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Determination method of content of calycosin glucoside in stable pulvis for strengthening body resistance and detoxifcation

A technique for the isoflavone and Fuzheng Jiedu powder, which is applied in the field of high performance liquid chromatography and can solve the problem of not specifying the method for determining the content of the isoflavone glucoside and the like

Inactive Publication Date: 2013-03-27
HENAN KANGXING PHARMA
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Problems solved by technology

[0005] The present invention aims to solve the problem of determining the content of acteosin glucoside in Fuzheng Jiedu powder, and solve the problem of determining the content of acteocoside glucoside which is not specified in the Veterinary Pharmacopoeia

Method used

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  • Determination method of content of calycosin glucoside in stable pulvis for strengthening body resistance and detoxifcation
  • Determination method of content of calycosin glucoside in stable pulvis for strengthening body resistance and detoxifcation
  • Determination method of content of calycosin glucoside in stable pulvis for strengthening body resistance and detoxifcation

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Experimental program
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Embodiment 1

[0039] Embodiment one Batch No. 20120417 Fuzheng Jiedu Powder Determination of Calycosin Glucoside Content

[0040] Determination according to high performance liquid chromatography (Chinese Veterinary Pharmacopoeia).

[0041] Detection instruments Elite high performance liquid chromatography, EC2000 ultraviolet detector.

[0042] Chromatographic column General C18 (200mm×4.6mm, 5μm) liquid chromatography column. The filler is SinoChrom ODS-BP.

[0043] Chromatographic conditions and system suitability test Acetonitrile-2% formic acid solution (20:80) was used as the mobile phase; the detection wavelength was 260nm. The number of theoretical plates should not be less than 3000 based on the peak of calycosin glucoside.

[0044] Preparation of reference substance solution Accurately weigh 0.1042g of acteocoside glucoside reference substance, add 200ml of methanol to make a solution containing 51.01μg of acteocoside glucoside per 1ml, and obtain it.

[0045] Preparation of ...

Embodiment 2

[0049] Embodiment two Determination of the content of calycosin glucoside in Fuzheng Jiedu powder with batch number 20120605

[0050] Determination according to high performance liquid chromatography (Chinese Veterinary Pharmacopoeia).

[0051] Detection instruments Elite high performance liquid chromatography, EC2000 ultraviolet detector.

[0052] Chromatographic column General C18 (200mm×4.6mm, 5μm) liquid chromatography column. The filler is SinoChrom ODS-BP.

[0053] Chromatographic conditions and system suitability test Acetonitrile-2% formic acid solution (20:80) was used as the mobile phase; the detection wavelength was 260nm. The number of theoretical plates should not be less than 3000 based on the peak of calycosin glucoside.

[0054] Preparation of reference substance solution Accurately weigh 0.1042g of acteocoside glucoside reference substance, add 200ml of methanol to make a solution containing 51.01μg of acteocoside glucoside per 1ml, and obtain it.

[005...

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Abstract

The invention discloses a determination method of the content of calycosin glucoside in stable pulvis for strengthening body resistance and detoxification; an acetonitrile-0.2% formic acid solution (20:80) is used as a mobile phase; a detection wavelength is 260 nm; calycosin glucoside is used as a control; a sample is treated by methanol reflux, filtration, drying by distillation, and dissolution; and the content of calycosin glucoside in the pulvis for strengthening body resistance and detoxification is detected by a high performance liquid chromatography-ultraviolet detector. The method has good linear relation, good precision and reproducibility, can be used as a determination method of the content of calycosin glucoside in pulvis for strengthening body resistance and detoxification, and is simple, rapid, and easy to operate.

Description

technical field [0001] The invention relates to a high-performance liquid chromatography method, in particular to a content determination technology of traditional Chinese medicine components. Background technique [0002] Fuzheng Jiedu Powder is a prescription preparation composed of astragalus, Radix Radix and Epimedium three traditional Chinese medicines in a certain proportion according to the provisions of Fuzheng Jiedu Powder in Part II of the Veterinary Drug Code of the People's Republic of China. Evil, heat-clearing and detoxifying effects. Indications chicken bursal disease. However, the quality standard under it does not specify the identification method and content determination method of each active ingredient. [0003] In order to improve the quality standard of Fuzheng Jiedu Powder and provide a basis for the formulation of its quality standard, the present invention explores the content determination method of the component calycocoside glucoside of Astragal...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 于瑞刘瑞丽刘海新刘琴王林
Owner HENAN KANGXING PHARMA
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