Method for simultaneously detecting multiple index components in vigor-preserving decoction preparation

A detection method, the technology of Baoyuantang, which is applied in the directions of measuring devices, material separation, and analysis of materials, etc., can solve the problems of simultaneous detection of multiple index components in Baoyuantang preparations, achieve comprehensive and effective control, and improve detection efficiency , good reproducibility

Active Publication Date: 2020-07-24
HEBEI SHINEWAY PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem that the existing detection method cannot realize the simultaneous detection of multiple index components in the Baoyuan Decoction preparation, the present invention provides a simultaneous detection method for multiple index components in the Baoyuan Decoction preparation

Method used

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  • Method for simultaneously detecting multiple index components in vigor-preserving decoction preparation
  • Method for simultaneously detecting multiple index components in vigor-preserving decoction preparation
  • Method for simultaneously detecting multiple index components in vigor-preserving decoction preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] 1. Solution preparation

[0063] 1.1 Preparation of the test solution

[0064] S1, mix 3.75g ginseng, 7.5g astragalus, 1.88g licorice, and 0.75g cinnamon, add 10 times the amount of water, soak in a ceramic pot for 30min, bring to a boil over high heat, keep boiling on low heat, continue to decoct for 40min, and filter while hot , concentrate under reduced pressure at 60°C, and dilute to 25mL with water to obtain Baoyuan decoction;

[0065] S2. Accurately measure 1 mL of Baoyuan Tang water decoction and add it to a measuring bottle, add 6 times the volume of absolute ethanol, vortex for 3 minutes, centrifuge at 12000 rpm for 10 minutes, take the supernatant and evaporate to dryness, and dilute to a 2 mL measuring bottle with methanol , take the continued filtrate, get the detection of ginsenoside Rg 1 , Ginsenoside Re, Ginsenoside Rb 1 The test solution of other index components;

[0066] S3, accurately measure 5 mL of the decoction of Baoyuan Tang and add it to an ...

Embodiment 2

[0084] Methodology Validation:

[0085] 2.1 Specificity

[0086] Mixed reference substance solution: take an appropriate amount of reference substance stock solution of each index component, mix, dilute with methanol, and constant volume to make ginsenoside Re and ginsenoside Rg 1 , Ginsenoside Rb 1 The concentrations were 200 μg·mL -1 ; The concentrations of acteosin glucoside, acteosin, liquiritin, apiloisoliquiritin, monoammonium glycyrrhizinate, and cinnamic acid were 250 μg·mL -1 ; The concentration of isoliquiritin is 100 μg·mL -1 mixed reference solution.

[0087] Inject and analyze the test solution prepared in 1.1, the mixed reference solution prepared above, and the negative control solution prepared under 1.3 according to the above ultra-high performance liquid chromatography conditions.

[0088] in,figure 1 For mixing reference substance solution (a), lacking ginseng negative need testing solution (k), full prescription need testing solution (c); the chromatog...

Embodiment 3

[0116] Get 16 batches of Baoyuan Decoction samples, prepare the test solution according to the preparation operation of the test solution under 1.1 in Example 1, and measure by ultra-high performance liquid chromatography conditions, record the peak area of ​​each component, and adopt the external standard According to the peak area of ​​each component, the active ingredient content in each batch of samples was calculated. The results of content determination are shown in Table 5.

[0117] table 5

[0118]

[0119]

[0120] As can be seen from the above table, the ginsenoside Rg extracted from different batches of raw material components 1 / Re, Ginsenoside Rb 1 , acteosin glucoside, acteosin, liquiritin, glycyrrhizinic acid, apiloisoliquiritin, isoliquiritin and cinnamic acid are quite different, therefore, it is necessary to quantify these components in Baoyuan Decoction This is of great significance to the quality control of Baoyuan Decoction.

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Abstract

The invention provides a method for simultaneously detecting multiple index components in a vigor-preserving decoction preparation. The vigor-preserving decoction preparation comprises the following index components: ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, calycosin-7-glucoside, calycosin, liquiritin, glycyrrhizic acid, isoliquiritin apioside, isoliquiritin and cinnamic acid. Detection is conducted according to the following high performance liquid chromatography conditions: a chromatographic column is an octadecyl bonded silica gel chromatographic column; the detection wavelength ofa DAD detector is 201-205 nm, 258-262 nm, 288-292 nm and 358-362 nm; and the mobile phase A is acetonitrile, the mobile phase B is an ammonium acetate buffer solution, and gradient elution is conducted. According to the provided detection method, effective separation of the multiple index components can be realized, and the provided method is found to be sensitive, accurate and relatively good inreproducibility through methodology research and verification of specificity, sensitivity and the like.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a simultaneous detection method for multiple index components in Baoyuan decoction preparations. Background technique [0002] Baoyuan Decoction comes from "Concise Medicine" (1629) compiled by Ming Sun Zhihong. It is an ancient classic prescription. White, restful sleep and miscellaneous syndromes, all belong to weakness and should be taken.” Its prescription is composed of ginseng, astragalus, licorice, and cinnamon, and a piece of ginger is added when decocting. As a traditional Chinese medicine preserved in the process of inheritance and development of traditional Chinese medicine One of the outstanding representatives of prescriptions, it has been widely used in modern clinics, mainly for the treatment of coronary heart disease, chronic renal failure, aplastic anemia, hepatitis B, leukopenia and other diseases. [0003] However, at present, the quality control of Bao...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8679G01N2030/062
Inventor 李晓波张敏王梦月李寅庆侯金才秦玉霞
Owner HEBEI SHINEWAY PHARMA
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