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Fermentation production technique of schizogenesis chytrid

A technology of Schizochytrium and fermentation tank, which is applied in the field of microbial fermentation production, and can solve the problems of high production cost and low output

Inactive Publication Date: 2012-06-20
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a fermentation production process of Schizochytrium, which can solve the problems of low output and high production cost in the prior art

Method used

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  • Fermentation production technique of schizogenesis chytrid
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  • Fermentation production technique of schizogenesis chytrid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Effects of Compound Nitrogen Source Medium on Biomass and DHA Content of Schizochytrium

[0038] Preparation of soybean meal hydrolyzate (converted into soybean meal weight) with a concentration of 40g / L

[0039] a. Add 82mL of hydrochloric acid with a concentration of 1mol / L to 40g of soybean meal, and add 231mL of distilled water.

[0040] b. 121°C, high temperature hydrolysis for 30 minutes.

[0041] c. Add 1mol / L NaOH to adjust the pH to 7.0, centrifuge, and keep the supernatant.

[0042] d. Add distilled water to dilute the supernatant to 1 L, and the obtained solution is a soybean meal hydrolyzate with a concentration of 40 g / L (converted to the weight of soybean meal).

[0043] e. If other concentration of soybean meal hydrolyzate is needed, just adjust the dosage of soybean meal accordingly.

[0044] In a 250ml Erlenmeyer shaker flask, add 25ml natural seawater and 25ml distilled water respectively, add carbon source (glucose) and nitrogen source (soybean mea...

Embodiment 2

[0049] Effects of Different Soybean Meal Hydrolysis Processes on the Biomass and DHA Content of Schizochytrium

[0050] After the soybean meal hydrolyzate is prepared in the following way, add 600L medium to a 1000L fermenter, adjust the pH value to 6.0, and add vitamins B1, B12, H. After sterilization, insert the bacteria seed solution and cultivate at 25°C 4 days. Collect the cells by centrifugation and measure their dry weight; 3 -Methyl esterification by methanol method, the percentage content of DHA in the bacteria is determined by gas chromatography. The results are shown in Table 2.

[0051] Hydrolysis scheme

[0052] A Add soybean meal powder and appropriate amount of water into the enamel reaction kettle, add 30% concentrated hydrochloric acid to adjust the pH of the solution to 1.3. Steam is heated to 110°C and kept for 30 minutes. After cooling, the pH is adjusted to 6.0. The residue is separated from the solution, and the obtained supernatant is used as the cul...

Embodiment 3

[0061] Effects of Different Hydrolysis Conditions on the Biomass and DHA Content of Schizochytrium

[0062] After determining the hydrolysis scheme, the hydrolysis time (min), hydrolysis temperature (°C), and acid concentration (M) were used for orthogonal experiment optimization; the experimental gradient was hydrolysis time 15min, 20min, 25min, hydrolysis temperature 100°C, 110°C, 120°C, hydrochloric acid concentration 0.25mol / L, 0.4mol / L, 1.0mol / L. Add 600L medium to a 1000L fermenter, adjust the pH value to 6.0, and add vitamins. After sterilization, insert the bacterial seed solution and cultivate at 25°C for 4 days. Collect the cells by centrifugation and measure their dry weight; 3 -Methyl esterification by methanol method, the percentage content of DHA in the bacteria is determined by gas chromatography. The results are shown in Table 3.

[0063] Table 3 Orthogonal experiment table for optimization of hydrolysis conditions

[0064] experiment

[0065] Usi...

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Abstract

The invention provides a fermentation production technique of schizogenesis chytrid, which solves the problems of lower yield and higher production cost in the prior art. The fermentation production technique has the technical scheme that bean pulp is taken as main nitrogen source for fermentation production, and the technique comprises the steps of bean pulp hydrolysis, pressing filter, preparation of fermentation medium, arrangement of a fermentation tank system, collection and drying of the schizogenesis chytrid, and preparing the schizogenesis chytrid dry powder. The new fermentation production technique of the schizogenesis chytrid takes the bean pulp as main nitrogen, thus not only reducing the cost of fermentation production, but also improving the growth rate of the schizogenesis chytrid and promoting fatty acid accumulation of the cells of the schizogenesis chytrid.

Description

technical field [0001] The invention belongs to the field of microbial fermentation production, and in particular relates to a fermentation production process of Schizochytrium with soybean meal as the main nitrogen source. Background technique [0002] In recent years, the important role of polyunsaturated fatty acids (PUFAs) on human health has been paid more and more attention. Among them, docosahexaenoic acid (22:6, n-3, docosahexaenoic acid, DHA), one of the important ω-3 PUFAs, is favored because of its important physiological functions in humans and animals. Studies have shown that DHA is the basic component of cell membranes in some tissues of the human body, plays an important role in the development of the baby's visual system and nervous system, and also has pharmacological effects such as lowering cholesterol, anticoagulation, and preventing cancer. In addition, DHA is also an essential fatty acid required for the growth and development of various seawater fish,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/645
Inventor 张学成宋晓金臧晓南
Owner OCEAN UNIV OF CHINA
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