17 results about "IMMUNE ENHANCEMENT AGENTS" patented technology

The invention relates to the technical field of aquatic animal immune regulation and control, in particular to rHoLL-like stepped lectin-like recombinant protein as well as a preparation method and application thereof. The recombinant protein has pathogen-related molecular pattern recognition and combination capabilities, can promote aggregation of pathogens in a body fluid environment, and activates an aquatic animal complement system, so that the non-specific immune defense capability is enhanced. The preparation method comprises the following steps: amplifying a HoLL-like mature peptide coding sequence, constructing a recombinant expression vector, and converting the recombinant expression vector into an escherichia coli expression host for induced expression to obtain rHoLL-like lectin-like recombinant protein existing in an inclusion body form; and carrying out affinity chromatography purification on the inclusion body protein, and carrying out renaturation treatment in a manner of gradient reduction of denaturant concentration to obtain the rHoLL-like stepped lectin-like recombinant protein. The rHoLL-like stepped lectin-like recombinant protein has good biological safety, and can be applied to aquaculture as an aquatic animal immunopotentiator or a related biological product.

The application provides a turbot compound immune enhancer, a preparation method and application thereof. The application discloses application of a composition containing a series of components in preparation of a turbot immune enhancer, and the immune enhancer can effectively improve expression of a proinflammatory related gene of the turbot, reduce a number of bacteria in a liver or an intestinal tract, enhance a bactericidal capacity of the turbot, enhance an antioxidant stress capacity of the turbot, relieve liver damage of the turbot after being infected by bacteria, and relieve tissue damage of the turbot after being infected by bacteria. The immune enhancer component is simple, environment-friendly, low in cost and ideal in effect, and provides a new scheme for improving a breeding level of marine fish and improving a survival rate.

This invention discloses a method, apparatus, equipment, and storage medium for the synergistic blocking of harmful substances in chicken houses. The method includes collecting environmental parameters, physiological states of the flock, and pathogen information from the chicken house; sending the collected information to an intelligent decision-making system; and when parameters exceed thresholds or pathogenic agents are detected, the intelligent decision-making system generates a synergistic control strategy and triggers a coordinated response: a light disinfection system activates a specific wavelength light source to form an effective disinfection area; a ventilation system guides airflow into the effective disinfection area; a drinking water disinfection system adds effective disinfectants and immune enhancers to the drinking water; and the intelligent decision-making system dynamically adjusts the parameters. In this embodiment, the light disinfection system, ventilation system, and drinking water disinfection system not only achieve the effect of a single disinfection method, but also work together to significantly reduce the risk of highly contagious diseases in the flock, ensuring the normal growth of the chickens and avoiding economic losses caused by diseases.

The invention relates to a Gleditsia sinensis seed ethanol extract and a preparation method and application thereof in aquatic animal immunization, the preparation method of the Gleditsia sinensis seed ethanol extract comprises the following steps: crushing Gleditsia sinensis seeds, adding 75-80% ethanol solution according to a solid-to-liquid ratio of 1: 8-1: 10, and extracting; during extraction, heating is performed to 80-85 DEG C, reflux extraction is performed 2-3 times, and each time lasts for 1.5-2 hours; combining all extracting solutions, and filtering under reduced pressure to obtain clear filtrate; and concentrating the filtrate to 1 / 5 of the original volume under reduced pressure by using a rotary evaporator, and freeze-drying the concentrated solution to obtain faint yellow powder. The gleditsia sinensis seed ethanol extract is applied to improvement of the immune function of aquatic animals. The invention provides a novel immunopotentiator, namely the gleditsia sinensis seed extract, for aquaculture. The immunopotentiator is low in cost, simple in preparation process, remarkable in immunopotentiation effect, safe, environment-friendly and suitable for being mixed with feed for feeding aquatic animals.

This invention relates to the field of extracts, specifically to a method for preparing a traditional Chinese medicine extract and its application. The method includes pulverizing and sieving a traditional Chinese medicine formula, preparing a compound enzymatic hydrolysate using a compound enzyme, mixing the powdered medicine with the hydrolysate, performing enzymatic hydrolysis, ultrasonic extraction, fermentation, filtration, and freeze-drying to obtain the traditional Chinese medicine extract. The extract is used as an immune enhancer for animals. Existing methods for preparing traditional Chinese medicine extracts suffer from low extraction efficiency and poor drug efficacy. The method for preparing a traditional Chinese medicine extract and its application provided by this invention offers higher extraction efficiency and better drug efficacy.

The invention relates to a compound atomized liquid for pet respiratory mucosa and a preparation method thereof. Each 100 mL of the compound atomized liquid is prepared from 0.15 to 0.20 g of a liquorice root extract, 0.18 to 0.25 g of L-lysine hydrochloride, 0.20 to 0.25 g of chitosan oligosaccharide, 0.25 to 0.35 g of yeast-beta-glucan, 0.008 to 0.015 g of EDTA-2Na (Ethylene Diamine Tetraacetic Acid), 0.4 to 0.6 g of trehalose, 0.65 to 0.75 g of sodium chloride, 0.5 to 1.0 mg of micromolecular antimicrobial peptide and the balance of citric acid / phosphate buffer solution. Wherein the molecular weight of the chitosan oligosaccharide is less than or equal to 2000Da; the pH value of the compound atomized liquid is 6.0-7.0; and the osmotic pressure of the compound atomized liquid is 280-320 mOsm / kg. By compounding the natural anti-inflammatory component, the immunopotentiator, the mucosa protective agent and the micromolecular antimicrobial peptide, synergistic treatment is realized, and side effects are reduced.

The present disclosure is generally directed to an immunogenic protein conjugate comprising anthrax toxin protective antigen (PA) protein as an immune enhancer, a peptide linker, and an antigen derived from an infectious or pathogenic virus or microorganism. This protein conjugate can be used to induce an immune response in a subject, such as in a vaccine against the antigen of the protein conjugate. For example, the antigen can be Spy0469 for a vaccine against S. pyogenes.

The invention belongs to the technical field of aquaculture, and discloses an immunopotentiator for aquaculture and application of the immunopotentiator. The immunopotentiator for aquaculture is prepared from the following components in parts by weight: 50 to 60 parts of ginsenoside, 15 to 20 parts of organic saccharicterpenin, 6 to 10 parts of a nutritional supplement, 1 to 5 parts of an immunopotentiating aid, 0.5 to 2 parts of an anti-stress aid and 0.2 to 1 part of an antioxidant. The ginsenoside and the organic saccharicterpenin which are naturally extracted are used as core active ingredients, safe auxiliaries are used as auxiliary materials, harmful ingredients such as antibiotics and hormones do not exist, medicine residues cannot be generated after the composition is used, and safety is high; meanwhile, through scientific compounding, all the components play a synergistic role, the non-specific immunity and anti-stress ability of aquatic animals can be improved, intestinal microecological balance can be improved, growth can be promoted, the culture water environment can be indirectly improved, and multiple effects are achieved.

The present application belongs to the technical field of biological medicine, and relates to a kind of Parachlorella sp. Polysaccharide and its preparation method and application. The present application obtains a kind of uniform Parachlorella sp. Polysaccharide NGP-1 by water extraction, alcohol precipitation, column chromatography separation to Parachlorella sp.;Combining high performance liquid chromatography, methylation combined gas chromatography-mass spectrometry, nuclear magnetic resonance and other methods to determine its structural characteristics. The obtained NGP-1 can promote macrophage proliferation, enhance phagocytic ability, improve active oxygen level, induce nitric oxide and cytokine production, and up-regulate the expression of corresponding mRNA, and has significant immune enhancing activity. The Parachlorella sp. Polysaccharide NGP-1 prepared by the present application can be used as a natural immune enhancer, which widens the application range of Parachlorella sp. And has broad application prospect.

The application discloses application of KLF12 or a KLF12 expression promoter in preparation of a medicine for inhibiting Galectin-1 expression, and can inhibit Galectin-1 expression, improve CD8 + T cell infiltration, enhance CD8 + T cell function, and obviously enhance the in-vivo treatment effect of anti-PD-1 on tumor patients, so that the KLF12 or the KLF12 expression promoter can be used for preparing the medicine for inhibiting Galectin-1 expression, and can be used for improving CD8 + T cell infiltration, enhancing CD8 + T cell immune function, and is used as an immune enhancer; the combination of anti-PD-1 and (i) the KLF12 or the KLF12 expression promoter or (ii) the medicine for inhibiting Galectin-1 expression is prepared into an anti-tumor medicine, which has a good application prospect.

The present application relates to the field of macrobrachium rosenbergii feed, and particularly relates to a macrobrachium rosenbergii feed resisting decapod iridovirus disease and a production process thereof, the feed is composed of base feed and 1.0%-2.0% three-year-old gahnia filiformis super-fine powder, the production process includes steps of three-year-old gahnia filiformis airing and crushing, base raw material pretreatment and mixing, low-temperature granulation, 65 DEG C drying and cooling, vacuum packaging and storage, and the whole process guarantees the activity of gahnia filiformis effective components; under the premise of not affecting the growth performance of macrobrachium rosenbergii, the present application significantly improves the resistance of macrobrachium rosenbergii to decapod iridovirus disease, improves intestinal tissue structure, maintains intestinal flora stability, and improves serum non-specific immune enzyme activity; meanwhile, the present application solves the problems of poor stability, insufficient targeting, and low activity retention rate in processing of the existing immune enhancer, realizes the synergistic effect of feed nutrition and antiviral immunity, and provides an effective scheme for macrobrachium rosenbergii healthy breeding.

This invention relates to a fish interferon regulator IRF4 gene, protein, expression vector, and their applications, belonging to the field of fish molecular immunology technology. The fish interferon regulator IRF4 is derived from the oval pomfret, and the cDNA nucleotide sequence of the oval pomfret IRF4 gene is shown in SEQ ID NO.1. The amino acid sequence of the protein encoded by the oval pomfret IRF4 gene is shown in SEQ ID NO.2. This invention also provides an expression vector containing the oval pomfret IRF4 gene, and an anti-Vibrio harveyi infection preparation containing the protein or a eukaryotic expression vector. The oval pomfret IRF4 protein of this invention can promote the respiratory burst activity and phagocytic capacity of oval pomfret head kidney macrophages. Overexpression in oval pomfret... Believe After IRF4 is activated, the body's antibacterial ability can be significantly improved, and it can be used as an immune enhancer.

The present application relates to the field of feed additives, and discloses a use of N-methyl-D-aspartate for improving growth performance of fish, improving muscle quality and enhancing ammonia nitrogen stress resistance. The present application aims at solving the problems of growth performance reduction, fish muscle quality reduction and vulnerability to water body ammonia nitrogen stress in current fish culture, and provides a use of N-methyl-D-aspartate for improving growth performance of fish, improving muscle quality and enhancing ammonia nitrogen stress resistance, i.e. using NMDA as a feed additive, growth promoter, meat quality improver or immune enhancer to improve fish meat quality, improve fish meat flavor, significantly improve the growth performance of Xiangyun crucian, improve the muscle quality and flavor, enhance the ammonia nitrogen stress resistance, and significantly increase the economic benefits of fish farmers.

The present application relates to a recombinant antigen protein for preventing acute hepatopancreatic necrosis disease in shrimp and a vaccine composition comprising same. The recombinant antigen protein according to one aspect of the present invention can maintain stability under seawater treatment conditions, does not affect the growth of shrimp, and can significantly lower the mortality rate of shrimp, and thus can be effectively used as an immune enhancer or a feed additive in shrimp farming.

The present application provides an immune enhancer for largemouth bass, comprising astragalus polysaccharide and nucleotides with improved immune effect; further, the immune enhancer further comprises forsythia suspensa polysaccharide and / or largeleaf figleaf ginseng polysaccharide; preferably, the immune enhancer further comprises vitamin C; further, the immune enhancer further comprises protein peptide. The immune enhancer for largemouth bass provided by the present application has the effect of resisting largemouth bass rhabdovirus MSRV, can inhibit the replication of largemouth bass rhabdovirus MSRV; and the protein peptide used has antibacterial activity, thereby effectively controlling the reproduction of pathogenic bacteria of largemouth bass.

This invention belongs to the field of acidifier technology, specifically relating to an acidifier that can improve the health of breeding ducks and the hatchability of hatching eggs, its preparation method, and its application. The acidifier includes acidifier A and acidifier B. Acidifier A is prepared by mixing and adjusting the volume of calcium formate, acetic acid, lactic acid, monk fruit extract, and an immune enhancer; acidifier B is prepared by mixing and adjusting the volume of calcium propionate, citric acid, malic acid, stevia extract, and an antioxidant. This invention enhances the immunity and antioxidant capacity of breeding ducks and improves eggshell quality by intermittently and alternately adding the two acidifiers to their drinking water during the egg-laying period. Furthermore, by spraying a proportionally diluted mixture of the two acidifiers during the later stages of hatching, it improves embryonic calcium absorption and duckling shell-pecking rate, reduces embryonic mortality, and significantly increases the hatchability of hatching eggs. This invention is suitable for large-scale breeding and hatching of breeding ducks.

This invention belongs to the field of food fermentation and functional beverage technology, specifically relating to a strain of *Lactobacillus fermentans* and its application in the preparation of fermented goji berry juice that enhances immunity. The *Lactobacillus fermentans* strain LFY21 was deposited on November 17, 2025, at the China Center for Type Culture Collection (CCTCC), with accession number CCTCC NO: M 20252569; the deposit address is Wuhan University. This strain grows vigorously in goji berry juice, but exhibits weak acid production during fermentation, resulting in a high sugar-acid ratio, good flavor and palatability, and high free radical scavenging activity and total phenol content. Furthermore, animal experiments have demonstrated that fermented goji berry juice prepared with this strain can effectively enhance immune function, showing potential as an immune enhancer.