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Method for controlling microbes in algae powder by combination of microwave sterilization and spray drying

A technology of microorganisms and microalgae powder, which is applied in food drying, application, food preparation, etc., and can solve problems such as ineffective effects, poor applicability, and quality changes of algae powder

Inactive Publication Date: 2014-08-20
JIAXING ZEYUAN BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] In summary, if the algae powder is sterilized directly, although heat sterilization and radiation sterilization can make the microbial indicators meet the standard requirements, the above methods are likely to cause changes in the quality of the algae powder or radiation residues, and the applicability is poor; and It is expected that it is difficult or even impossible to control the introduction of miscellaneous bacteria during the large-scale photoautotrophic culture of algae or the operation process.
Spray drying itself is also a sterilization process, but the effect of spray drying alone is not obvious

Method used

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  • Method for controlling microbes in algae powder by combination of microwave sterilization and spray drying
  • Method for controlling microbes in algae powder by combination of microwave sterilization and spray drying
  • Method for controlling microbes in algae powder by combination of microwave sterilization and spray drying

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Experimental program
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Effect test

Embodiment 1

[0054] Embodiment 1: implement sterilization by existing technology

[0055] Proceed as follows:

[0056] (1) The "heterotrophic-dilution-light-induced" technology (see Chinese invention patent: ZL200610025618.9) is used to cultivate the chlorella algae liquid. After determination, the microbial indicators (converted to dry basis) are: the total number of colonies 1.5 × 10 10 CFU / g, coliform group ≤30MPN / 100g, mold 25CFU / g, yeast 20CFU / g, pathogenic bacteria (Salmonella, Staphylococcus aureus, Shigella, hemolytic streptococcus) were not detected;

[0057] (2) Concentrate by centrifugation (5000 rpm, 10 min), and the solid content of the concentrated solution is 14.5% (percentage by weight). After determination, the microbial indicators (converted to dry basis) are: the total number of colonies 1.35 × 10 10 CFU / g, coliform group ≤120MPN / 100g, mold 140CFU / g, yeast 130CFU / g, pathogenic bacteria (Salmonella, Staphylococcus aureus, Shigella, hemolytic streptococcus) were not det...

Embodiment 2

[0065] Embodiment 2: the sterilization implemented by technical scheme of the present invention

[0066](1) According to the "heterotrophic-dilution-light-induced" technology (see Chinese invention patent: ZL200610025618.9) to cultivate the chlorella algae liquid. After determination, the microbial indicators (converted to dry basis) are: the total number of colonies 1.5 × 10 10 CFU / g, coliform group ≤30MPN / 100g, mold 25CFU / g, yeast 20CFU / g, pathogenic bacteria (Salmonella, Staphylococcus aureus, Shigella, hemolytic streptococcus) were not detected;

[0067] (2) Collecting and concentrating the chlorella liquid by centrifugation, and the solid content of the concentrated solution is 14.5% (percentage by weight). After determination, the microbial indicators (converted to dry basis) are: the total number of colonies 1.35 × 10 10 CFU / g, coliform group ≤120MPN / 100g, mold 140CFU / g, yeast 130CFU / g, pathogenic bacteria (Salmonella, Staphylococcus aureus, Shigella, hemolytic strept...

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Abstract

The invention relates to a method for controlling microbe indexes in microalgae powder by combination of microwave sterilization and spray drying and a method for producing microalgae powder. The method comprises the following steps: carrying out microwave sterilization on a microalgae concentrated solution to obtain a sterilized concentrated solution, and drying the sterilized concentrated solution to obtain microalgae powder, thereby controlling the microbe indexes in the microalgae powder (including but not limited to total bacterial count, mold quantity and yeast quantity); and producing the microalgae powder. The method can effectively control the microbe indexes (especially total bacterial count) of the algae powder, and can maximally maintain the biological activity of the active substances in the microalgae. The method is suitable for but not limited to producing Chlorella, Haematococcus pluvialis, spirulina, Dunaliella, Nannochloropsis oculata, Schizochytrium, Crypthecodinium cohnii and various algae powders, and is hopeful to become a universal production method capable of effectively controlling microbe indexes (especially total bacterial count) in the algae powder.

Description

technical field [0001] The invention relates to a method for controlling microbial indicators in microalgae powder, especially the total number of colonies, in particular to controlling the microbial indicators in microalgae powder, especially the total number of colonies, by coupling microwave sterilization and spray drying, and performing microalgae powder production. Background technique [0002] Microalgae grow rapidly, and the cells are rich in protein, β-carotene, astaxanthin, unsaturated fatty acids (DHA, RHA) and various trace elements, etc., which have great economic development value. [0003] The crude protein content of microalgae can reach more than 60%, and some microalgae have been developed into health products, food additives, etc.; feeding pets with microalgae can make their hair shiny or feathers more beautiful; using microalgae to feed zooplankton, It also has broad development potential in aquaculture (O.Pulz, W.Gross. Valuable products from biotechnolo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/015A23L1/025A23L5/20A23L5/30
CPCA23L5/34A23L5/21A23V2002/00A23V2300/10A23V2300/24
Inventor 李元广张林孙炳耀沈国敏王军潘荣华徐新华范建华
Owner JIAXING ZEYUAN BIOLOGICAL PROD
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