Method for controlling microbes in algae powder by combination of pasteurization and spray drying

A microbial and pasteurization technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as insignificant effects, poor applicability, and changes in the quality of algal flour

Inactive Publication Date: 2014-08-20
JIAXING ZEYUAN BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] In summary, if the algae powder is sterilized directly, although heat sterilization and radiation sterilization can make the microbial indicators meet the standard requirements, the above methods are likely to cause changes in the quality of the algae powder or radiation residues, and the applicability is poor; and It is not feasible to expect to control the introduction of bacteria during algae cultivation or operation
Spray drying itself is also a sterilization process, but the effect of spray drying alone is not obvious

Method used

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  • Method for controlling microbes in algae powder by combination of pasteurization and spray drying
  • Method for controlling microbes in algae powder by combination of pasteurization and spray drying
  • Method for controlling microbes in algae powder by combination of pasteurization and spray drying

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Experimental program
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Effect test

Embodiment 1

[0058] Embodiment 1: implement sterilization by existing technology

[0059] Proceed as follows:

[0060] (1) Adopt the "heterotrophic-dilution-light-induced" technology (refer to Chinese invention patent: ZL200610025618.9) to cultivate and obtain the chlorella algae liquid. After measurement, the microbial indicators (converted to dry basis) are: the total number of colonies 2.5× 10 10 CFU / g, coliform group ≤30MPN / 100g, mold 20CFU / g, yeast 15CFU / g, pathogenic bacteria (Salmonella, Staphylococcus aureus, Shigella, hemolytic streptococcus) were not detected;

[0061] (2) by centrifugal concentration (5000rpm, 10min), the concentrated solid content of chlorella after concentration is 14.3% (percentage by weight), after measuring, microbial index (converted to dry basis) is respectively: total number of colonies 2.3 * 10 10 CFU / g, coliform group ≤120MPN / 100g, mold 130CFU / g, yeast 100CFU / g, pathogenic bacteria (Salmonella, Staphylococcus aureus, Shigella, hemolytic streptococcus...

Embodiment 2

[0069] Embodiment 2: the sterilization implemented by technical scheme of the present invention

[0070] (1) According to the "heterotrophic-dilution-light-induced" technology to cultivate the chlorella algae liquid (see Chinese invention patent: ZL200610025618.9), after determination, the microbial indicators (converted to dry basis) are: the total number of colonies 2.5 × 10 10 CFU / g, coliform group ≤30MPN / 100g, mold 20CFU / g, yeast 15CFU / g, pathogenic bacteria (Salmonella, Staphylococcus aureus, Shigella, hemolytic streptococcus) were not detected;

[0071] (2) Collect and concentrate the chlorella algae liquid by centrifugation (5000rpm, 10min), the concentrated solid content of chlorella after concentration is 14.3% (percentage by weight), after measuring, the microbial index (converted to dry basis) is respectively : Total number of colonies 2.3×10 10 CFU / g, coliform ≤120MPN / 100g, mold 130CFU / g, yeast 100CFU / g, pathogenic bacteria (Salmonella, Staphylococcus aureus, Shig...

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Abstract

The invention relates to a method for controlling microbe indexes in microalgae powder by combination of pasteurization and spray drying and a method for producing microalgae powder. The method comprises the following steps: pasteurizing a microalgae concentrated solution to obtain a sterilized concentrated solution, and drying the sterilized concentrated solution to obtain microalgae powder, thereby controlling the microbe indexes in the microalgae powder (including but not limited to total bacterial count, mold quantity and yeast quantity); and producing the microalgae powder. The method can effectively control the microbe indexes (especially total bacterial count) of the algae powder, and can maximally maintain the activity of the active substances in the microalgae. The method is suitable for but not limited to producing Chlorella, Haematococcus pluvialis, spirulina, Dunaliella, Nannochloropsis oculata, Schizochytrium, Crypthecodinium cohnii and various algae powders, and is hopeful to become a universal production method capable of effectively controlling microbe indexes (especially total bacterial count) in the algae powder.

Description

technical field [0001] The invention relates to a method for controlling microbial indicators in microalgae powder, especially the total number of colonies, in particular to a method for controlling microbial indicators in microalgae powder, especially the total number of colonies, by coupling pasteurization and spray drying. production. Background technique [0002] Microalgae grow rapidly, and the cells are rich in protein, β-carotene, astaxanthin, unsaturated fatty acids (DHA, RHA) and various trace elements, etc., which have great economic development value. [0003] The crude protein content of microalgae can reach more than 60%, and some microalgae have been developed into health products, food additives, etc.; feeding pets with microalgae can make their hair shiny or feathers more beautiful; using microalgae to feed zooplankton, It also has broad development potential in aquaculture (O.Pulz, W.Gross. Valuable products from biotechnology of microalgae. Appl Microbiol ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L2/04C12N1/12C12R1/89
Inventor 李元广张林孙炳耀沈国敏王军潘荣华徐新华范建华
Owner JIAXING ZEYUAN BIOLOGICAL PROD
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