Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Determination method of content of calycosin glucoside in stable pulvis for strengthening body resistance and detoxifcation

A technique for the isoflavone and Fuzheng Jiedu powder, which is applied in the field of high performance liquid chromatography and can solve the problem of not specifying the method for determining the content of the isoflavone glucoside and the like

Inactive Publication Date: 2013-03-27
HENAN KANGXING PHARMA
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problem of determining the content of acteosin glucoside in Fuzheng Jiedu powder, and solve the problem of determining the content of acteocoside glucoside which is not specified in the Veterinary Pharmacopoeia

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Determination method of content of calycosin glucoside in stable pulvis for strengthening body resistance and detoxifcation
  • Determination method of content of calycosin glucoside in stable pulvis for strengthening body resistance and detoxifcation
  • Determination method of content of calycosin glucoside in stable pulvis for strengthening body resistance and detoxifcation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment one Batch No. 20120417 Fuzheng Jiedu Powder Determination of Calycosin Glucoside Content

[0040] Determination according to high performance liquid chromatography (Chinese Veterinary Pharmacopoeia).

[0041] Detection instruments Elite high performance liquid chromatography, EC2000 ultraviolet detector.

[0042] Chromatographic column General C18 (200mm×4.6mm, 5μm) liquid chromatography column. The filler is SinoChrom ODS-BP.

[0043] Chromatographic conditions and system suitability test Acetonitrile-2% formic acid solution (20:80) was used as the mobile phase; the detection wavelength was 260nm. The number of theoretical plates should not be less than 3000 based on the peak of calycosin glucoside.

[0044] Preparation of reference substance solution Accurately weigh 0.1042g of acteocoside glucoside reference substance, add 200ml of methanol to make a solution containing 51.01μg of acteocoside glucoside per 1ml, and obtain it.

[0045] Preparation of ...

Embodiment 2

[0049] Embodiment two Determination of the content of calycosin glucoside in Fuzheng Jiedu powder with batch number 20120605

[0050] Determination according to high performance liquid chromatography (Chinese Veterinary Pharmacopoeia).

[0051] Detection instruments Elite high performance liquid chromatography, EC2000 ultraviolet detector.

[0052] Chromatographic column General C18 (200mm×4.6mm, 5μm) liquid chromatography column. The filler is SinoChrom ODS-BP.

[0053] Chromatographic conditions and system suitability test Acetonitrile-2% formic acid solution (20:80) was used as the mobile phase; the detection wavelength was 260nm. The number of theoretical plates should not be less than 3000 based on the peak of calycosin glucoside.

[0054] Preparation of reference substance solution Accurately weigh 0.1042g of acteocoside glucoside reference substance, add 200ml of methanol to make a solution containing 51.01μg of acteocoside glucoside per 1ml, and obtain it.

[005...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a determination method of the content of calycosin glucoside in stable pulvis for strengthening body resistance and detoxification; an acetonitrile-0.2% formic acid solution (20:80) is used as a mobile phase; a detection wavelength is 260 nm; calycosin glucoside is used as a control; a sample is treated by methanol reflux, filtration, drying by distillation, and dissolution; and the content of calycosin glucoside in the pulvis for strengthening body resistance and detoxification is detected by a high performance liquid chromatography-ultraviolet detector. The method has good linear relation, good precision and reproducibility, can be used as a determination method of the content of calycosin glucoside in pulvis for strengthening body resistance and detoxification, and is simple, rapid, and easy to operate.

Description

technical field [0001] The invention relates to a high-performance liquid chromatography method, in particular to a content determination technology of traditional Chinese medicine components. Background technique [0002] Fuzheng Jiedu Powder is a prescription preparation composed of astragalus, Radix Radix and Epimedium three traditional Chinese medicines in a certain proportion according to the provisions of Fuzheng Jiedu Powder in Part II of the Veterinary Drug Code of the People's Republic of China. Evil, heat-clearing and detoxifying effects. Indications chicken bursal disease. However, the quality standard under it does not specify the identification method and content determination method of each active ingredient. [0003] In order to improve the quality standard of Fuzheng Jiedu Powder and provide a basis for the formulation of its quality standard, the present invention explores the content determination method of the component calycocoside glucoside of Astragal...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02
Inventor 于瑞刘瑞丽刘海新刘琴王林
Owner HENAN KANGXING PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com