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Fingerprint construction method and quality detection method for Radix Astragali and Chinese date oral liquid

The technology of Qizao oral liquid and fingerprint spectrum is applied in the field of fingerprint spectrum construction and quality detection of Qizao oral liquid, which can solve the problems of complex components, difficult separation, qualitative and quantitative components, etc., and achieves strong stability, comprehensive accuracy, etc. The effect of quick detection

Active Publication Date: 2017-11-21
中山市恒生药业有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

Qizao Oral Liquid is a compound traditional Chinese medicine preparation with complex components. It is obviously not enough to quantify a single component as the quality control index of Qizao Oral Liquid according to the routine, and it is difficult to separate qualitatively and quantitatively multiple types of components at the same time under conventional chromatographic conditions.

Method used

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  • Fingerprint construction method and quality detection method for Radix Astragali and Chinese date oral liquid
  • Fingerprint construction method and quality detection method for Radix Astragali and Chinese date oral liquid
  • Fingerprint construction method and quality detection method for Radix Astragali and Chinese date oral liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening of the Fingerprint Construction Method of Qizao Oral Liquid

[0026] 1. Experimental materials

[0027] The 10 batches of Qizao oral liquid used in the experiment were provided by Zhongshan Hengsheng Pharmaceutical (batch numbers: 20130901, 20130902, 20130903, 20131001, 20131002, 20131003, 20131101, 20131102, 20131103, 20131104).

[0028] 2. Instrument

[0029] High-performance liquid chromatograph: Waters 2695 high-performance liquid chromatograph (American waters technology company); Quaternary pump, diode array detector (PAD2998), automatic sampler, Empower chromatographic workstation. Column: SHISEIDO-Capcell PAK-C 18 Chromatographic column (4.6mm×250mm, 5μm); balance: one-thousandth balance (Swiss Mettler ME203E), one-thousandth balance (Swiss Mettler ME204), one-hundred-thousandth balance (Swiss Mettler MS105); Ultrasonic generator (Kunshan Ultrasonic KQ-700DE40KHz280W); Ultrapure water system: Millipore, USA. Methanol, n-butanol, ethyl ace...

Embodiment 2

[0085] 7. Establishment and Analysis of Standard Fingerprints

[0086] (1) Collecting fingerprints

[0087] Take 10 batches of Qizao oral liquid and prepare it into a test solution respectively, and analyze it according to the chromatographic conditions determined in Example 1. Take formononetin as a reference peak, and record the fingerprints, see image 3 .

[0088] (2) Establishment of common patterns and determination of similarity

[0089] The fingerprints of 10 batches of Qizao oral liquid samples were imported into "Zhongzhi Pharmaceutical Group Traditional Chinese Medicine Fingerprint Database Software" to generate the common pattern of Qizao oral liquid fingerprints by the median method. A total of 12 common peaks were calibrated, and the common pattern was calculated. Compared with the reference substance, peak No. 2 was identified as protocatechuic acid; Peak No. 4 was Verbasidone glucoside; Peak No. 6 was Formononetin; Peak No. 9 was Verbasidone; Peak No. 10 For...

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Abstract

The invention relates to a fingerprint construction method and a quality detection method for a Radix Astragali and Chinese date oral liquid. The fingerprint construction method comprises the following steps: taking protocatechuic acid, calycosin-7-glucoside, ononin, calycosin, genistein and formononetin as reference substances, and carrying out gradient elution with 50% aqueous acetonitrile solution-0.2% aqueous phosphoric acid solution as a mobile phase; and carrying out segmented wavelength detection, and recording and obtaining the fingerprint. The quality detection method comprises the following steps: constructing standard fingerprints of 10 or more batches of the Radix Astragali and Chinese date oral liquid through the fingerprint construction method, and carrying out consistence comparison on the standard fingerprints and the fingerprint of a sample to be detected in order to detect the quality of the sample to be detected. Compared with the prior art, the methods have the advantages of high specificity and stability, realization of full, accurate and quick detection of the quality of the finished or semi-finished product of the Radix Astragali and Chinese date oral liquid, and suitableness for large-scale production and application.

Description

technical field [0001] The invention relates to a method for constructing fingerprints of Qizao oral liquid and its quality detection method. Background technique [0002] The prescription of Qizao Oral Liquid contains four traditional Chinese medicines: Astragalus, Jujube, Poria, and Periwinkle Radix. components such as flavonoids and flavonoids. It has the effects of replenishing qi and blood, strengthening the spleen and stomach, and is mainly used for leukopenia, physical weakness after illness, and decreased immunity caused by liver loss. Qizao Oral Liquid is a compound traditional Chinese medicine preparation with complex components. It is obviously not enough to quantify a single component as the quality control index of Qizao Oral Liquid by conventional methods, and it is also difficult to separate qualitatively and quantitatively multiple types of components at the same time under conventional chromatographic conditions. Therefore, it is very necessary to study a ...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 刘星云彭丽华成金乐徐吉银唐琳劳悦富陈炜璇陈金梅
Owner 中山市恒生药业有限公司
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