Capturing sequences adjacent to type-IIS restriction sites for genomic library mapping
Patent Information
- Authority / Receiving Office
- US · United States
- Current Assignee / Owner
- AFFYMETRIX INC
- Publication Date
- 2006-10-05
- Estimated Expiration
- Not applicable · inactive patent
Smart Images
Figure 1 
Figure 2 
Figure 3
Abstract
Description
[0001] This application is a continuation-in-part of U.S. application Ser. No. 08 / 307,881, filed Sep. 16, 1994, which is hereby incorporated by reference in its entirety for all purposes.
[0002] Research leading to the present invention was funded in part by NIH grant Nos. 5-F32-HG00105 and RO1 HG00813-02, and the government may have certain rights to the invention.
[0003] The present invention generally relates to novel methods for isolating, characterizing and mapping genetic markers in polynucleotide sequences. More particularly, the present invention provides methods for mapping genetic material using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation. BACKGROUND OF THE INVENTION
[0004] The relationship between structure and funct...