50 results about "IMMUNE ENHANCEMENT AGENTS" patented technology

The preparation method comprises the following steps: selecting PLGA (poly (lactic-co-glycolic acid)) with biocompatibility and biodegradability as a carrier material, loading an immunopotentiator alpha-tocopherol on PLGA nano-particles, and preparing the nano-particle immunologic adjuvant by using a nano-precipitation method, so as to obtain the nano-particle immunologic adjuvant with an immune synergistic effect. By controlling the polymer concentration and the drug concentration in the preparation process, the average particle size of the drug-loaded nano-particles is 90-120nm, the monodispersion coefficient is less than 0.2, the particle size of the product is uniform and stable, the encapsulation efficiency reaches 80% or more, the content of alpha-tocopherol in the nano-particles is 1-30%, and the drug-loaded nano-particles have a sustained-release effect of one month or more. The PLGA nanoparticles loaded with alpha-tocopherol can promote proliferation of mouse macrophages and enhance secretion of cell inflammatory cytokines and expression of surface costimulatory molecules, have a synergistic enhancement effect of the PLGA nanoparticles and alpha-tocopherol, and can be used as an effective immunologic adjuvant.

The invention relates to the technical field of aquatic animal immune regulation and control, in particular to rHoLL-like stepped lectin-like recombinant protein as well as a preparation method and application thereof. The recombinant protein has pathogen-related molecular pattern recognition and combination capabilities, can promote aggregation of pathogens in a body fluid environment, and activates an aquatic animal complement system, so that the non-specific immune defense capability is enhanced. The preparation method comprises the following steps: amplifying a HoLL-like mature peptide coding sequence, constructing a recombinant expression vector, and converting the recombinant expression vector into an escherichia coli expression host for induced expression to obtain rHoLL-like lectin-like recombinant protein existing in an inclusion body form; and carrying out affinity chromatography purification on the inclusion body protein, and carrying out renaturation treatment in a manner of gradient reduction of denaturant concentration to obtain the rHoLL-like stepped lectin-like recombinant protein. The rHoLL-like stepped lectin-like recombinant protein has good biological safety, and can be applied to aquaculture as an aquatic animal immunopotentiator or a related biological product.

The application belongs to the technical field of molecular biology, and particularly relates to a long oyster C-type lectin CgCLec-TM2 functional domain CRD recombinant protein and application. The amino acid sequence of the long oyster C-type lectin CgCLec-TM2 functional domain CRD recombinant protein is shown as SEQ ID NO. 1. The rCRD obtained by the application has binding capacity to various pathogen-associated molecular patterns (PAMPs), can bind and agglutinate various microorganisms, has a growth inhibiting effect on gram-negative bacteria Vibrio splendidus and Escherichia coli, and can be used for the development of bacteriostatic feed additives and new-type immune enhancers.

The invention discloses a compound immunopotentiator and application thereof in prevention and control of aquatic diseases, and belongs to the technical field of aquaculture. The compound immunopotentiator is prepared by scientifically compounding eucommia ulmoides, astragalus membranaceus, curcumin, vitamin A, vitamin D3, vitamin E and vitamin K3. According to the compound immunopotentiator, through the synergistic effect of the Chinese herbal medicine extract and the compound vitamins, the autoimmunity of aquaculture animal bodies can be remarkably improved, the resistance of the aquaculture animal bodies to pathogenic microorganisms and bad environments is enhanced, the compound immunopotentiator further has a good pathogen removing effect, and damage of pathogens to the aquaculture animals can be effectively reduced. The compound immunopotentiator provided by the invention is used as a combination of a plant-derived drug and multivitamins, has no problems of environmental pollution and drug resistance, and is good in safety and effectiveness.

The application provides a turbot compound immune enhancer, a preparation method and application thereof. The application discloses application of a composition containing a series of components in preparation of a turbot immune enhancer, and the immune enhancer can effectively improve expression of a proinflammatory related gene of the turbot, reduce a number of bacteria in a liver or an intestinal tract, enhance a bactericidal capacity of the turbot, enhance an antioxidant stress capacity of the turbot, relieve liver damage of the turbot after being infected by bacteria, and relieve tissue damage of the turbot after being infected by bacteria. The immune enhancer component is simple, environment-friendly, low in cost and ideal in effect, and provides a new scheme for improving a breeding level of marine fish and improving a survival rate.

The application provides a low-molecular-weight high-purity Stropharia rugosannulata glycoprotein and a preparation method and application thereof, and belongs to the technical field of biochemistry. The Stropharia rugosannulata glycoprotein has a molecular weight of 10-20 kDa, the protein content of the Stropharia rugosannulata glycoprotein is 75%-82%, and the polysaccharide content is 10%-18%; the protein part of the Stropharia rugosannulata glycoprotein contains 17 kinds of amino acids, i.e., glycine, alanine, aspartic acid, threonine, serine, glutamic acid, cysteine, valine and methionine; and the polysaccharide part of the Stropharia rugosannulata glycoprotein mainly includes fucose, rhamnose, arabinose, galactose, glucosamine, glucose, xylose and mannose. The Stropharia rugosannulata glycoprotein prepared by the application has a purity of more than 95% and stable quality, has an immune enhancement effect, and can be used as an immune enhancer raw material for the development of functional food, health products and medicines.

This invention discloses a method, apparatus, equipment, and storage medium for the synergistic blocking of harmful substances in chicken houses. The method includes collecting environmental parameters, physiological states of the flock, and pathogen information from the chicken house; sending the collected information to an intelligent decision-making system; and when parameters exceed thresholds or pathogenic agents are detected, the intelligent decision-making system generates a synergistic control strategy and triggers a coordinated response: a light disinfection system activates a specific wavelength light source to form an effective disinfection area; a ventilation system guides airflow into the effective disinfection area; a drinking water disinfection system adds effective disinfectants and immune enhancers to the drinking water; and the intelligent decision-making system dynamically adjusts the parameters. In this embodiment, the light disinfection system, ventilation system, and drinking water disinfection system not only achieve the effect of a single disinfection method, but also work together to significantly reduce the risk of highly contagious diseases in the flock, ensuring the normal growth of the chickens and avoiding economic losses caused by diseases.

The present invention provides an application of recombinant chicken thymopeptide in the preparation of chicken immune enhancers, belonging to the field of veterinary technology. In the present invention, chicken thymosin α1, chicken thymosin α1 mutant and double-copy tandem protein of chicken thymosin α1 mutant are expressed by genetically engineered recombinant Escherichia coli. The action time of recombinant chicken thymopeptide in chickens is increased through two ways: mutating the positively charged amino acid K into the negatively charged amino acid D and double-copy tandem expression. Seven weeks after the injection of thymopeptide, the SI value of the chicken thymosin α1 group has dropped to 1.08, approaching the control group level, the SI value of the chicken thymosin α1 mutant group has dropped to 1.23, while the SI value of the double-copy chicken thymosin α1 mutant group still maintains a relatively high level of 1.43. It can be seen that the double-copy chicken thymosin α1 mutant has a more persistent effect on regulating the immune function of chickens than chicken thymosin α1 expressed by the natural sequence, and is a long-acting thymopeptide that can be used as an immune enhancer.

The invention belongs to the technical field of food fermentation and functional drinks, and particularly relates to lactobacillus mucilaginosus and application thereof in preparation of fermented wolfberry juice for improving immunity. The lactobacillus mucus LFY21 strain is preserved in the China Center for Type Culture Collection on November 17, 2025, and the preservation number of the lactobacillus mucus LFY21 strain is CCTCC NO: M 20252569; the preservation address is Wuhan University. The strain grows vigorously in the Chinese wolfberry juice, the acid production capacity is weak in the Chinese wolfberry juice fermentation process, and the obtained Chinese wolfberry juice is high in saccharic acid content, good in flavor and palatability and high in free radical scavenging activity and total phenol content. In addition, animal experiments prove that the fermented Chinese wolfberry juice prepared from the strain can also generate the effect of effectively improving the immune function, and has the application potential as an immunopotentiator.

The invention relates to a Gleditsia sinensis seed ethanol extract and a preparation method and application thereof in aquatic animal immunization, the preparation method of the Gleditsia sinensis seed ethanol extract comprises the following steps: crushing Gleditsia sinensis seeds, adding 75-80% ethanol solution according to a solid-to-liquid ratio of 1: 8-1: 10, and extracting; during extraction, heating is performed to 80-85 DEG C, reflux extraction is performed 2-3 times, and each time lasts for 1.5-2 hours; combining all extracting solutions, and filtering under reduced pressure to obtain clear filtrate; and concentrating the filtrate to 1 / 5 of the original volume under reduced pressure by using a rotary evaporator, and freeze-drying the concentrated solution to obtain faint yellow powder. The gleditsia sinensis seed ethanol extract is applied to improvement of the immune function of aquatic animals. The invention provides a novel immunopotentiator, namely the gleditsia sinensis seed extract, for aquaculture. The immunopotentiator is low in cost, simple in preparation process, remarkable in immunopotentiation effect, safe, environment-friendly and suitable for being mixed with feed for feeding aquatic animals.

This invention relates to the field of extracts, specifically to a method for preparing a traditional Chinese medicine extract and its application. The method includes pulverizing and sieving a traditional Chinese medicine formula, preparing a compound enzymatic hydrolysate using a compound enzyme, mixing the powdered medicine with the hydrolysate, performing enzymatic hydrolysis, ultrasonic extraction, fermentation, filtration, and freeze-drying to obtain the traditional Chinese medicine extract. The extract is used as an immune enhancer for animals. Existing methods for preparing traditional Chinese medicine extracts suffer from low extraction efficiency and poor drug efficacy. The method for preparing a traditional Chinese medicine extract and its application provided by this invention offers higher extraction efficiency and better drug efficacy.

The invention discloses a fish feed capable of improving disease resistance of fish, the fish feed comprises main materials, the main materials comprise a fish essence extract, a quality protein source and an immunopotentiator; the auxiliary materials mainly comprise starch and essence; the feed comprises a fish essence extract and an additive, the additive is used for keeping freshness and nutritional ingredients of the feed, the fish essence extract comprises a bean mixed extract and compound nutrients, the bean mixed extract comprises a soybean aqueous extract and a broad bean aqueous extract, the nutrients comprise beta-glucan, nucleic acid and vitamin C, and the compound nutrients comprise beta-glucan, nucleic acid and vitamin C. The bean mixed extract is prepared according to the grade of a finished product, and relates to the technical field of fish feed. The immunopotentiator comprises the acidic polysaccharide, the probiotics and the plant extract, and the content of the immunopotentiator in the high-grade finished product material, the content of the immunopotentiator in the high-grade finished product material and the content of the immunopotentiator in the general finished product material are the same, so that the disease resistance of different grades of fish feeds can be guaranteed, and the economic benefit of the fish feed can be effectively improved by performing graded manufacturing on the fish feed. The fish feed is convenient to sell.

The invention relates to a compound atomized liquid for pet respiratory mucosa and a preparation method thereof. Each 100 mL of the compound atomized liquid is prepared from 0.15 to 0.20 g of a liquorice root extract, 0.18 to 0.25 g of L-lysine hydrochloride, 0.20 to 0.25 g of chitosan oligosaccharide, 0.25 to 0.35 g of yeast-beta-glucan, 0.008 to 0.015 g of EDTA-2Na (Ethylene Diamine Tetraacetic Acid), 0.4 to 0.6 g of trehalose, 0.65 to 0.75 g of sodium chloride, 0.5 to 1.0 mg of micromolecular antimicrobial peptide and the balance of citric acid / phosphate buffer solution. Wherein the molecular weight of the chitosan oligosaccharide is less than or equal to 2000Da; the pH value of the compound atomized liquid is 6.0-7.0; and the osmotic pressure of the compound atomized liquid is 280-320 mOsm / kg. By compounding the natural anti-inflammatory component, the immunopotentiator, the mucosa protective agent and the micromolecular antimicrobial peptide, synergistic treatment is realized, and side effects are reduced.

The invention discloses a vigna unguiculata pest and disease damage biosafety prevention and control method based on screening and identification technology research. The method comprises the following steps: S1, identifying and selecting vigna unguiculata disease-resistant varieties; s2, testing and comparing the treatment effects of different disease-resistant agents on the cowpeas, and selecting the disease-resistant agent with the best effect; s3, treating the seeds before sowing; s4, soil insect prevention and control; s5, soil-borne diseases and root diseases are prevented and controlled; s6, preventing and treating leaf diseases; s7, preventing and controlling pests on the ground; s8, using an immunopotentiator; and S9, field cleaning. According to the method, disease-resistant varieties are identified and screened in the early stage of sowing, a new variety of root rot resistant cowpeas is bred and screened out, diseases are reduced from the source, and the prevention and treatment effects of biopesticide on main diseases and insect pests in different growth periods of cowpeas are systematically studied; the green comprehensive prevention and control technology with biopesticide as the main part and chemical pesticide synergistic prevention and control as the core is specifically used, economic and ecological benefits are considered, and green and safe production of cowpeas is guaranteed.

The present disclosure is generally directed to an immunogenic protein conjugate comprising anthrax toxin protective antigen (PA) protein as an immune enhancer, a peptide linker, and an antigen derived from an infectious or pathogenic virus or microorganism. This protein conjugate can be used to induce an immune response in a subject, such as in a vaccine against the antigen of the protein conjugate. For example, the antigen can be Spy0469 for a vaccine against S. pyogenes.

This invention discloses a triple inactivated vaccine containing Mycoplasma gallisepticum, Mycoplasma synoviae, and Infectious Laryngotracheitis in chickens, its preparation method, and its application. The technical solution includes inactivated Mycoplasma gallisepticum antigen, inactivated Mycoplasma synoviae antigen, and inactivated Infectious Laryngotracheitis Virus gD protein; wherein the inactivated Mycoplasma gallisepticum antigen is prepared from Mycoplasma gallisepticum strain YBMG1, and the preservation number of Mycoplasma gallisepticum strain YBMG1 is CCTCC M 20261107. This invention rationally combines Mycoplasma gallisepticum antigen, Mycoplasma synoviae antigen, and chicken infectious laryngotracheitis virus gD protein, and uses saponin as an immune enhancer. This effectively reduces the interference of chicken infectious laryngotracheitis virus gD protein on the immune response of Mycoplasma gallisepticum and Mycoplasma synoviae, and the effect is significantly better than other polysaccharide or polypeptide immune enhancers, thereby achieving simultaneous prevention and control of Mycoplasma gallisepticum disease, Mycoplasma synoviae disease, and chicken infectious laryngotracheitis.

The invention discloses a method for increasing the yield of extracellular vesicles of lactococcus lactis and application of the method, and belongs to the technical field of biology. The lysozyme and the ampicillin are used for weakening a lactococcus lactis peptidoglycan layer, so that the yield of the natural bacterial extracellular vesicles of the lactococcus lactis is increased. The technological process is simple, and is suitable for industrial amplification and standardized production; the vesicle yield is high, the amplification effect and the purification effect are good, and the method can be used for large-scale preparation of vesicles. The lactococcus lactis extracellular vesicles have the adjuvant efficacy of stimulating the maturation of dendritic cells and the tropism of targeting solid tumor enrichment, and can be used as an immunopotentiator or a sending carrier of vaccines and nano-drugs.

The application belongs to the technical field of aquaculture, and particularly relates to a method for improving the attachment rate and disease resistance of long oyster (Crassostrea gigas) nauplii and application thereof. The method is that early nauplii of the long oyster are soaked in filtered seawater containing a certain dose of glutathione. Compared with a control group without adding glutathione, the growth and development speed of the long oyster nauplii soaked in the glutathione is accelerated, the attachment rate is significantly improved, the lysozyme activity is enhanced, and the ability to resist vibrio is significantly increased. The application has a significant effect on reducing the mortality of the long oyster nauplii in the attachment metamorphosis process and improving the attachment metamorphosis rate, and can be used as an immune enhancer for the attachment metamorphosis stage of the long oyster and applied to seed production.

The invention belongs to the technical field of aquaculture, and discloses an immunopotentiator for aquaculture and application of the immunopotentiator. The immunopotentiator for aquaculture is prepared from the following components in parts by weight: 50 to 60 parts of ginsenoside, 15 to 20 parts of organic saccharicterpenin, 6 to 10 parts of a nutritional supplement, 1 to 5 parts of an immunopotentiating aid, 0.5 to 2 parts of an anti-stress aid and 0.2 to 1 part of an antioxidant. The ginsenoside and the organic saccharicterpenin which are naturally extracted are used as core active ingredients, safe auxiliaries are used as auxiliary materials, harmful ingredients such as antibiotics and hormones do not exist, medicine residues cannot be generated after the composition is used, and safety is high; meanwhile, through scientific compounding, all the components play a synergistic role, the non-specific immunity and anti-stress ability of aquatic animals can be improved, intestinal microecological balance can be improved, growth can be promoted, the culture water environment can be indirectly improved, and multiple effects are achieved.

The invention provides a polymer composite adjuvant for an animal vaccine and a preparation method thereof, and the polymer composite adjuvant for the animal vaccine comprises acrylate hydrogel, nano oil emulsion and an immunopotentiator, the acrylate hydrogel is prepared by the following steps: generating a polyacrylate precipitate from an acrylic hydrophilic monomer and an acrylic hydrophobic monomer in a solvent by adopting a precipitation polymerization method, and immersing the precipitate in ultrapure water, the hydrophilic monomer is one of acrylic acid and methacrylic acid, and the hydrophobic monomer is acrylate with the carbon chain length of 10-22; the immunopotentiator is a TLR3 (toll-like receptor 3) or TLR9 (toll-like The polymer composite adjuvant disclosed by the invention has good biocompatibility, low mineral oil content and small side effects, not only can play an antigen slow release role, but also can improve the cellular immune response of an organism, has the average particle size of 40-60 nm, and is good in dispersity, and a prepared vaccine is low in viscosity and easy to inject; and the preparation process is simple, the stability is high, and the biological safety is good.

The invention discloses a preparation method and application of a cell lysate gel for treating oral ulcers, and relates to the technical field of oral care. The cell lysate gel is prepared by mixing a functional solution and thrombin, the functional solution comprises a solvent and a solute, the solvent is a mesenchymal stem cell lysate, the solute comprises bovine fibrinogen, borneol, vitamins, polysaccharides, an immunopotentiator, chlorogenic acid, indomethacin, and ginkgo biloba, the vitamins comprise vitamin E and vitamin B2, the polysaccharides comprise astragalus polysaccharides and cuttlebone polysaccharides, the immunopotentiator comprises alpha-mannan peptide and total glucosides of white paeony. The invention avoids the side effects of mesenchymal stem cells, has the advantages of high fluidity, simple and convenient preparation, safety and environmental protection, good application treatment effect, and the like, and achieves remarkable results in the aspects of oral ulcer anti-inflammatory effect, oral ulcer angiogenesis, and enhancing rat immunity, thereby reflecting the great prospect of oral ulcer treatment.

The present application relates to the technical field of aquaculture fish vaccine, in particular to a grass carp NPM1a and type II reovirus VP38 fusion gene, recombinant protein, composition and application. The nucleotide sequence of the fusion gene is shown as SEQ ID NO: 1. The fusion gene is obtained by connecting grass carp NPM1a and type II reovirus VP38 through a linker sequence, a BamH restriction site is added at the 5' end of the fusion sequence, and a XhoI restriction site is added at the 3' end. The linker sequence is shown as SEQ ID NO: 2. By preparing a subunit vaccine of grass carp NPM1a and type II reovirus VP38 fusion protein, and jointly using indole-3-lactic acid as an immune enhancer, the technical bottlenecks of low protection rate of existing vaccines and poor intestinal mucosal barrier repair are overcome.

To provide an agonist anti CD40 antibody.SOLUTION: The application generally relates to identification of a specific agonist anti CD40 antibody. Based on the identification, the invention provides a novel agonist antibody, and use of the agonist antibody in therapy. The agonist anti CD40 antibody is suitable for use in various activities including coupling to CD40+DC and macrophage however not limited thereto. The antibody is combined with other immunopotentiators (for example, topical or systemic IL-2, TLR-7 agonists and / or cytotoxicity chemotherapy), therefore occurrence of strong anti-tumor CD8+ cytotoxicity T lymphocyte response can be detected.SELECTED DRAWING: None

The invention discloses a chicken anti-stress immunopotentiator in a high-temperature and high-humidity state. The chicken anti-stress immunopotentiator is prepared from the following raw materials in parts by weight: 40-55 parts of pine needles, 35-45 parts of herba cynomorii, 30-35 parts of yucca, 25-35 parts of watermelon peel, 20-30 parts of banana peel, 15-25 parts of rhizoma belamcandae, 15-23 parts of pitaya peel, 10-15 parts of cortex acanthopanacis semticosi and 5-10 parts of folium mori. The invention further discloses a preparation method of the chicken anti-stress immunopotentiator in the high-temperature and high-humidity state and application of the chicken anti-stress immunopotentiator in the high-temperature and high-humidity state to prevention and treatment of chicken stress reaction in the high-temperature and high-humidity environment. The chicken anti-stress immunopotentiator in the high-temperature and high-humidity state can quickly act on intestinal tracts of chickens to relieve discomfort caused by the high-temperature and high-humidity state, so that the anti-stress effect is achieved, and feed mildew can be prevented by adding the chicken anti-stress immunopotentiator into feed; and pathogenic microorganisms in water can be effectively restrained by adding the compound into drinking water.

The present application belongs to the technical field of biological medicine, and relates to a kind of Parachlorella sp. Polysaccharide and its preparation method and application. The present application obtains a kind of uniform Parachlorella sp. Polysaccharide NGP-1 by water extraction, alcohol precipitation, column chromatography separation to Parachlorella sp.;Combining high performance liquid chromatography, methylation combined gas chromatography-mass spectrometry, nuclear magnetic resonance and other methods to determine its structural characteristics. The obtained NGP-1 can promote macrophage proliferation, enhance phagocytic ability, improve active oxygen level, induce nitric oxide and cytokine production, and up-regulate the expression of corresponding mRNA, and has significant immune enhancing activity. The Parachlorella sp. Polysaccharide NGP-1 prepared by the present application can be used as a natural immune enhancer, which widens the application range of Parachlorella sp. And has broad application prospect.

The invention discloses a lycium barbarum polysaccharide-ganoderma triterpene supramolecule and a preparation method and application thereof, and the preparation method comprises the following steps: dissolving LBP powder in water to obtain an LBP solution; dissolving the Glts powder in an organic solvent to obtain a Glts solution; stirring the LBP solution at a constant speed, slowly dropwise adding the Glts solution into the LBP solution by using a pipette, and continuously stirring and carrying out ultrasonic treatment to obtain an LG supramolecular solution; the novel immunopotentiator is constructed by combining the advantages of the lycium barbarum polysaccharide and the ganoderma triterpenes for the first time, and the advantage of interdisciplinary is embodied. Compared with pure ganoderma triterpenes, the LG supramolecules can solve the problem of solubility of Glts, and the LG supramolecules and the lycium barbarum polysaccharide jointly play a better immune role.

The application discloses application of KLF12 or a KLF12 expression promoter in preparation of a medicine for inhibiting Galectin-1 expression, and can inhibit Galectin-1 expression, improve CD8 + T cell infiltration, enhance CD8 + T cell function, and obviously enhance the in-vivo treatment effect of anti-PD-1 on tumor patients, so that the KLF12 or the KLF12 expression promoter can be used for preparing the medicine for inhibiting Galectin-1 expression, and can be used for improving CD8 + T cell infiltration, enhancing CD8 + T cell immune function, and is used as an immune enhancer; the combination of anti-PD-1 and (i) the KLF12 or the KLF12 expression promoter or (ii) the medicine for inhibiting Galectin-1 expression is prepared into an anti-tumor medicine, which has a good application prospect.

The present invention relates to a composition for enhancing immunity, which contains a mixture of a mixed extract of angelica, cnidium, and paeonia and a polysaccharide isolated therefrom; or a fraction thereof as an effective ingredient. The composition containing a mixture of a mixed extract of angelica, cnidium, and paeonia and a polysaccharide isolated therefrom; or a fraction thereof as an effective ingredient according to the present invention increases body weight, increases WBC count, restores spleen cell density, increases spleen cell proliferation ability, and increases immune-related cytokines as well as significantly increases NK cell activity, particularly with respect to innate immunity, and can be thus applied as an immune-enhancing agent or a food composition for enhancing immunity.

The present application relates to the field of macrobrachium rosenbergii feed, and particularly relates to a macrobrachium rosenbergii feed resisting decapod iridovirus disease and a production process thereof, the feed is composed of base feed and 1.0%-2.0% three-year-old gahnia filiformis super-fine powder, the production process includes steps of three-year-old gahnia filiformis airing and crushing, base raw material pretreatment and mixing, low-temperature granulation, 65 DEG C drying and cooling, vacuum packaging and storage, and the whole process guarantees the activity of gahnia filiformis effective components; under the premise of not affecting the growth performance of macrobrachium rosenbergii, the present application significantly improves the resistance of macrobrachium rosenbergii to decapod iridovirus disease, improves intestinal tissue structure, maintains intestinal flora stability, and improves serum non-specific immune enzyme activity; meanwhile, the present application solves the problems of poor stability, insufficient targeting, and low activity retention rate in processing of the existing immune enhancer, realizes the synergistic effect of feed nutrition and antiviral immunity, and provides an effective scheme for macrobrachium rosenbergii healthy breeding.

This invention relates to a fish interferon regulator IRF4 gene, protein, expression vector, and their applications, belonging to the field of fish molecular immunology technology. The fish interferon regulator IRF4 is derived from the oval pomfret, and the cDNA nucleotide sequence of the oval pomfret IRF4 gene is shown in SEQ ID NO.1. The amino acid sequence of the protein encoded by the oval pomfret IRF4 gene is shown in SEQ ID NO.2. This invention also provides an expression vector containing the oval pomfret IRF4 gene, and an anti-Vibrio harveyi infection preparation containing the protein or a eukaryotic expression vector. The oval pomfret IRF4 protein of this invention can promote the respiratory burst activity and phagocytic capacity of oval pomfret head kidney macrophages. Overexpression in oval pomfret... Believe After IRF4 is activated, the body's antibacterial ability can be significantly improved, and it can be used as an immune enhancer.