Applications of isoflavone compound
A technology of isoflavones and compounds is applied to the application field of isoflavones in the preparation of antiviral drugs, which can solve the problem that the active ingredients are not completely clear, and achieve significant anti-HIV-1 virus activity, significant anti-HIV virus activity, Significant effect of anti-CVB3 virus activity
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Embodiment 1
[0041] The preparation of embodiment 1 calycosin
[0042] Collect 500 g of feces from experimental rats administered with acteocoside by gavage, extract 3 times with 5 times the amount of methanol, combine the obtained methanol extracts, evaporate the methanol to dryness, and then adsorb through 101 macroporous resin. Then, 101 macroporous resins were first eluted with 30% ethanol, then 101 macroporous resins were eluted with 50% ethanol and fractions were collected, and finally 101 macroporous resins were regenerated with 90% ethanol. Afterwards, the obtained fractions were evaporated to dryness, adsorbed on Sephadex LH-20 dextran gel, and eluted with ethanol. After thin-layer chromatography, the eluents containing the same components were combined to obtain three parts of A, B and C. Fraction, the obtained fraction B is adsorbed on silica gel H60, after dichloromethane-methanol (100:1-5:1, volume ratio) eluent elution, and after enrichment by semi-preparative liquid chromato...
Embodiment 2
[0043] The preparation of embodiment 2 calycosin
[0044] Actascoisoflavone was treated with 1M H under acidic conditions 2 SO 4 After hydrolysis, the anion exchange resin Amberlite (OH - ) separation to obtain calycosin (m / z: 285 [M+H] + ; 13C-NMR (DMSO-d6, 100MHz) δ (ppm): 174.53 (C-4), 162.48 (C-7), 157.33 (C-9), 152.98 (C-2), 147.47 (C-4') , 146.00(C-3'), 127.24(C-5), 124.68(C-1'), 123.33(C-3), 119.65(C-6'), 116.62(C-10), 116.42(C- 2'), 111.95(C-5'), 102.05(C-8), 55.65(OMe))
Embodiment 3
[0045] Embodiment 3 antiviral activity test
[0046] 1. Culture medium and culture medium
[0047] Cell culture medium: containing 10% fetal bovine serum, product of Tianjin Haoyang Biological Products Technology Co., Ltd.; DMEM (high glucose) medium, RPMI-1640 and fetal bovine serum were purchased from Gibco, USA.
[0048] RPMI-1640 complete medium: containing 10% fetal bovine serum, 2 mM L-glutamine, 10 mM HEPES, 50 μM 2-mercaptoethanol (purchased from Bio-Rad), 100,000 IU penicillin and 100 μg / ml streptomycin.
[0049] 2. Cells and viruses
[0050] Human laryngeal carcinoma epithelial cells (Hep-2) were purchased from the Cell Center of the Institute of Basic Medicine, Peking Union Medical College, China. Coxsackie virus B type 3 (CoxB3) Nancy strain was routinely passaged by Cui Xiaolan's laboratory of the Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, half of the tissue culture infectious dose (50% Tissue culture infectious dose, TCID 5...
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