43results about How to "Accurate introduction" patented technology

The invention discloses a method for site-specific mutagenesis of medicago sativa genes by employing a CRISPR/Cas9 system. First of all, a binary expression vector MsCRISPR/Cas9 is constructed; the vector is designed and constructed for a target gene; then, agrobacterium tumefaciens-mediated transformation is used to achieve site-specific mutagenesis of a specific gene, and a mutant transformed plant with the target gene knocked out is obtained by screening; the method uses the CRISPR/Cas9 technology for cross-pollination autotetraploid plant medicago sativa for the first time, and obtains a mutant plant; a MtU6 promoter is used for the first time to initiate sgRNA transcription in the medicago sativa, thereby improving the transcription efficiency; the mutation rate can reach 52%; the method realizes direct mutation on the coding sequence of the gene, and opens up a new field for gene editing in the medicago sativa, laying a technical foundation for simultaneous silencing of a plurality of genes, deletion of large chromosome fragments, precise insertion of foreign genes into targets, gene regulation and the like.

The invention relates to methods of producing eukaryotic cell libraries encoding a repertoire of binding molecules (“binders”), wherein the methods use a site-specific nuclease for targeted cleavage of cellular DNA to enhance site-specific integration of binder genes through endogenous cellular repair mechanisms. Populations of eukaryotic cells are produced in which a repertoire of genes encoding binders are integrated into a desired locus in cellular DNA (e.g., a genomic locus) allowing expression of the encoded binding molecule, thereby creating a population of cells expressing different binders.

The invention discloses a magnetic suspension rotor harmonic current inhibition method based on parallel FORC and phase lag-lead compensation, and the method comprises the steps: firstly building a magnetic suspension rotor dynamical model comprising quality imbalance and sensor harmonic; secondly employing a magnetic suspension rotor harmonic current inhibition method based on a parallel FORC structure. The structure enables internal models of a harmonic at all frequencies to be converted into a plurality of parallel units with the similar structures, and quickly improves the dynamic performances of a system. The method also employs the phase lag-lead compensation to improve the stability and dynamic performances of the system. The invention proposes a method for quickly and adaptively adjusting the controller gain for a specific harmonic signal. The method achieves the dynamic optimization of the dynamic performances and stability. The method achieves the inhibition of a harmonic component of a current of a magnetic bearing coil in a magnetic suspension rotor, and is suitable for the harmonic current inhibition of the magnetic suspension rotor system with the quality imbalance and sensor harmonic.

The invention provides improved systems, devices, and methods for analyzing a large number of sample compounds contained in standard multi-well microtiter plates or other array structures. The multi-well plates travel along a conveyor system to a test station having a microfluidic device. At the test station, each plate is removed from the conveyor and the wells of the multi-well plate are sequentially aligned with an input port of the microfluidic device. After at least a portion of each sample has been input into the microfluidic channel system, the plate is returned to the conveyor system. Pre and/or post testing stations may be disposed along the conveyor system, and the use of an X-Y-Z robotic arm and novel plate support bracket allows each of the samples in the wells to be input into the microfluidic network through a probe affixed to a microfluidic chip. A clamshell structure having a hinged lid can releasably support the chip while providing and/or accommodating the electrical, optical, structural, and other interface connections between the microfluidic device and the surrounding system.

The invention relates to a multifunctional tourist attraction service system with a team organization function and a realization method. Based on a communication function, a GPS (Global Position System) positioning function, a network connection function, a voice input and playing function, an information display and operation function and the like on an intelligent mobile terminal, the intelligent mobile terminal is connected with a network server and a processor to form the multifunctional tourist attraction service system with the team organization function; the service system is used for carrying out real-time dynamic monitoring on users and providing a series of service supports including supports on positioning and navigation of the users, tourist attraction information display and broadcasting and salvation services, so that a leader or a tour guide can conveniently manage and lead, and furthermore, the management efficiency and the communication efficiency are improved; and the service system has abundant tourism functions, can provide functions and services of accurate navigation, introduction, route recommending, reminding of lagging behind, warning of dangerous zones, tourist attraction congestion reminding and the like, and can bring relatively great convenience for the users in tourist attractions.

The invention discloses a magnetic suspension rotor odd harmonic vibration inhibition method based on a hybrid odd repetition controller. The magnetic suspension rotor odd harmonic vibration inhibition method comprises the following steps: firstly, establishing a magnetic suspension rotor kinetic model containing rotor mass unbalance and sensor harmonic; secondly, adopting the magnetic suspensionrotor odd harmonic vibration inhibition method based on the hybrid odd repetition controller (hereinafter referred to as HORC). The HORC is composed of an odd harmonic frequency inhibited second-orderinner film structure and a modified resonant controller (hereinafter referred to as MRSC), which are connected in parallel. The HORC can be used for improving a harmonic inhibition capability of a magnetic suspension rotor system when the harmonic frequency is perturbed, and the control robustness of a system under the harmonic frequency perturbation of the system is improved. According to the magnetic suspension rotor odd harmonic vibration inhibition method based on the hybrid odd repetition controller, an odd harmonic current component generated by a magnetic bearing coil in a magnetic suspension rotor is inhibited, so that odd harmonic vibration of the magnetic suspension rotor system with the rotor mass unbalance and the sensor harmonic is inhibited.

A conventional large-scale parallel pyrosequencing apparatus has the disadvantage that throughput decreases because much time and a large number of procedures are required for introduction of measurement beads, and analysis accuracy is deteriorated due to a reduction in accuracy of reagent replacement. There is provided an apparatus, wherein the apparatus includes a flow cell having a plurality of microfabricated reactors, and a camera opposed thereto, and DNA to be measured is fixed on the surfaces of beads having a specific gravity of 4 or greater, preferably zirconia beads. The flow cell is made horizontal when introducing the beads into the flow cell, and opposed to an optical axis of the camera when measuring an elongation reaction, the optical axis of the camera having a gradient with respect to the horizontal direction.

The invention discloses a sagger automatic continuous charging device for an electronic kiln, and belongs to the technical field of electronic kiln auxiliary facilities. The sagger automatic continuous charging device comprises a rack, a spiral material conveying tube, a material storage box and a power transmission mechanism. The rack is supported on a terrace. The spiral material conveying tube is fixed on the top of the rack, a screw rod shaft is matched in a tube cavity of the spiral material conveying tube, and a spiral sheet is formed on the screw rod shaft. A closed end is formed at the left end of the spiral material conveying tube, and an opened end is formed at the right end of the spiral material conveying tube. The material storage box is fixedly connected with the top of the rack, and the bottom of a material storage box cavity is communicated with the tube cavity of the spiral material conveying tube. The power transmission mechanism is connected with the screw rod shaft. The sagger automatic continuous charging device for the electronic kiln is characterized in that the opened end of the spiral material conveying tube is connected with a bend connector in a matched mode, the bend connector is communicated with the tube cavity, a bend connector discharging opening of the bend connector faces downwards, the left end of the screw rod shaft stretches out of the tube cavity to form a screw rod shaft extension shaft section, the screw rod shaft extension shaft section is supported on the top of the rack, and the power transmission mechanism is connected with the screw rod shaft extension shaft section. Discharging smoothness is guaranteed, the spiral sheet can be prevented from scraping the inner wall of the tube cavity, and accordingly materials can be accurately led into a sagger.

The invention relates to the field of liquid crystal display technologies, in particular to a liquid crystal display panel which comprises a driving switch array substrate, a color optical filter substrate, a liquid crystal layer and frame adhesive. The driving switch array substrate comprises a display area and a non-display area, and the non-display area is arranged on the periphery of the display area and is further provided with a first spacing portion for spacing the frame adhesive; the color optical filter substrate is opposite to the driving switch array substrate; the liquid crystal layer is arranged between the driving switch array substrate and the color optical filter substrate and is positioned in the display area; the frame adhesive encircles the liquid crystal layer and is arranged between the driving switch array substrate and the color optical filter substrate; the frame adhesive is positioned in the non-display area and is arranged on the first spacing portion. The liquid crystal display panel has the advantages that frame adhesive spacing components are arranged on the first substrate, and accordingly the frame adhesive coating accuracy can be improved.

The invention is suitable for the field of computers, and provides a commodity information management method. The method comprises the steps that position information of commodities on a shelf and parameter information of the commodities are input into a system in advance, when a consumer requires to display some commodities in a live broadcast process, the system actively or an anchor selects and calls the position information and the parameter information of the commodities and sends the information to a prompt end, and the anchor can see the parameter information of the commodities from the prompt end, so that the anchor can introduce the commodities more accurately; the anchor can accurately position the desired commodities on the shelf according to the position information, so that the time for searching a target commodity from a plurality of commodities is shortened, the anchor has more time for effective sales output in one live broadcast, the live broadcast effect is better, and the satisfaction degree of a commodity supplier or a consumer is improved.