production efficiency is improved; by adopting the method provided by the invention, all components in the by-products obtained from maize processing can be used to the maximum extent, and the growth of microorganisms and product accumulation are facilitated. By adopting the method, the content of DHA in products prepared finally is increased obviously, the fatty acid in the DHA is changed obviously, the content of hexadecanoic acid is increased obviously and can reach 45.8%, an unexpected technical effect is achieved, meanwhile, the content of DHA in the products is increased obviously and can reach 47%, the oxidation stability of DHA grease is improved obviously, and the oxidation induction time is prolonged to 21.2 h from 1.1 h, and can reach 22.6 h to the maximum, so that the unexpected technical effect is produced.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for producing DHA by fermentation. Background technique [0002] Schizochytrium limacimum is mainly obtained from the marine environment. In order to meet its suitable growth conditions and the demand for a large amount of DHA oil synthesis, and to realize the large-scale fermentation production of Schizochytrium, it is generally selected to use a medium with extremely complex components for cultivation. , the patent of authorized notification number CN101519676A discloses the components of the fermentation medium, its composition is complex, using glucose, yeast extract, sodium sulfate, sodium phosphate, dipotassium hydrogen phosphate, corn steep liquor, chlorophyll, VB1, VB12, monosodium glutamate, sea salt, Composite medium composed of magnesium sulfate heptahydrate, glutamic acid, beta carotene, VB6, etc., the composition of the medium is complex, th...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/645
Inventor 李二虎钱凯王鲁峰杨启伟梅天勇
Owner HUBEI XINHE BIOTECH
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