DNA-based Information Storage Method

Abstract

The invention relates to the technical field of information storage, in particular to a DNA-based information storage method. A long sequence in vivo DNA information storage technique is provide. Themain goal is to construct a coding system with strong error correction mechanism based on LDPC and BCH codes, which can reduce the redundancy of primers and indexes by long sequence coding, and achieve a high actual carrying capacity (above 97%). The main goal is to construct a medium-length DNA sequence (above 1Kbp). Saccharomyces cerevisiae in vivo assembly system is used to assemble and store long sequences and preserve information, and the information can be replicated at low cost, high fidelity and high speed by model organisms such as Saccharomyces cerevisiae, Escherichia coli or Bacillus. At the same time, because of the existence of strong error correction system, the data in the bacterial cell can be reduced perfectly with low coverage (1-5 X) in the second generation and third generation sequencing.

Description

technical field [0001] The invention relates to the technical field of information storage, in particular to a DNA-based information storage method. Background technique [0002] Human society has entered the era of information explosion, and has higher requirements for storage media and storage systems. According to the survey jointly conducted by International Data Corporation and EMC, the total amount of global data is growing rapidly at an annual growth rate of 50%. In 2017, the total amount of global data has reached 16ZB, which is equivalent to the total amount of global data ten years ago. 100 times. According to the survey estimates, by 2020, the total amount of global data will reach 44ZB, which is equivalent to the combined storage capacity of 45,000 existing Amazon data storage centers. [0003] Modern digital computers store, communicate, and manipulate binary data, often represented as 0s and 1s. The storage and transmission of these bits of information are l...

AI Technical Summary

Problems solved by technology

[0006] The existing DNA information storage technology is limited by DNA synthesis technology, mainly focusing on short sequences (below 200bp), and its storage technology has the following disadvantages: simple encoding (error-correcting code) (mainly Huffman+RS error-correcting code), DNA sequence Low actual loading rate (caused by other invalid sequences such as primers, addresses, and redundancy, the actual loading rate is usually below 60%), high replication cost (PCR amplification requires a large amount of DNA polymerase when a large amount of replication is required, and the cost is high) and poor replication fidelity (high base mutation rate and possible uneven amplification during PCR)

Images