Method for carrying out toxicity analysis on tail water of sewage treatment plant by using caenorhabditis elegans

A technology for Caenorhabditis elegans and sewage treatment, applied in the direction of testing water, material inspection products, etc.

Inactive Publication Date: 2014-07-09
CHANGZHOU TEXTILE GARMENT INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the tail water discharged from the sewage treatment plant will pose a great challenge to the receiving water environment.

Method used

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  • Method for carrying out toxicity analysis on tail water of sewage treatment plant by using caenorhabditis elegans
  • Method for carrying out toxicity analysis on tail water of sewage treatment plant by using caenorhabditis elegans
  • Method for carrying out toxicity analysis on tail water of sewage treatment plant by using caenorhabditis elegans

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Embodiment 1

[0024] A method for analyzing the toxicity of tail water from a sewage treatment plant using Caenorhabditis elegans, the method comprises the following steps:

[0025] (1) Cultivation of Caenorhabditis elegans: use nematode medium NGM to cultivate Caenorhabditis elegans, feed on Escherichia coli OP50, and culture at 15°C;

[0026] (2) Cultivation of Escherichia coli OP50: use LB liquid medium for cultivation, and the temperature is controlled at 15°C;

[0027](3) Recovery of Caenorhabditis elegans: Take a 1.5ml centrifuge tube and add 0.5% OP50 solution respectively, put the centrifuge tube into the centrifuge, set r to 11000rpm, centrifuge for 0.5min, then take out the centrifuge tube and use Suck out 750 μl of the supernatant solution with a micropipette and discard it. The remaining concentrated OP50 is mixed with a pipette and transferred to the center of the NGM culture dish. Gently rotate the culture dish to spread the OP50 evenly in the culture dish. After the moss is ...

Embodiment 2

[0037] A method for analyzing the toxicity of tail water from a sewage treatment plant using Caenorhabditis elegans, the method comprises the following steps:

[0038] (1) Cultivation of Caenorhabditis elegans: use the nematode medium NGM to cultivate Caenorhabditis elegans, feed on Escherichia coli OP50, and culture at 20°C;

[0039] (2) Cultivation of Escherichia coli OP50: use LB liquid medium for cultivation, and the temperature is controlled at 20°C;

[0040] (3) Recovery of Caenorhabditis elegans: Take two 1.5ml centrifuge tubes and add 1ml of OP50 solution respectively, put the centrifuge tubes into the centrifuge, set r to 12000rpm, centrifuge for 1min, then take out the centrifuge tubes, and use a micropipette Suck out 750 μl of the supernatant solution with a pipette and discard. The remaining concentrated OP50 is mixed with a pipette and transferred to the center of the NGM culture dish. Gently rotate the culture dish to spread the OP50 evenly in the culture dish. ...

Embodiment 3

[0050] A method for analyzing the toxicity of tail water from a sewage treatment plant using Caenorhabditis elegans, the method comprises the following steps:

[0051] (1) Cultivation of Caenorhabditis elegans: use nematode medium NGM to cultivate Caenorhabditis elegans, feed on Escherichia coli OP50, and culture at 25°C;

[0052] (2) Cultivation of Escherichia coli OP50: use LB liquid medium for cultivation, and the temperature is controlled at 25°C;

[0053] (3) Recovery of Caenorhabditis elegans: Take four 1.5ml centrifuge tubes and add 1.5ml of OP50 solution respectively, put the centrifuge tubes into the centrifuge, set r to 13000rpm, centrifuge for 1.5min, then take out the centrifuge tubes, Use a micropipette to suck out 750 μl of the supernatant solution and discard it. The remaining concentrated OP50 is mixed with a pipette and transferred to the center of the NGM petri dish. Gently rotate the petri dish to spread the OP50 evenly in the petri dish. After the bacteria...

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Abstract

The invention discloses a method for carrying out toxicity analysis on tail water of a sewage treatment plant by using caenorhabditis elegans. The method comprises the following steps of (1) cultivation of the caenorhabditis elegans; (2) cultivation of escherichia coli OP 50; (3) anabiosis of the caenorhabditis elegans; (4) passage of caenorhabditis elegans; (5) synchronization of the caenorhabditis elegans; and (6) testing the comprehensive toxicity of a to-be-detected tail water sample by using the caenorhabditis elegans. The method provided by the invention has the beneficial effects that model organism-caenorhabditis elegans is utilized to carry out toxicity analysis on the tail water of the sewage treatment plant, and the size of the comprehensive toxicity of the waste water is judged by contrasting the difference of fatality rate, reproduction rate, motor behavior and generation cycle of nematodes and exposing nematodes of a blank control group.

Description

technical field [0001] The invention relates to the field of sewage toxicity detection, in particular to a method for analyzing the toxicity of tail water of a sewage treatment plant by using Caenorhabditis elegans. Background technique [0002] With the development of industry and the improvement of urbanization level, the discharge of sewage is increasing. At present, most of the urban sewage and industrial wastewater enter the sewage treatment plant for centralized treatment. The sewage treatment plant collects and treats the sewage in a unified manner. Except for a small part of the treated tail water, most of the tail water is discharged into the natural water body. . Therefore, the tail water discharged from the sewage treatment plant will pose a great challenge to the environment of the receiving water body. Contents of the invention [0003] The invention provides a method for analyzing the toxicity of the tail water of a sewage treatment plant by using the Cae...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/18
Inventor 曹侃壮子恒李敏薛婷杨蕴敏
Owner CHANGZHOU TEXTILE GARMENT INST
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