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Production method and application of aquatic feed

A production method and feed technology, applied in the fields of application, animal feed, animal feed, etc., can solve the problems of insignificant effect, unflavored flavor, and food-attractive effect reports

Active Publication Date: 2017-03-01
益海嘉里(防城港)生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Jiao Jiangang, Yuan Kathy et al. (Journal of Shanghai Ocean University, Volume 23, Phase 4) studied its effect on the growth performance and muscle nutritional components of Penaeus vannamei by adding a certain amount of Schizochytrium fermented powder in the feed. Show: when adding amount reaches 0.5% of basal feed weight, can improve individual weight gain and reduce feed coefficient, but weight gain rate, survival rate and feed coefficient have no significant influence; The effect is not obvious
Menghe H.Li et al. (Aquaculture, 2009, Volume 292, 232-236) added dry powder of Schizochytrium algae to the feed of channel catfish. When the addition amount was 1.0% or 1.5% of the feed weight, the weight gain effect was obvious, but No report on its flavor and food-attractive effect

Method used

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  • Production method and application of aquatic feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1: strain activation

[0059] Activation of Bacillus subtilis (Bacillus subtilis): pick a ring of Bacillus subtilis B3 glycerol tube (preservation number: CGMCC No.7641), streak it on an LB plate, and culture overnight in a 37°C incubator;

[0060] Activation of Schizochytrium (Schizochytrium.ATCC20888) (ATCC20888, purchased from Guangzhou Institute of Microbiology): pick a ring of Schizochytrium glycerol tube, streak on the seed basal medium plate, and culture in a 28°C incubator for 2 sky.

[0061] Seed basal medium: glucose 100g / L, yeast extract 15g / L, (NH4) 2 SO 4 1g / L, KH 2 PO 4 3g / L, Na 2 SO 4 12g / L, MgSO 4 5g / L, K 2 SO 4 7g / L, KCl 2g / L.

Embodiment 2

[0062] Embodiment 2: Seed solution preparation and plate co-cultivation

[0063] Preparation of Bacillus subtilis B3 seed solution: pick a single colony from the activated plate, inoculate it in 50ml medium for shake flask culture, the fermentation temperature is 37°C, the pH value is 6.8, the rotation speed is 200rpm, and the fermentation time is 18h ; The concentration of Bacillus subtilis seed solution is: OD after 10-fold dilution 600nm = 0.8. Shake flask fermentation medium is: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L.

[0064] Preparation of Schizochytrium sp. seed solution: Pick a single colony from the activated plate, inoculate it in 50ml medium for shake flask culture, the fermentation temperature is 28°C, the pH value is 6.8, and the rotation speed is 200rpm , the fermentation time is 72h; the concentration of Schizochytrium seed solution is: after 10-fold dilution, the bacterial concentration reaches OD 600nm = 0.8. The shake flask fermentation...

Embodiment 3

[0066] Embodiment 3: solid state co-cultivation-I

[0067] After the soybean meal was sterilized at 90°C for 30 minutes, it was mixed with sterile water so that the initial water content of the soybean meal was 50%. Based on the total weight of the soybean meal containing water, the inoculation amount of 10% by volume to weight was added to Example 2 The prepared mixed solution (1:1) of Bacillus subtilis and Schizochytrium seed solution was sealed with 4 layers of gauze, and fermented in a constant temperature and humidity chamber at 37° C. and a humidity of 90% for 48 hours. The results of the fermentation are listed in Table 1 below.

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Abstract

The invention relates to a production method and application of an aquatic feed. Specifically, the invention relates to a preparation method for dimethylpropiothetin, a production method for a feed or a method for improving the flavor of fermented soybean meal. The method is characterized by comprising a step of fermenting soybean meal by using bacillus microbes and Schizochytrium. The invention also provides a fermented soybean meal which is prepared through fermentation via the bacillus microbes and Schizochytrium according to the method. The invention further provides a feed containing the fermented soybean meal. As the method is used for fermentation of soybean meal, anti-nutrition factors in the soybean meal are degraded, the content of small peptide is increased, and the produced fermented soybean meal has special flavor and can increase the food intake and weight gain rate of aquatic animals, reduce a feed coefficient and improve breeding benefits.

Description

technical field [0001] The invention relates to a production method and application of aquatic feed. Specifically, the present invention relates to a method for producing aquatic feed by co-culture of Bacillus subtilis and Schizochytrium sp. and its application. Background technique [0002] Food attractant is an important component of aquatic compound feed. Appropriate food attractant can enhance aquatic animals' preference for artificially formulated feed, increase food intake, and promote growth, thereby improving breeding efficiency and greatly reducing the pollution of aquaculture water. [0003] There are many kinds of attractants, which can be divided into visual attractants, taste attractants and smell attractants according to the mechanism of action; for aquatic animals, there are many studies on smell and taste attractants, and the sense of smell of aquatic animals is relatively sensitive , the lower concentration of chemical substances in the water body can be fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K20/105A23K50/80
CPCY02A40/818
Inventor 柴丹张敏宋鹏
Owner 益海嘉里(防城港)生物科技有限公司
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