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Application of catechinic acid in improving DHA grease yield of schizochytrium limacinum

A technology of Schizochytrium and catechin is applied in the field of microorganisms to achieve the effects of reducing production costs and increasing oil content

Active Publication Date: 2021-03-12
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the control of Schizochytrium DHA oil content by adding catechins

Method used

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  • Application of catechinic acid in improving DHA grease yield of schizochytrium limacinum
  • Application of catechinic acid in improving DHA grease yield of schizochytrium limacinum

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Experimental program
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Effect test

Embodiment 1

[0041] The strain used in the present invention is Schizochytrium ATCC-20888 (purchased from the American Type Culture Collection (American Type Culture Collection, ATCC) as wild-type Schizochytrium, preserved in a glycerol tube. The present invention can also use other Any wild-type Schizochytrium was equally effective.

[0042] Seed medium: D-glucose 40g / L, yeast extract 2g / L, sodium glutamate 10g / L, MgCl 2 3g / L, CaCl 2 2H 2 O 1g / L, KH 2 PO 4 4g / L, KCl 2g / L, NaCl 15g / L, MgSO 4 ·7H 2 O 5g / L, FeCl 3 0.1g / L.

[0043] Fermentation medium: D-glucose 40g / L, yeast extract 2g / L, sodium glutamate 10g / L, MgCl 2 3g / L, (NH 4 ) 2 SO 4 6g / L, KH 2 PO 4 4g / L, KCl 2g / L, NaCl 15g / L, MgSO 4 ·7H 2 O 5g / L, FeCl 3 0.1g / L, catechin 1.5mmol / L.

[0044] The method for cultivating Schizochytrium schizochytrium with catechins to improve oil production comprises the following steps:

[0045] 1. Inoculate the preserved Schizochytrium into 30mL seed culture medium, the volume rati...

Embodiment 2

[0061] Using the method of Example 1, the catechins added in the fermentation medium follow a certain concentration gradient (0.3mmol / L, 1mmol / L, 1.2mol / L, 1.5mol / L, 1.8mol / L, 2mol / L, 3mol / L) was added, the fermentation was stopped at 24 hours, and various indicators were tested. The results are shown in Table 1.

[0062] Table 1

[0063]

[0064] As shown in Table 1, after adding different concentrations of exogenous catechins, the biomass of the cells increased, and the addition of 1.5mM catechins had the highest oil content in Schizochytrium, which could effectively increase the The content of DHA oil produced by fermentation, wherein the DHA output is increased by more than 70%, the ratio of DHA to biomass (mg / g) is increased by more than 40%, and the total oil is increased by more than 70%. In addition, the production of β-carotene therein is also significantly increased accordingly. In the present invention, the amount of catechin added is very important, and the e...

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Abstract

The invention discloses application of catechin in improvement of DHA (docosahexaenoic acid) grease yield of schizochytrium limacinum, the grease yield is improved by culturing the schizochytrium limacinum with the catechin, and a small amount of catechin in a specific proportion is added to a schizochytrium limacinum fermentation system to inhibit generation of PEPC (polyepichlorohydrin) enzyme.Therefore, a metabolic network is disturbed to reduce biosynthesis of carbon flowing to lipid, the content of grease produced by schizochytrium limacinum fermentation can be effectively increased, theDHA yield is increased by 70% or above, the ratio of DHA to biomass is increased by 40% or above, the total grease is increased by 70% or above, and the beta carotene yield is also remarkably increased. Through simple fermentation regulation and control, the contents of DHA grease and beta carotene in the microbial schizochytrium limacinum can be remarkably increased, so that the production costis reduced, and the method has economic benefits.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a method for increasing the oil fermentation yield of Schizochytrium fungi, in particular to the application of catechins in increasing the yields of Schizochytrium fungus DHA oil and beta carotene. Background technique [0002] Docosahexaenoic acid (DHA) is an important long-chain polyunsaturated fatty acid (PUPA), belonging to Omega-3 unsaturated fatty acids, which can be used as an essential component of cell membrane synthesis, not only for the growth and functional development of the baby's brain Vital, and more easily absorbed by the brain than other fatty acids, DHA can significantly improve learning ability. In addition, DHA can lower blood lipids, prevent and treat diseases such as atherosclerosis, phenylketonuria, and occasional Alzheimer's disease. Therefore, DHA has attracted widespread attention. But at present the DHA production in the world is in short supply and e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12P23/00C12N1/14C12N1/38C12R1/645
CPCC12P7/6427C12P23/00C12N1/14C12N1/38Y02E50/10
Inventor 孙小曼许莹霜杜菲马旺贾雨雷
Owner NANJING NORMAL UNIVERSITY