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High-performance liquid phase detection method for heart-calming granules

A detection method, high-efficiency liquid phase technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of simple operation, good stability, and accurate measurement

Active Publication Date: 2016-05-04
SHANDONG BUCHANG PHARMA +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the 2015 edition of the Chinese Pharmacopoeia is only for notoginseng saponin R in Wenxin Granules. 1 , Ginsenoside Rg 1 , Ginsenoside Rb 1 Quantitative detection was carried out on the content of Codonopsis pilosula in the literature granule, but no content detection was done on the monarch drug Codonopsis pilosula

Method used

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  • High-performance liquid phase detection method for heart-calming granules
  • High-performance liquid phase detection method for heart-calming granules
  • High-performance liquid phase detection method for heart-calming granules

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Embodiment 1

[0029] Determination of the contents of notoginsenoside R1, ginsenoside Rg1, tangshenoside, ginsenoside Rb1 and ginsenoside Rd in Wenxin Granules.

[0030] 1. Instruments and reagents

[0031] Instrument: Angilent1260 high performance liquid chromatography, DAD detector.

[0032] Reagents: Acetonitrile is chromatographically pure, water is ultrapure water, and other reagents are analytically pure. Reference substances: Panax notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Rd were purchased from the Central Inspection Institute, with a purity of 95%; Codonopsis was purchased from Yifang Science and Technology, with a purity of 98%.

[0033] 2. Selection of chromatographic conditions:

[0034] Column: AmethystC 18 -H (4.6×250mm, 4μm); mobile phase: Acetonitrile is mobile phase A, water is mobile phase B;

[0035] The present invention adopts the gradient elution method, and the elution sequence is: 0-14min, 22%-30%A; 14-35min, 30%-38%A; 35-45min, 38%-38%...

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Abstract

The invention provides a high-performance liquid phase detection method for heart-calming granules. The method comprises the step of adopting a high-performance liquid phase method for measuring the content of notoginsenoside R1, the content of ginsenoside Rg1 and the content of ginsenoside Rb1, and the step of adopting the high-performance liquid phase method for measuring the content of ginsenoside Rd and the content of lobetyolin. On the basis of utilizing the high-performance liquid phase method for measuring the content of notoginsenoside R1, the content of ginsenoside Rg1 and the content of ginsenoside Rb1, the high-performance liquid phase method is additionally adopted for measuring the content of ginsenoside Rd and the content of lobetyolin, and therefore the quality control index for the heart-calming granules is more comprehensive, and the detection method is good in precision, stability and repeatability.

Description

technical field [0001] The invention relates to a high-efficiency liquid phase detection method for Wenxin Granules, which belongs to the field of detection and analysis of Chinese patent medicines. Background technique [0002] Wenxin Granule is a traditional Chinese medicine preparation made by extracting and processing five traditional Chinese medicines such as Codonopsis Radix, Rhizoma Polygonatum, Radix Notoginseng, Amber and Gansong. Wait. Functions and indications are: nourishing qi and nourishing yin, promoting blood circulation and removing blood stasis. It is used for palpitations, restlessness, shortness of breath, fatigue, chest tightness and pain, ventricular premature beats, and atrial premature beats caused by Qi and Yin deficiency and blood stasis. [0003] Because Wenxin Granule has a good therapeutic effect in the treatment of arrhythmia diseases. However, the 2015 edition of the Chinese Pharmacopoeia is only for notoginseng saponin R in Wenxin Granules....

Claims

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Application Information

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IPC IPC(8): G01N30/86G01N30/74G01N30/06
CPCG01N30/06G01N30/74G01N30/8634
Inventor 赵步长朱彦吴红华王益民南国南景一王勇吴建涛
Owner SHANDONG BUCHANG PHARMA
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