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Characteristic chromatogram of Dingkundan as well as construction method and application of characteristic chromatogram

A feature map and construction method technology, applied in the field of construction of traditional Chinese medicine feature map, can solve the problems of inaccurate quality monitoring, lack of inclusion, etc., achieve the effect of convenient operation, increase the consistency rate, and reduce the possibility of manual processing

Active Publication Date: 2022-01-11
INNER MONGOLIA JINGXIN PHARMACEUTICAL CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to overcome the defect that the characteristic peaks in the Dingkundan fingerprint spectrum in the prior art do not contain the characteristic components in precious medicinal materials such as saffron, and there may be a risk of inaccurate quality monitoring, and provide a The characteristic map of Zhongdingkundan and its construction method and application

Method used

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  • Characteristic chromatogram of Dingkundan as well as construction method and application of characteristic chromatogram
  • Characteristic chromatogram of Dingkundan as well as construction method and application of characteristic chromatogram
  • Characteristic chromatogram of Dingkundan as well as construction method and application of characteristic chromatogram

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] The construction of embodiment 1 Dingkun Dan (big honey pill) HPLC characteristic collection of illustrative plates

[0085] 1.1 Raw material

[0086] Dingkun Dan (Big Honey Pill): batch numbers are 190101, 190132, 190133, 190341, 190165, 190412, 190725, 190828, 190829, 191013, 191038, 191039, 191222; each pill weighs 10.8g, Inner Mongolia Jingxin Pharmaceutical Co., Ltd. company.

[0087] 1.2 Equipment and model

[0088] High performance liquid chromatography: Agilent1260Infinity, DAD detector, Agilent Technologies Co., Ltd.;

[0089] Chromatographic column: Inertsil ODS-3C18 (4.6×250mm, 5μm), Japan GLSciences Company;

[0090] Analytical balance: METTLERTOLEDOAL204 (1 / 10,000), Mettler-Toledo Instruments (Shanghai) Co., Ltd.;

[0091] Analytical balance: METTLERTOLEDOXS105 (1 / 100,000), Mettler Toledo Instruments (Shanghai) Co., Ltd.;

[0092] Electronic balance: UTP-313, d=0.01g, Shanghai Huachao Electric Co., Ltd.;

[0093] Pharmacopoeia sieve: No. 3 sieve, Zhej...

Embodiment 2

[0121] Example 2 Dingkundan Control Characteristic Spectrum and Similarity Evaluation

[0122] Get Dingkundan (big honey pill), operate by the preparation and assay method of need testing solution in embodiment 1, continuous sample injection 6 times, based on the chromatogram of above-mentioned 13 batches of samples, use the pharmacopoeia committee to recommend "Chinese medicine chromatographic feature map similarity evaluation system" (2008 edition) carries out data processing and obtains common pattern feature map, is Dingkundan contrast feature map R (such as figure 2 As shown), the retention time of each main chromatographic peak of the 6 injection samples is basically the same, and it is determined that the 9 peaks separated by this detection condition are their common chromatographic peaks.

[0123] The analysis was carried out according to the established Dingkundan characteristic map analysis method, and the similarity was calculated according to the similarity evalua...

Embodiment 3

[0126] Embodiment 3 methodological verification

[0127] The HPLC chromatographic conditions determined in Example 1 were investigated for repeatability and stability.

[0128] (1) Repeatability

[0129] Get Dingkun Dan (big honey pill), operate under the preparation and assay method of need testing solution according to embodiment 1, calculate its similarity by Chinese medicine chromatographic fingerprint spectrum similarity evaluation system, the results are shown in Figure 5 , Image 6 .

[0130] Figure 5-6 Middle: S1 is repeatability 1, S2 is repeatability 2, S3 is repeatability 3, S4 is repeatability 4, S5 is repeatability 5, S6 is repeatability 6, and R is the control fingerprint.

[0131] The results showed that the similarity was greater than 0.95, indicating that the method had good repeatability.

[0132] (2) Stability

[0133] Get Dingkun Dan (big honey pill), operate under the preparation and assay method of need testing solution according to embodiment 1, ...

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Abstract

The invention discloses a characteristic spectrum of Dingkundan as well as a construction method and application of the characteristic spectrum. The characteristic chromatogram of the Dingkundan comprises nine common peaks, the fifth peak is ferulic acid, the seventh peak is crocin-I, and the eighth peak is ligustilide. The construction method of the characteristic spectrum of Dingkundan comprises the following steps: carrying out gradient elution separation on a to-be-detected solution of Dingkundan by virtue of a high performance liquid chromatography, wherein the filler of the chromatographic column is octadecylsilane chemically bonded silica, the detection wavelength is 260 to 280 nm, methanol is used as a mobile phase A, and 0.05-0.5% formic acid is used as a mobile phase B; according to the present invention, the characteristic peak of the Dingkundan characteristic spectrum contains crocin-I, such that the characteristic components in the precious medicinal materials such as saffron in the Dingkundan can be effectively monitored, and the quality of the Dingkundan can be accurately monitored and reflected. In addition, the Dingkundan component characteristic spectrum construction method provided by the invention is convenient to operate, simple, convenient and rapid.

Description

technical field [0001] The invention relates to a construction method of a characteristic map of traditional Chinese medicine, in particular to a characteristic map of Dingkun Dan, its construction method and application. Background technique [0002] Dingkundan is composed of red ginseng, antler, saffron, Panax notoginseng, white peony root, Rehmannia glutinosa, angelica, Atractylodes macrocephala, medlar, scutellaria baicalensis, Cyperus cyperi, coriander, Chuanxiong, antler cream, donkey-hide gelatin, and Corydalis etc. The medicinal taste is processed, which can nourish qi and blood, regulate menstruation and relieve depression. The formula is complex and has many medicinal flavors. In order to maintain the stable efficacy of different batches of this large compound Chinese medicine, strict internal control quality standards must be formulated. The 2015 edition of "Chinese Pharmacopoeia" stipulates that the quality control standards of Dingkundan include: Microscopic id...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/74G01N30/8686Y02A50/30
Inventor 刘保国胡万徐建军李艳艳
Owner INNER MONGOLIA JINGXIN PHARMACEUTICAL CO LTD
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