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Freshwater rotifer common nutrient solution and preparation method thereof

A technology for culture medium and rotifers, applied in animal husbandry and other directions, can solve the problems of high hardness, alkalinity or chloride degree of culture medium, imbalance of ion composition and proportion, and inability to apply freshwater rotifers, etc. Increased growth rate, wide-ranging effects

Inactive Publication Date: 2009-12-09
DALIAN FISHERIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But above two kinds of rotifer culture fluids all have the following problems: (one) because natural fresh water lacks buffer system, thereby the pH of culture fluid is extremely unstable, often occurs large-scale fluctuation; (two) natural fresh water The composition and ratio of ions are often unbalanced, and some ions are too many, such as Ca 2+ , making the hardness, alkalinity or chlorine of the culture medium high; (3) Environmental pollution often leads to high concentrations of toxic substances (biological toxins, heavy metals and organic substances, etc.) in natural fresh water, and these toxic substances are difficult to pass through aeration. (4) Due to the difference in the background value of environmental conditions, the application of natural fresh water in a certain area has limitations, and it cannot be applied to freshwater rotifers collected in other areas, and it is not universal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Prepare freshwater rotifer general culture solution as follows: first take 2.3ml 0.2mol / LNaH 2 PO 4 solution and 7.7ml 0.2mol / L Na 2 HPO 4 The solution was dissolved in 500ml of distilled water to prepare a phosphate buffer solution with a pH value of 7.3; then, 8.0ml of the following solutions were sequentially added to the phosphate buffer solution: 10.0g / l KNO 3 Solution - 6.2g / l MgSO 4 ·7H 2 O solution - 4.0 g / l of K 2 HPO 4 Solution - 14.4g / l Ca(NO 3 ) 2 ·6H 2 O solution, and finally dilute to 1000ml with distilled water, add 0.5μg of vitamin H and vitamin B to the 1000ml solution respectively 12 , which is prepared as a common culture medium for freshwater rotifers.

[0035] The test results are as follows:

[0036] 1. After using the rotifer culture solution configured in the present invention for 10 days, the survival rate and population growth rate of Brachionus calyciflora are shown in Table 1.

[0037] Table 1

[0038] Survival rate of...

Embodiment 2

[0047] According to the method described in Example 1, prepare freshwater rotifer general culture solution, in NaH 2 PO 4 and Na 2 HPO 4 Add following raw material in the phosphate buffer solution of pH 7.3 of preparation, each raw material amount is as follows in the final product:

[0048] KNO 3 10.0g / l

[0049] MgSO 4 ·7H 2 O 6.2g / l

[0050] K 2 HPO 4 4.0g / l

[0051] Ca(NO 3 ) 2 ·6H 2 O 14.4g / l

[0052] Vitamin H 0.5 μg / l

[0053] Vitamin B 12 0.5 μg / l.

Embodiment 3

[0055] According to the method described in Example 1, prepare freshwater rotifer general culture solution, in NaH 2 PO 4 and Na 2 HPO 4 Add following raw material in the phosphate buffer solution of pH 7.3 of preparation, each raw material amount is as follows in the final product:

[0056] KNO 3 6.0g / l

[0057] MgSO 4 ·7H 2 O 3.72g / l

[0058] K 2 HPO 4 2.4g / l

[0059] Ca(NO 3 ) 2 ·6H 2 O 8.64g / l

[0060] Vitamin H 0.5 μg / l

[0061] Vitamin B 12 0.5 μg / l.

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PUM

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Abstract

The invention relates to an organism nutrient solution, in particular to a freshwater rotifer common nutrient solution; the preparation method is that potassium nitrate, magnesium sulfate heptahydrate, calcium nitrate hexahydrate, dipotassium phosphate, vitamin B12 and vitamin H are added into phosphate buffer with pH of 7.0-7.5. The invention with different proportions is added to distilled water to prepare the rotifer nutrient solution which is applicable to the common types of freshwater rotifers, and the nutrient solution has high stability, simple preparation, no poison and no harm and wide applicable scope, provides conditions for the indoor culture of the model organism rotifer studied in experimental ecology, improves the indoor culture quality of the freshwater rotifer and reduces the risk of rotifer cultivation.

Description

technical field [0001] The invention relates to a biological culture solution, in particular to a general indoor freshwater rotifer culture solution. Background technique [0002] Certain species of freshwater rotifers, such as Brachionus calyciflorus, are internationally recognized model organisms that can provide basic experimental materials for indoor population ecology, molecular biology and ecotoxicology studies. Since rotifers are living test organisms, they need to be cultured in the laboratory for a long time in order to obtain a sufficient number of fresh individuals for experiments. The key technology of indoor cultivation of freshwater rotifers is exactly the culture medium of rotifers. Currently, most laboratories grow rotifers in aerated tap water or filtered field pond water. But above two kinds of rotifer culture fluids all have the following problems: (one) because natural fresh water lacks buffer system, thereby the pH of culture fluid is extremely unstabl...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 殷旭旺赵文刘钢魏杰王丽陈晓霞
Owner DALIAN FISHERIES UNIVERSITY
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