Immobilized microorganism preparation method

A technology for immobilizing microorganisms and microorganisms, which is applied in the preparation field of biochemical technology, can solve the problems of short life of embedding carriers, low microbial activity, and difficulty in showing superiority, and achieves small activity damage, simple preparation process, and long-term use. The effect of longevity

Active Publication Date: 2010-01-20
山东青素环保材料有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problem is that the microbial activity after embedding is low, and it is difficult to show superiority in wastewater treatment effect; the life of the embedding carrier is short, and the operating time generally does not exceed 6 months

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Step 1, prepare solution;

[0020] 1. 12g of polyethylene glycol prepolymer was used to obtain solution A;

[0021] 2. Add 4g of activated sludge to solution A, then add 1g of N,N-dimethylbisacrylamide and 0.05g of silicon dioxide (N,N-dimethylbisacrylamide and silicon dioxide are added in no order sequence), then add 0.2g diethoxyacetophenone to obtain solution B;

[0022] 3. Add water to solution B until the volume of the solution is 100ml, and mix well to obtain the desired solution;

[0023] In step 2, the solution prepared in step 1 is placed under an ultraviolet lamp to cure for 4 minutes, cut and washed to obtain immobilized microorganisms.

Embodiment 2

[0025] Step 1, prepare solution;

[0026] 1. Take 15g of polyethylene glycol prepolymer to obtain solution A;

[0027] 2. Add 4.5g of activated sludge to solution A, then add 0.837g of N,N-dimethylbisacrylamide and 0.08g of silicon dioxide, and then add 0.121g of diethoxyacetophenone to obtain solution B;

[0028] 3. Add water to solution B until the volume of the solution is 100ml, and mix well to obtain the desired solution;

[0029] In step 2, the solution prepared in step 1 is placed under an ultraviolet lamp to cure for 5 minutes, cut and washed to obtain immobilized microorganisms.

Embodiment 3

[0031] Step 1, prepare solution;

[0032] 1. 18g polyethylene glycol prepolymer was used to obtain solution A;

[0033] 2. Add 5g of activated sludge to solution A, then add 0.5g of N,N-dimethylbisacrylamide and 0.1g of silicon dioxide, and then add 0.05g of diethoxyacetophenone to obtain solution B;

[0034] 3. Add water to solution B until the volume of the solution is 100ml, and mix well to obtain the desired solution;

[0035] In step 2, the solution prepared in step 1 is placed under an ultraviolet lamp to cure for 6 minutes, cut and washed to obtain immobilized microorganisms.

[0036] Implementation effect: the embedded bacteria prepared in the above examples can be used continuously for more than 8 months in the aerated bioreactor.

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Abstract

The invention relates to an immobilized microorganism preparation method in biochemical technical field, comprising the following steps: step 1, preparing a solution of which preparation method per 100ml comprises the following steps: (1) dissolving 12-18g of polyethylene glycol prepolymer in water to obtain a solution A; (2) adding microorganism in the solution A, then adding 0.5-1g of N,N-dimethylbisacrylamide and 0.05-0.1g of silicon dioxide, then adding 0.05-0.2g of diethoxyacetophenone and obtaining a solution B; (3) adding water in the solution B to ensure the volume of the obtained solution is 100ml, mixing evenly and obtaining the desired solution; step 2, placing the solution obtained in the step 1 under a ultraviolet lamp for curing, cutting, and washing to obtain the immobilized microorganism. The method of the invention can be performed at room temperature, the reaction process is easy to control and the prepared embedded bacteria particles have longer service life.

Description

technical field [0001] The invention relates to a preparation method in the field of biochemical technology, in particular to a preparation method of immobilized microorganisms. Background technique [0002] So far, the immobilization and embedding methods of microorganisms and bacteria reported in the literature generally adopt physical embedding or traditional chemical reaction methods. Physically embedded active bacteria are easily disturbed by external conditions and fall off or are impacted by external toxic substances, etc., thereby affecting the treatment effect. However, the traditional chemical embedding method generally uses a large amount of initiators and cross-linking agents. The curing process is more severe, which has a greater impact on the activity of bacteria, and the inactivation of bacteria is more serious. According to statistics, the activity of bacteria after embedding is lower than 20% of the activity before embedding, which greatly weakens the advan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08C12N11/04
Inventor 乔向利刘哲刘志伟曾颖林
Owner 山东青素环保材料有限责任公司
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