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Building method for fingerprint of radix astragali seu hedysari pill with effect of reinforcing middle warmer

A technology of fingerprint spectrum and construction method, applied in the field of traditional Chinese medicine fingerprint spectrum, can solve problems such as not individual chemical components, and achieve the effects of quality and curative effect assurance, scientific evaluation of quality, and short time-consuming.

Active Publication Date: 2017-07-14
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, most of the raw material components such as astragaloside IV and paeoniflorin are determined for quality control by liquid chromatography, but this method only detects the corresponding raw material composition, and it is not the real drug effect. Individual chemical components are the sum of the components in the overall compound, so it is necessary to construct the fingerprint of Huangqi Jianzhong Pills and control its quality from the perspective of the whole

Method used

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  • Building method for fingerprint of radix astragali seu hedysari pill with effect of reinforcing middle warmer
  • Building method for fingerprint of radix astragali seu hedysari pill with effect of reinforcing middle warmer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of embodiment 1 need testing solution

[0028] 1. Sampling volume of the test product

[0029] Weigh 0.1g, 0.15g and 0.2g of the ground powder of Huangqi Jianzhong Pills, add 1ml of methanol solution, weigh, ultrasonically treat (power 250W, frequency 25kHz) for 20min, centrifuge at 13000rpm for 15min, and take the supernatant through a 0.22μm micropore The test sample was prepared by membrane filtration.

[0030] The results show that when the sampling amount is 0.1g, 0.15g and 0.2g, there is little difference in the collection of chromatograms (not shown), and considering the protection of the chromatographic column, 0.1g is selected as the sampling amount of the test product.

[0031] 2. Investigation of extraction solvent

[0032] Water and methanol were used as extraction solvents respectively, and the same batch of test samples were investigated by ultrasonic extraction.

[0033] The results show that the chromatographic peak with too high respo...

Embodiment 2

[0034] Embodiment 2 Chromatographic conditions and system adaptability test investigation

[0035] 1. Selection of fingerprint detection method

[0036] Literature research shows that the main components contained in Huangqi Jianzhong Pills are flavonoids and triterpenoid saponins, all of which have good ultraviolet absorption. Therefore, UPLC-UV detection method is selected for fingerprint research.

[0037] 2. Selection of wavelength for fingerprint detection

[0038] Literature research shows that the active ingredients contained in Huangqi Jianzhong Pill Qiwei medicine have different ultraviolet absorption wavelength characteristics, so a diode array detector is used to scan the samples at full wavelength.

[0039] The results showed that at 254nm wavelength, the amount of information was large and the chromatographic peaks were easy to observe. Therefore, 254nm was finally determined as the detection wavelength of the fingerprint of Huangqi Jianzhong Pills.

[0040] 3. ...

Embodiment 3

[0058] Example 3 A method for constructing the chemical composition fingerprint of Huangqi Jianzhong Pills, comprising the following steps:

[0059] (1) Preparation of reference substance solution: take appropriate amount of calycocetin, formononetin and paeoniflorin reference substance, accurately weighed, add appropriate amount of methanol to make each 1ml contains 40.8 μg calycocetin, 34.8 μg formononetin and 32.7 μg Mixed reference solution of paeoniflorin.

[0060] (2) Preparation of the test solution: Take a Huangqi Jianzhong Pill, add liquid nitrogen and quickly grind to powder, accurately weigh 0.1g, add 1ml methanol, weigh, ultrasonically extract (power 250W, frequency 25kHz) for 20min, make up Volumetric weight, 13000rpm, centrifuge at 4°C for 15min, filter the supernatant with a 0.22μm microporous membrane, and use it as the test solution.

[0061] (3) Determination: Precisely draw 10 μl of each of the reference substance and the test solution, inject it into an ultr...

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Abstract

The invention provides a building method for a fingerprint of a radix astragali seu hedysari pill with the effect of reinforcing middle warmer. The building method comprises the following steps: 1) preparing a reference methanol solution from a proper amount of calycosin, fermlononetin and paeoniflorin; 2) preparing a test article methanol solution from the radix astragali seu hedysari pill with the effect of reinforcing middle warmer; 3) injecting 10muml of the reference methanol solution and the test article methanol solution into an ultra performance liquid chromatograph, and performing gradient elution under the condition of ultra performance liquid chromatography (UPLC) to obtain a fingerprint, wherein the operating conditions of the liquid chromatograph are as follows: a moving phase is prepared from acetonitrile and a 0.1-percent methanoic acid aqueous solution, the flow speed of the moving phase is 0.3ml / min, the column temperature is 30 DEG C, and the detection wavelength is 254nm. The built fingerprint can clearly reflect the peak outlet positions of feature peaks of effective components in the radix astragali seu hedysari pill with the effect of reinforcing middle warmer, and can be used for controlling the quality of the radix astragali seu hedysari pill with the effect of reinforcing middle warmer.

Description

technical field [0001] The invention relates to traditional Chinese medicine fingerprints, in particular to a method for constructing the fingerprints of Huangqi Jianzhong Pills. Background technique [0002] With the development of modern drug analysis technology, fingerprint technology is an internationally recognized quality control mode for controlling the quality of traditional Chinese medicine. Chinese medicine fingerprint is based on the systematic research of chemical components of traditional Chinese medicine, and is mainly used to evaluate Chinese medicinal materials and traditional Chinese medicine compound preparations Excellent quality, it provides a full range of quality assurance in the cultivation and introduction of raw medicinal materials, the standardization and optimization of the production process of Chinese patent medicines, and the formulation of product quality standards. The establishment of TCM fingerprints can more comprehensively reflect the type...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 刘月涛胡英还秦雪梅张丽增
Owner SHANXI UNIV
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