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Application of calycosin in radix astragali in preparing medicament for vascular protection and angiogenesis promotion

A technology of isoflavone and angiogenesis, applied in the field of medicine

Inactive Publication Date: 2010-02-24
UNIVERSITY OF MACAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Calycosin is an important isoflavone active compound isolated from capsular Astragalus, which has anti-ischemic damage, blocks calcium ion channels, relaxes blood vessels, and relieves endothelial cell damage caused by angiotensin II (Ang II). Injury and other vascular protective effects, there is no report on the relationship between calycosin and vascular endothelial cell activity

Method used

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  • Application of calycosin in radix astragali in preparing medicament for vascular protection and angiogenesis promotion
  • Application of calycosin in radix astragali in preparing medicament for vascular protection and angiogenesis promotion
  • Application of calycosin in radix astragali in preparing medicament for vascular protection and angiogenesis promotion

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preparation example Construction

[0020] The preparation of the calycosin medicament of the present invention can refer to the conventional drug preparation method, and the preparation of the drug is well known to those skilled in the art after the required dosage of the drug is known. The pharmaceutical dosage form is any dosage form approved by medicine, such as powder, injection, capsule, tablet or oral liquid.

[0021] The new application of calycosin in Radix Astragali of the present invention is further described below through experiments and accompanying drawings. All data are expressed as mean ± SEM, and statistical comparisons are performed using one-way analysis of variance and Tukey's test for comparison between groups.

[0022] Experimental drug: Calycocosin was purchased from Shanghai Traditional Chinese Medicine Standardization Research Center, dissolved in DMSO to make a stock solution with a concentration of 100mM, and stored at 4°C for later use. VEGF was purchased from R&D system (Minneapoli...

Embodiment 1

[0025] Example 1 GeneBLAzer β-lactam reporter gene model detects transcriptional activity of estrogen receptor α and β

[0026] The Effect of Calycosin on the Transcriptional Activity of Estrogen Receptor (ER) The SelectScreen provided by Invitrogen TM Cell-Based Nuclear Receptor Profiling Service is completed using GeneBLAzer β-lactamase reporter gene technology, which can measure the agonistic or antagonistic effect of the drug to be tested on nuclear receptors, and can sensitively detect the potency and selectivity of the drug to be tested . In this reporter gene system, the β-lactamase cDNA is linked to an upstream activator sequence (UAS), which is fused with the GAL4 transcription of the estrogen receptor ligand binding domain (ER (LBD)) The factor DNA-binding domain (GAL4(DBD)-ER(LBD)) binds to UAS when a ligand binds to ER(LBD), thereby controlling the transcription of β-lactamase. GeneBLAzer technology uses an optimized mammalian β-lactamase reporter gene (bla) and...

Embodiment 2X

[0035] Embodiment 2XTT method detects endothelial cell proliferation

[0036] Take HUVECs that grow to 90% confluence, wash and digest, and make cell suspension. After counting, press 1x10 4 per well in a 96-well plate. After culturing for 24 hours until the cells were completely adhered to the wall, remove the supernatant medium, add 100 μl of low-serum hormone-free medium (0.5% CS-FBS) to each well and incubate for 24 hours to synchronize the state of the cells. In the experimental group, different concentrations of calycosin (100 μM ~ 1 μM) were added to the groups, and each group had 8 replicate wells, and 200 μl / well of low serum culture medium containing the drug was added. At the same time, two groups of controls were set up, the blank control group was 0.1% DMSO culture solution, and the positive control was 20ng / ml VEGF culture solution.

[0037] XTT results showed that calycosin can promote the proliferation of HUVEC in a dose-dependent manner. Among them, when t...

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Abstract

The invention discloses an application of calycosin in radix astragali in preparing a medicament for vascular protection. Angiogenesis is promoted by vascular endothelial cell proliferation, , and further a good vascular protection effect is generated; the prepared medicament used as a selective estrogen modulator can replace estrogen, promote the angiogenesis, and protect the vascular endothelialcells, thereby treating cardiovascular diseases (such as atherogenesis and the like).

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of calycosin in Astragalus membranaceus in the preparation of blood vessel protection medicine. Background technique [0002] Angiogenesis refers to the process by which adults form new blood vessels by sprouting on the basis of the original vascular plexus or existing blood vessels. It has the function of maintaining the normal growth and balance of the human body. play an important role in prognosis. In recent years, the research on regulating angiogenesis has become a hot spot in clinical treatment. Angiogenesis mainly involves the degradation of basement membrane, migration, proliferation and tube formation of endothelial cells, among which the proliferation of endothelial cells is one of the most important indicators of angiogenesis. At the same time, endothelial cell dysfunction and death are important pathological processes of various cardiovascular dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P9/00A61P5/30A61P9/10
Inventor 李铭源唐菁燕黎晌胡光李振华张在军
Owner UNIVERSITY OF MACAU
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