Content detection method for determining effective components in longshengzhi capsule by HPLC-QQQ/MS method

A detection method and technology for active ingredients, applied in the field of chemical analysis and detection of Chinese patent medicines, can solve problems such as unclear material basis and inability to comprehensively reflect the quality of traditional Chinese medicines, and achieve the effect of rapid detection

Active Publication Date: 2019-02-05
SHAANXI BUCHANG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there are relatively many clinical studies on Longshengzhi Capsules, and the clinical effect is remarkable. However, the research on its chemical composition has not been reported, and its material basis is still unclear. Therefore, the determination of the chemical composition of Longshengzhi Capsules can effectively evaluate the quality of compound preparations. Whether it is to ensure the safety and effectiveness of medication, or to accurately and comprehensively evaluate the quality of medicinal materials, it

Method used

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  • Content detection method for determining effective components in longshengzhi capsule by HPLC-QQQ/MS method
  • Content detection method for determining effective components in longshengzhi capsule by HPLC-QQQ/MS method
  • Content detection method for determining effective components in longshengzhi capsule by HPLC-QQQ/MS method

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Embodiment 1

[0071] Example 1: HPLC-QQQ / MS method established by the present invention to determine the content of active ingredients in Longshengzhi Capsules. 1 Instruments and reagents

[0072] 1.1 Instruments

[0073] Agilent1260 high performance liquid chromatograph, Agilent6470 triple quadrupole tandem mass spectrometer (Agilent, USA); analytical balance (Mettler, Switzerland); 3-18KS centrifuge (Sigma, Germany); KQ-500E ultrasonic cleaner (Kunshan City) Ultrasound Instrument Co., Ltd.).

[0074] 1.2 Reagents

[0075] Reference substance Astragaloside IV (H-013-170614), Hydroxysafflor yellow A (Q-008-170303), Verbasin glucoside (M-020-170929), Verbasin (M-021-170517), bitter Amygdalin (K-001-161216), Ferulic acid (A-002-161216), Dehydroxylactone (Q-016-170816), Syringin (C-009-161217), Acanthopanax senticosin E(C-008-171229), Amygdalin (K-001-161216), Paeoniflorin (S-010-170214), Oxypaeoniflorin (Q-019-180315), Benzoyl Paeoniflorin (B-024) -171216), ligustrazine (C-045-171216), ligu...

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Abstract

The invention provides a content detection method for determining effective components in a longshengzhi capsule by an HPLC-QQQ/MS method. The chromatograph condition is that the chromatographic column takes a octadecyl-bonded silica gel column as a filling agent, 0.1% formic acid-water as a mobile phase A, 0.1% formic acid-acetonitrile as a mobile phase B, and the volume ratio of the mobile phaseA and the mobile phase B is 80% to 0%:20 to 100%, so that gradient elution is performed. According to the content detection method for determining the effective components in the longshengzhi capsuleby the HPLC-QQQ/MS method, the effective substance components of traditional Chinese medicines such as astragaloside A, hydroxysafflor yellow A, calycosin 7-o-glucoside, calycosin, ferulic Acid, syringin, n-butylphthalide, ligustilide, senkyunolide A, senkyunolide I, senkyunolide H, ligustrazine, isofraxidin, dehydrocostus lactone, amygdalin, syringin E, paeoniflorin, oxypaeoniflora, benzoylpaeoniflorin, etc., in the longshengzhi capsule can be determined. The method has the advantages of being simple in operation method, high in sensitivity and accurate in content.

Description

technical field [0001] The invention relates to the field of chemical analysis and detection of Chinese patent medicines, in particular to a method for detecting the content of effective components in Longshengzhi capsules by HPLC-QQQ / MS method. Background technique [0002] Longsheng Zhi Capsule is exclusively produced by Shaanxi Buchang Pharmaceutical Co., Ltd. The drug is composed of astragalus, leech, chuanxiong, angelica, peach kernel, safflower, red peony, muxiang, calamus, dilong, mulberry and acanthopanax The compound preparation composed of 12 traditional Chinese medicines has the effects of invigorating qi and promoting blood circulation, removing blood stasis and dredging collaterals. Pre-market clinical studies have shown that the total effective rate of Longshengzhi Capsules for cerebral infarction can reach 91.7%, and the improvement rate of common TCM syndromes of stroke sequelae is above 80%. After listing, it has been widely used in the treatment of cerebra...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 王伟肖红斌赵步长魏紫奕赵涛徐文娟王益民
Owner SHAANXI BUCHANG PHARMA
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