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A method for screening toxicity of traditional Chinese medicine by model organism zebra fish

A technology of zebrafish and traditional Chinese medicine, applied in the field of medicine, can solve the problems of few research reports on toxicity screening, lack of research data suitability and reliability, etc., and achieve the effect of strong practicability, fast speed and high accuracy

Inactive Publication Date: 2012-10-10
SHANGHAI BAINIAN SHIDANDE INSPECTION TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are some reports on using zebrafish to screen the efficacy of traditional Chinese medicine, but there are few research reports on toxicity screening. Even if there are some reports and related research models, there is a lack of relevant research data to evaluate the suitability and reliability of the model. and has become a well-established and accepted toxicity screening model

Method used

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  • A method for screening toxicity of traditional Chinese medicine by model organism zebra fish
  • A method for screening toxicity of traditional Chinese medicine by model organism zebra fish
  • A method for screening toxicity of traditional Chinese medicine by model organism zebra fish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take the ephedra medicinal material, grind it into powder after treatment, pass through a 60-120 mesh sieve, dissolve the clean powder in distilled water at a rate of 10g / L, soak at room temperature for 24 hours, filter it with gauze, and take dechlorinated tap water to prepare a test laboratory. The desired concentration group is set aside.

[0019] Zebrafish born in the same year were taken, with a body weight of 0.28-0.35 g / fish and a body length of 2.75-3.25 cm. Put the zebrafish in a glass tank filled with dechlorinated water, feed the special fish feed once in the morning and evening, and stop feeding 24 hours before the test. Put the ephedra solutions with different concentrations to be tested in each group in the glass jars, and then put the zebrafish into the glass jars containing the medicine, 10 in each jar, without feeding during the whole test period, the test temperature is 22 degrees, after the test Start to observe, observe this every 6 hours, observe f...

Embodiment 2

[0025] Take the extract of aconitum, grind it into powder after treatment, pass through a 60-120 mesh sieve, dissolve the clean powder in distilled water at 10g / L, soak at room temperature for 24 hours, filter with gauze, and take dechlorinated tap water to prepare The concentration group required for the test is reserved.

[0026] Zebrafish born in the same year were taken, with a body weight of 0.28-0.35 g / fish and a body length of 2.75-3.25 cm. Put the zebrafish in a glass tank filled with dechlorinated water, feed the special fish feed once in the morning and evening, and stop feeding 24 hours before the test. Put the ephedra solutions with different concentrations to be tested in each group in the glass jars, and then put the zebrafish into the glass jars containing the medicine, 20 in each jar, no food during the whole test period, the test temperature is 25 degrees, after the test Start to observe, observe this every 4 hours, observe for 120 hours, find that the dead a...

Embodiment 3

[0032] Take Shoutai pills, grind them into powder after treatment, pass through a 60-120 mesh sieve, dissolve the clean powder in distilled water at a rate of 10g / L, soak at room temperature for 24 hours, filter with gauze, and take dechlorinated tap water to prepare the test The desired concentration set is set aside.

[0033] Zebrafish born in the same year were taken, with a body weight of 0.28-0.35 g / fish and a body length of 2.75-3.25 cm. Put the zebrafish in a glass tank filled with dechlorinated water, feed the special fish feed once in the morning and evening, and stop feeding 24 hours before the test. Put the ephedra solutions with different concentrations to be tested in each group in the glass jars, and then put the zebrafish into the glass jars containing the medicine, 20 in each jar, no food during the whole test period, the test temperature is 25 degrees, after the test Start to observe, observe this every 4 hours, observe for 120 hours, find that the dead are f...

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Abstract

The purpose of the invention is to provide a method with easy operation and high accuracy for screening toxicity of traditional Chinese medicine by model organism zebra fish. The method comprises the following steps: dissolving a crushed or extracted traditional Chinese medicine material or a patented Chinese herbal prescription into water, preparing test solutions with different concentrations, selecting healthy and active zebra fish with similar sizes, and putting the zebra fish randomly into clean glass jars with an experimental temperature of (20+ / -2) DEG C, continuously observing for 4 hours after the experiment begins, simultaneously carrying out untime observation within 120 hours at a frequency of at least one time observation in every 6 hours, picking out dead fish timely, when the fish fries are poisoned to stop breathing, clamping the fishtail petioles with a pincette, determining that the fish fry is dead when no response is observed in 10 min], calculating and recording accumulated mortality of the zebra fish of each group, and calculating LC 50 and 95% confidence interval by utilizing a karbers method. The method is characterized by a simple operation method and a strong practicality; the method can preliminarily determine the toxicity of the traditional Chinese medicine with a fast speed and a high accuracy.

Description

technical field [0001] The invention relates to a method for screening the toxicity of traditional Chinese medicines by using the model organism zebrafish, which belongs to the technical field of medicine. Background technique [0002] The zebrafish is an important model organism for growth, development and genetic studies, and has become an important model organism for studying embryonic development in the past 20 years. At present, zebrafish has obtained relatively complete genomics, molecular genetics and embryology data, and has been widely used in the monitoring of environmental toxicants. It has been used in many chemical substances in the environment, such as harmful heavy metals, pesticides and halogenated aromatic compounds. Cumulative effects and toxicity studies of carcinogens. Zebrafish embryos and larvae are small enough to be cultured in microwell plates, making them suitable for high-throughput screening. At the same time, zebrafish is a vertebrate and is a ...

Claims

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Application Information

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IPC IPC(8): A61K49/00
Inventor 钱勇谢天培魏国志王新亮邓春来杨双明
Owner SHANGHAI BAINIAN SHIDANDE INSPECTION TECH
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