Composite probiotic powder capable of nourishing stomach and tonifying spleen and preparation method of composite probiotic powder

Abstract

The invention provides composite probiotic powder capable of nourishing the stomach and tonifying the spleen and a preparation method of the composite probiotic powder. The composite probiotic powder comprises the following components in parts by weight: 6-12 parts of freeze-dried bacterial powder, 40-50 parts of prebiotics, 20-25 parts of fruit and vegetable powder, 14-19 parts of enzymolysis plant powder, 12-15 parts of fermented plant powder and 46-61 parts of sugar alcohol. The freeze-dried bacterial powder is prepared by mixing lactobacillus acidophilus, bifidobacterium animalis, lactobacillus plantarum, lactobacillus helveticus and lactobacillus bulgaricus; the prebiotics are prepared by mixing inulin, galactooligosaccharide, isomaltooligosaccharide and fructo-oligosaccharide in a weight ratio of 10: (8-12): (4-6): (13-16). The prepared compound probiotic powder has the effects of nourishing the stomach and tonifying the spleen.

Description

Technical Field

[0001] The invention belongs to the technical field of health care products, and particularly relates to a stomach-nourishing and spleen-strengthening composite probiotic powder and a preparation method thereof. Background Art

[0002] The spleen and stomach refer to the spleen meridian and stomach meridian. When the spleen and stomach are weak, if people do not control their diet and often eat raw, cold and cool food, it will cause cold and dampness to damage the spleen, and the digestion, absorption and transportation functions of the food eaten will be reduced, showing symptoms such as abdominal distension, pain, noise, belching, heartburn, and loss of appetite. At present, there are many Western medicines for the treatment of gastric ulcers, which can only eliminate the surface pain symptoms, but not the root cause of the disease. In addition, long-term use has great side effects on the human body, and it is easy to relapse after treatment, and it is often difficult to cure.

[0003] Probiotic powders for nourishing the stomach and spleen are products designed to support digestive health by supplementing beneficial bacteria. Such products usually contain probiotic strains that can help maintain the balance of intestinal microecology, as well as some auxiliary ingredients, such as prebiotics, to promote gastrointestinal health. The probiotic powders for nourishing the stomach and spleen currently on the market have certain effects, but the recurrence rate after recovery is high, and the long-term effect is not ideal.

[0004] Therefore, there is an urgent need for a composite probiotic powder for nourishing the stomach and spleen and a preparation method thereof. Summary of the invention

[0005] The purpose of the present invention is to provide a stomach-nourishing and spleen-strengthening composite probiotic powder and a preparation method thereof.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] A stomach-nourishing and spleen-strengthening composite probiotic powder comprises the following components in parts by weight: 6-12 parts of freeze-dried bacterial powder, 40-50 parts of prebiotics, 20-25 parts of fruit and vegetable powder, 14-19 parts of enzymatically hydrolyzed plant powder, 12-15 parts of fermented plant powder and 46-61 parts of sugar alcohol; the freeze-dried bacterial powder is a mixture of Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus plantarum, Lactobacillus helveticus and Lactobacillus bulgaricus; the prebiotics are a mixture of inulin, galacto-oligosaccharide, isomaltooligosaccharide and fructo-oligosaccharide in a weight ratio of 10:(8-12):(4-6):(13-16).

[0008] Furthermore, the sugar alcohol is a mixture of lactitol, erythritol and xylitol in a weight ratio of 1:(1.3-1.5):(0.4-0.7).

[0009] Furthermore, the fruit and vegetable powder comprises the following raw materials in parts by weight: 2-6 parts of apple powder, 11-14 parts of banana powder, 4-10 parts of papaya powder, 5-8 parts of pitaya powder, and 6-12 parts of pumpkin powder.

[0010] Furthermore, the number of live Lactobacillus acidophilus in the freeze-dried bacterial powder was 3.0×10 9 CFU / g-6.0×10 9 CFU / g, number of live Bifidobacterium animalis 5.0×10 8 CFU / g-8.0×10 8 CFU / g, number of live Lactobacillus plantarum 0.8×10 9 CFU / g-2.0×10 9 CFU / g, the number of live Lactobacillus helveticus is 8.0×10 9 CFU / g-11.0×10 9 CFU / g, number of live Lactobacillus bulgaricus 1.0×10 9 CFU / g-3.0×10 9 CFU / g.

[0011] The preparation method of freeze-dried bacterial powder is as follows: the strains of Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus plantarum, Lactobacillus helveticus and Lactobacillus bulgaricus are respectively expanded and cultured for three times, and then cultured in conventional sterilized culture media respectively, and then mixed to obtain bacterial liquid; the bacterial liquid is centrifuged and washed with physiological saline to obtain a high-concentration bacterial liquid; the high-concentration bacterial liquid is protected by a freeze-drying protective agent to protect the bacterial activity to obtain freeze-dried powder.

[0012] The composite probiotic powder on the market currently usually selects several strains of Lactobacillus acidophilus, Bifidobacterium lactis and Lactobacillus rhamnosus for compounding, which has a certain therapeutic effect for spleen and stomach weakness. But the cure rate is not ideal, and the number of relapses in the later period is relatively large. The present invention adds specific concentrations of Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus plantarum, Lactobacillus helveticus and Lactobacillus bulgaricus to the composite probiotic powder, which improves the cure rate for treating spleen and stomach weakness symptoms. These strains act synergistically, increase the ratio of beneficial bacteria in the intestinal tract, reduce the number of harmful bacteria, thereby improve intestinal microecology, help decompose food, promote the absorption of nutrients, and enhance the immune function of the body by improving intestinal health. But the reduction of the relapse rate after recovery is not ideal.

[0013] The freeze-drying protective agent includes, by mass concentration, 130 g / L of trehalose, 50 g / L of maltodextrin, 35 g / L of glycerol, and the balance of water.

[0014] Furthermore, the preparation method of the enzymatic hydrolyzed plant powder is: fresh strawberries, mulberries and raspberries are mixed, the surface water is drained, crushed, water is added to obtain a mixed solution A, cellulase is added, enzymolysis is carried out at 40-45°C for 4-6 hours, and the enzyme is inactivated; pectinase is continued to be added, enzymolysis is carried out at 40-45°C for 3-5 hours, the enzyme is inactivated, filtered, dried, crushed, and sieved to obtain the enzymatic hydrolyzed plant powder.

[0015] Furthermore, the weight ratio of strawberry, mulberry and raspberry is 1:(1.4-1.7):(0.2-0.5).

[0016] Furthermore, the enzymatic activity of cellulase in the mixed solution A is 80U / mL-100U / mL; the enzymatic activity of pectinase in the mixed solution A is 140U / mL-160U / mL.

[0017] Furthermore, the preparation method of the fermented plant powder is: fresh garcinia cambogia, blueberry and cherry are mixed, the surface water is drained, crushed, water is added to obtain a mixed solution B, Bifidobacterium breve is added, fermented at 40-45°C for 1-3 days, and sterilized; Lactobacillus casei is continued to be added, fermented at 40-45°C for 3-6 days, sterilized, filtered, dried, crushed, and sieved to obtain the fermented plant powder.

[0018] Furthermore, the weight ratio of garcinia cambogia, blueberry and cherry is (1.2-1.4):(0.6-0.8):1.

[0019] Furthermore, the bacterial activity of Bifidobacterium breve in the mixed solution B was 10 10 CFU / mL-10 11 CFU / mL; the bacterial activity of Lactobacillus casei in mixed solution B is 10 8 CFU / mL-10 9 CFU / mL.

[0020] In order to improve the recurrence rate of spleen and stomach discomfort after recovery of composite probiotic powder, the present invention attempts to add the enzymatic hydrolysis products of strawberry, mulberry and raspberry. After testing, it was found that there was a certain improvement effect after adding, but the ideal goal was still not reached. Continue to add fermented plant powder prepared by garcinia cambogia, blueberry and cherry in the present invention. Surprisingly, the system of the present invention can well improve the recurrence rate of spleen and stomach discomfort after recovery of composite probiotic powder by adding enzymatic hydrolysis plant powder and fermented plant powder at the same time. By adding enzymatic hydrolysis plant powder and fermented plant powder in the composite probiotic system at the same time, metabolites such as organic acids and short-chain fatty acids produced in the enzymatic hydrolysis and fermentation process can regulate the intestinal pH value, inhibit the growth of harmful bacteria, and promote the proliferation of beneficial bacteria. Plant components are decomposed into smaller molecules during enzymatic hydrolysis and fermentation, which are more easily absorbed and utilized by the human body. The prebiotics produced during enzymatic hydrolysis and fermentation can be used as food for probiotics to promote the growth of probiotics, thereby improving the intestinal microecological balance. Adding enzymatic plant powder and fermented plant powder to the system can not only improve the activity and stability of probiotics, but also fundamentally improve the spleen and stomach function by improving digestion and absorption, regulating the intestinal environment, etc., thereby reducing the recurrence rate of spleen and stomach discomfort after recovery.

[0021] Furthermore, the weight ratio of freeze-dried bacterial powder, prebiotics and sugar alcohol is 10:(43-46):(51-54).

[0022] The storage stability of composite probiotic powder is also a major problem that troubles manufacturers. When the probiotic powder deteriorates, the following phenomena may occur: Color change: Normal probiotic powder is usually white or light yellow. If the color becomes abnormal or spots appear. Odor change: Fresh probiotic powder usually has no obvious odor or has a slight fermentation odor. If a strong sour, musty or other unusual odor appears, it indicates that the probiotic powder may have deteriorated. Agglomeration or coagulation: Dry probiotic powder should be in a loose powder state. The storage stability is low, and caking or coagulation is prone to occur. The freeze-dried bacterial powder, prebiotics and sugar alcohol of the present invention are compounded in a specific ratio, and the obtained composite probiotic powder has high storage stability.

[0023] The present invention also provides a method for preparing a composite probiotic powder for nourishing the stomach and strengthening the spleen, comprising the following steps: mixing prebiotics, fruit and vegetable powder, enzymatically hydrolyzed plant powder, fermented plant powder and sugar alcohol, spraying alcohol to make them bond and granulate, and then mixing the mixture with freeze-dried bacterial powder to obtain the composite probiotic powder.

[0024] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0025] 1. The present invention adds specific concentrations of Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus plantarum, Lactobacillus helveticus and Lactobacillus bulgaricus to the composite probiotic powder, thereby improving the cure rate of treating spleen and stomach deficiency symptoms.

[0026] 2. The system of the present invention can significantly improve the recurrence rate of spleen and stomach discomfort after recovery caused by the composite probiotic powder by adding enzymolysis plant powder and fermentation plant powder at the same time.

[0027] 3. The freeze-dried bacterial powder, prebiotics and sugar alcohol of the present invention are compounded in a specific ratio, and the prepared composite probiotic powder has high storage stability. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] The bacteria used in the present invention are commercially available products, specifically as follows:

[0030] Lactobacillus acidophilus, accession number: CCTCCAB2010208, was purchased from China Center for Type Culture Collection.

[0031] Bifidobacterium animalis, accession number: SHBCCD24416AS1.2891, was purchased from Shanghai Microbiological Collection Center.

[0032] Lactobacillus plantarum, accession number: CCTCCAB2010210, was purchased from China Center for Type Culture Collection.

[0033] Lactobacillus helveticus, accession number: CCTCCAB2010205, was purchased from China Center for Type Culture Collection.

[0034] Lactobacillus bulgaricus, accession number: CCTCCCB20082295, was purchased from China Center for Type Culture Collection.

[0035] Bifidobacterium breve, accession number: SHBCCD24424JCM7017, was purchased from Shanghai Microbiological Collection Center.

[0036] Lactobacillus casei, accession number: SHBCCD24737, was purchased from Shanghai Microbiological Collection Center.

[0037] Lactobacillus brevis, accession number: SHBCCD14346, was purchased from Shanghai Microbiological Collection Center.

[0038] Bifidobacterium longum, accession number: SHBCCD24310, was purchased from Shanghai Microbiological Collection Center.

[0039] Bifidobacterium lactis, accession number: SHBCCD24728, was purchased from Shanghai Microbiological Collection Center.

[0040] Lactobacillus rhamnosus, accession number: SHBCCD73158, was purchased from Shanghai Microbiological Collection Center.

[0041] Example 1

[0042] The present embodiment provides a composite probiotic powder for nourishing the stomach and strengthening the spleen, comprising the following components in parts by weight: 10 parts of freeze-dried bacterial powder, 45 parts of prebiotics, 22 parts of fruit and vegetable powder, 16 parts of enzymatic plant powder, 13 parts of fermented plant powder and 53 parts of sugar alcohol;

[0043] The freeze-dried bacterial powder is a mixture of Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus plantarum, Lactobacillus helveticus and Lactobacillus bulgaricus;

[0044] The prebiotics are a mixture of inulin, galacto-oligosaccharide, isomalto-oligosaccharide and fructo-oligosaccharide in a weight ratio of 10:9:5:15.

[0045] The sugar alcohol is a mixture of lactitol, erythritol and xylitol in a weight ratio of 1:1.4:0.6.

[0046] The fruit and vegetable powder comprises the following raw materials in parts by weight: 5 parts of apple powder, 12 parts of banana powder, 6 parts of papaya powder, 7 parts of dragon fruit powder and 10 parts of pumpkin powder.

[0047] The preparation method of freeze-dried bacterial powder is as follows: the strains of Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus plantarum, Lactobacillus helveticus and Lactobacillus bulgaricus are respectively expanded and cultured for three times, and then cultured in conventional sterilized culture media respectively, and then mixed to obtain bacterial liquid; the bacterial liquid is centrifuged and washed with physiological saline to obtain a high-concentration bacterial liquid; the high-concentration bacterial liquid is protected by a freeze-drying protective agent to protect the bacterial activity to obtain freeze-dried powder.

[0048] The number of live Lactobacillus acidophilus in the freeze-dried bacterial powder is 5×10 9 CFU / g, the number of live Bifidobacterium animalis 6×10 8 CFU / g, number of live Lactobacillus plantarum 1×10 9 CFU / g, the number of live Lactobacillus helveticus is 10×10 9 CFU / g and the number of live Lactobacillus bulgaricus is 2×10 9 CFU / g.

[0049] The freeze-drying protective agent comprises, by mass concentration, 130 g / L of trehalose, 50 g / L of maltodextrin and 35 g / L of glycerol, and the balance is water.

[0050] The preparation method of the enzymatic hydrolysis plant powder comprises the following steps: mixing fresh strawberries, mulberries and raspberries in a weight ratio of 1:1.5:0.4, draining surface water, crushing, adding water to obtain a mixed solution A, adding cellulase, performing enzymatic hydrolysis at 42°C for 5 hours, and inactivating the enzyme; continuously adding pectinase, performing enzymatic hydrolysis at 42°C for 4 hours, inactivating the enzyme, filtering, drying, crushing and sieving to obtain the enzymatic hydrolysis plant powder.

[0051] The enzymatic activity of cellulase in mixed solution A is 90 U / mL; the enzymatic activity of pectinase in mixed solution A is 150 U / mL.

[0052] The preparation method of the fermented plant powder comprises the following steps: mixing fresh garcinia cambogia, blueberry and cherry in a weight ratio of 1.3:0.7:1, draining surface water, crushing, adding water to obtain a mixed solution B, adding Bifidobacterium breve, fermenting at 42°C for 2 days, and sterilizing; continuously adding Lactobacillus casei, fermenting at 42°C for 4 days, sterilizing, filtering, drying, crushing and sieving to obtain the fermented plant powder.

[0053] The bacterial activity of Bifidobacterium breve in mixed solution B is 10 10 CFU / mL; the bacterial activity of Lactobacillus casei in mixed solution B is 10 9 CFU / mL.

[0054] The preparation method of the composite probiotic powder for nourishing the stomach and strengthening the spleen comprises the following steps: mixing prebiotics, fruit and vegetable powder, enzymatically hydrolyzed plant powder, fermented plant powder and sugar alcohol, spraying alcohol to make them bonded and granulated, and then mixing the mixture with freeze-dried bacterial powder to obtain the composite probiotic powder.

[0055] Example 2

[0056] The present embodiment provides a composite probiotic powder for nourishing the stomach and strengthening the spleen, comprising the following components in parts by weight: 10 parts of freeze-dried bacterial powder, 43 parts of prebiotics, 20 parts of fruit and vegetable powder, 19 parts of enzymatic plant powder, 12 parts of fermented plant powder and 54 parts of sugar alcohol; the freeze-dried bacterial powder is a mixture of Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus plantarum, Lactobacillus helveticus and Lactobacillus bulgaricus; the prebiotics are a mixture of inulin, galacto-oligosaccharides, isomaltooligosaccharides and fructo-oligosaccharides in a weight ratio of 10:8:6:13.

[0057] The sugar alcohol is a mixture of lactitol, erythritol and xylitol in a weight ratio of 1:1.3:0.7.

[0058] The fruit and vegetable powder comprises the following raw materials in parts by weight: 2 parts of apple powder, 14 parts of banana powder, 4 parts of papaya powder, 8 parts of dragon fruit powder, and 12 parts of pumpkin powder.

[0059] The preparation method of freeze-dried bacterial powder is as follows: the strains of Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus plantarum, Lactobacillus helveticus and Lactobacillus bulgaricus are respectively expanded and cultured for three times, and then cultured in conventional sterilized culture media respectively, and then mixed to obtain bacterial liquid; the bacterial liquid is centrifuged and washed with physiological saline to obtain a high-concentration bacterial liquid; the high-concentration bacterial liquid is protected by a freeze-drying protective agent to protect the bacterial activity to obtain freeze-dried powder.

[0060] The number of live Lactobacillus acidophilus in the freeze-dried bacterial powder is 3.0×10 9 CFU / gCFU / g, the number of live bacteria of animal Bifidobacterium is 8.0×10 8 CFU / g, number of live Lactobacillus plantarum 0.8×10 9 CFU / g, the number of live Lactobacillus helveticus is 11.0×10 9 CFU / g and the number of live Lactobacillus bulgaricus is 1.0×10 9 CFU / g.

[0061] The freeze-drying protective agent includes, by mass concentration, 130 g / L of trehalose, 50 g / L of maltodextrin and 35 g / L of glycerol.

[0062] The preparation method of the enzymatic hydrolysis plant powder comprises the following steps: mixing fresh strawberries, mulberries and raspberries in a weight ratio of 1:1.4:0.2, draining surface water, crushing, adding water to obtain a mixed solution A, adding cellulase, performing enzymatic hydrolysis at 40°C for 6 hours, and inactivating the enzyme; continuously adding pectinase, performing enzymatic hydrolysis at 40°C for 5 hours, inactivating the enzyme, filtering, drying, crushing and sieving to obtain the enzymatic hydrolysis plant powder.

[0063] The enzymatic activity of cellulase in the mixed solution A is 80 U / mL; the enzymatic activity of pectinase in the mixed solution A is 160 U / mL.

[0064] The preparation method of the fermented plant powder comprises the following steps: mixing fresh garcinia cambogia, blueberry and cherry in a weight ratio of 1.2:0.8:1, draining surface water, crushing, adding water to obtain a mixed solution B, adding Bifidobacterium breve, fermenting at 40°C for 3 days, and sterilizing; continuously adding Lactobacillus casei, fermenting at 40°C for 6 days, sterilizing, filtering, drying, crushing and sieving to obtain the fermented plant powder.

[0065] The bacterial activity of Bifidobacterium breve in mixed solution B is 10 10 CFU / mL; the bacterial activity of Lactobacillus casei in mixed solution B is 10 9 CFU / mL.

[0066] The preparation method of the composite probiotic powder for nourishing the stomach and strengthening the spleen comprises the following steps: mixing prebiotics, fruit and vegetable powder, enzymatically hydrolyzed plant powder, fermented plant powder and sugar alcohol, spraying alcohol to make them bonded and granulated, and then mixing the mixture with freeze-dried bacterial powder to obtain the composite probiotic powder.

[0067] Comparative Example 1

[0068] The difference between this comparative example and Example 1 is: a composite probiotic powder for nourishing the stomach and strengthening the spleen, comprising the following components in parts by weight: 15 parts of freeze-dried bacterial powder, 37 parts of prebiotics, 17 parts of fruit and vegetable powder, 22 parts of enzymatic plant powder, 7 parts of fermented plant powder and 61 parts of sugar alcohol.

[0069] Comparative Example 2

[0070] The difference between this comparative example and Example 1 is that no fermented plant powder is added.

[0071] A stomach-nourishing and spleen-strengthening composite probiotic powder comprises the following components in parts by weight: 10 parts of freeze-dried bacterial powder, 45 parts of prebiotics, 22 parts of fruit and vegetable powder, 29 parts of enzymatically hydrolyzed plant powder and 53 parts of sugar alcohol.

[0072] Comparative Example 3

[0073] The difference between this comparative example and Example 1 is that the types of bacteria in the freeze-dried powder are different.

[0074] The freeze-dried bacterial powder is prepared by mixing lactobacillus acidophilus, bifidobacterium animalis, lactobacillus plantarum, bifidobacterium lactis and lactobacillus rhamnosus.

[0075] The number of live Lactobacillus acidophilus in the freeze-dried bacterial powder is 5×10 9 CFU / g, the number of live Bifidobacterium animalis 6×10 8 CFU / g, number of live Lactobacillus plantarum 1×10 9 CFU / g, the number of live Bifidobacterium lactis is 10×10 9 CFU / g and the number of live Lactobacillus rhamnosus bacteria 2×10 9 CFU / g.

[0076] Comparative Example 4

[0077] The difference between this comparative example and Example 1 is that the concentration of bacteria in the freeze-dried powder is different.

[0078] The number of live Lactobacillus acidophilus in the freeze-dried bacterial powder is 1×10 9 CFU / g, the number of live bacteria of animal Bifidobacterium 10×10 8 CFU / g, number of live Lactobacillus plantarum 5×10 9 CFU / g, the number of live Lactobacillus helveticus is 26×10 9 CFU / g and the number of live Lactobacillus bulgaricus is 6×10 9 CFU / g.

[0079] Comparative Example 5

[0080] The difference between this comparative example and Example 1 is that the weight ratio of strawberry, mulberry and raspberry is 1:1:1.

[0081] Comparative Example 6

[0082] The difference between this comparative example and Example 1 is that cellulase is replaced by ficin; pectinase is replaced by bromelain.

[0083] Comparative Example 7

[0084] The difference between this comparative example and Example 1 is that the weight ratio of garcinia cambogia, blueberry and cherry is 1:1:1.

[0085] Comparative Example 8

[0086] The difference between this comparative example and Example 1 is that Bifidobacterium breve is replaced by Lactobacillus breve; and Lactobacillus casei is replaced by Bifidobacterium longum.

[0087] Comparative Example 9

[0088] The difference between this comparative example and Example 1 is that the weight ratios of the freeze-dried bacterial powder, prebiotics and sugar alcohol are different.

[0089] A stomach-nourishing and spleen-strengthening composite probiotic powder comprises the following components in parts by weight: 6 parts of freeze-dried bacterial powder, 41 parts of prebiotics, 22 parts of fruit and vegetable powder, 16 parts of enzymatic plant powder, 13 parts of fermented plant powder and 61 parts of sugar alcohol.

[0090] (I) Performance test

[0091] 1. Clinical Cases

[0092] A total of 550 patients with symptoms such as epigastric pain, abdominal distension, vomiting, belching, constipation, etc., aged between 18 and 65 years old, voluntarily underwent follow-up treatment and were randomly divided into 11 groups, 50 people in each group, and took the composite probiotic powder prepared in Examples 1-2 and Comparative Examples 1-9 respectively.

[0093] 2. Treatment methods

[0094] Usage and treatment course: Take the composite probiotic powder prepared in Examples 1-2 of the present invention and Comparative Examples 1-9 with water half an hour before meals, 3 times a day, 8g each time. Do not eat raw, cold, spicy and other irritating foods during the medication period. 7 days is a course of treatment.

[0095] 3. Efficacy judgment

[0096] Recovery: Symptoms such as epigastric pain, abdominal distension, vomiting, belching, and constipation completely disappeared; appetite increased, and there was no discomfort after eating;

[0097] Improvement: Symptoms such as epigastric pain, abdominal distension, vomiting, belching, and constipation have been alleviated, but there is still a slight loss of appetite;

[0098] Ineffective: Symptoms such as epigastric pain, abdominal distension, vomiting, belching, and constipation were not improved.

[0099] 4. Treatment results

[0100] After three courses of treatment, the number of people who were cured, improved, or ineffective was counted. A follow-up visit was conducted one year later to count the number of people who relapsed among the cured people.

[0101] The results are shown in Table 1.

[0102] Table 1 Performance test results

[0103]

[0104]

[0105] (II) Storage stability test

[0106] The composite probiotic powders prepared in Examples 1-2 and Comparative Examples 1-9 were sealed and stored, and placed in the following four environments for observation to test the storage stability.

[0107] Normal temperature: The sample is placed at 25°C, 60% RH (relative humidity) for 180 days.

[0108] Cold resistance: -10℃ for 30 days.

[0109] Hot and cold cycle: -10℃, 25%RH; 25℃, 60%RH; 38℃, 75%RH, 24h at each temperature, 30 cycles in total.

[0110] High temperature acceleration: The test conditions are 50℃±1℃, 25%RH±5%RH for 90 days.

[0111] If there is no obvious change, it is recorded as passed; if one or more of the following changes, such as agglomeration, odor, or color change, is observed, it is recorded as failed.

[0112] The results are shown in Table 2.

[0113] Table 3 Stability test results

[0114]

[0115]

[0116] It can be seen from the above performance test results that the composite probiotic powders of Examples 1-2 have good stomach-nourishing and spleen-strengthening effects, especially the comprehensive performance of Example 1 is the most outstanding, which is mainly due to the synergistic effect of multiple components.

[0117] However, the comparative examples are significantly worse than the embodiments in the corresponding performance tests because they do not adopt the necessary technical solutions. In comparative example 1, the composition ratio of the composite probiotic powder is changed, and it can be seen that the therapeutic effect decreases. In comparative example 2, the fermented plant powder solution is added, and the results show that the curative effect is not ideal. The types or addition amounts of bacteria in the freeze-dried bacterial powders of comparative examples 3-4 are different, which affects the curative effect. The preparation methods of the enzymatic hydrolyzed plant powder and the fermented plant powder of comparative examples 5-8 are different, and the recurrence rate after recovery becomes higher. The weight ratios of freeze-dried bacterial powder, prebiotics and sugar alcohols in comparative example 9 are different, and the stability of the composite probiotic powder decreases. The above experimental results further prove the importance of the technical solution defined in the present invention for its technical effect.

[0118] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composite probiotic powder for nourishing the stomach and spleen, characterized in that: The invention comprises the following components in parts by weight: 6-12 parts of freeze-dried bacterial powder, 40-50 parts of prebiotics, 20-25 parts of fruit and vegetable powder, 14-19 parts of enzymatic plant powder, 12-15 parts of fermented plant powder and 46-61 parts of sugar alcohol; the freeze-dried bacterial powder is a mixture of Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus plantarum, Lactobacillus helveticus and Lactobacillus bulgaricus; the prebiotics are a mixture of inulin, galacto-oligosaccharide, isomaltooligosaccharide and fructo-oligosaccharide in a weight ratio of 10:(8-12):(4-6):(13-16).

2. The stomach-nourishing and spleen-strengthening composite probiotic powder according to claim 1, characterized in that: The sugar alcohol is a mixture of lactitol, erythritol and xylitol in a weight ratio of 1: (1.3-1.5): (0.4-0.7).

3. The stomach-nourishing and spleen-strengthening composite probiotic powder according to claim 1, characterized in that: The fruit and vegetable powder comprises the following raw materials in parts by weight: 2-6 parts of apple powder, 11-14 parts of banana powder, 4-10 parts of papaya powder, 5-8 parts of pitaya powder and 6-12 parts of pumpkin powder.

4. The stomach-nourishing and spleen-strengthening composite probiotic powder according to claim 1, characterized in that: The number of live Lactobacillus acidophilus in freeze-dried bacterial powder is 3.0×10 9 CFU / g-6.0×10 9 CFU / g; The number of live Bifidobacterium animalis 5.0×10 8 CFU / g-8.0×10 8 CFU / g; The number of viable Lactobacillus plantarum cells was 0.8×10 9 CFU / g-2.0×10 9 CFU / g; The number of live Lactobacillus helveticus cells was 8.0×10 9 CFU / g-11.0×10 9 CFU / g; The number of live Lactobacillus bulgaricus cells was 1.0×10 9 CFU / g-3.0×10 9 CFU / g.

5. The stomach-nourishing and spleen-strengthening composite probiotic powder according to claim 1, characterized in that: The weight ratio of freeze-dried bacterial powder, prebiotics and sugar alcohol is 10:(43-46):(51-54).

6. The stomach-nourishing and spleen-strengthening composite probiotic powder according to claim 1, characterized in that: The preparation method of the enzymatic hydrolysis plant powder comprises the following steps: mixing fresh strawberries, mulberries and raspberries, draining the surface water, crushing them, adding water to obtain a mixed solution A, adding cellulase, performing enzymatic hydrolysis at 40-45° C. for 4-6 hours, and inactivating the enzyme; continuously adding pectinase, performing enzymatic hydrolysis at 40-45° C. for 3-5 hours, inactivating the enzyme, filtering, drying, crushing and sieving to obtain the enzymatic hydrolysis plant powder.

7. The stomach-nourishing and spleen-strengthening composite probiotic powder according to claim 6, characterized in that: The enzymatic activity of cellulase in the mixed solution A is 80U / mL-100U / mL; the enzymatic activity of pectinase in the mixed solution A is 140U / mL-160U / mL.

8. The stomach-nourishing and spleen-strengthening composite probiotic powder according to claim 1, characterized in that: The preparation method of the fermented plant powder comprises the following steps: mixing fresh garcinia cambogia, blueberry and cherry, draining the surface water, crushing and adding water to obtain a mixed solution B, adding Bifidobacterium breve, fermenting at 40-45° C. for 1-3 days, and sterilizing; continuously adding Lactobacillus casei, fermenting at 40-45° C. for 3-6 days, sterilizing, filtering, drying, crushing and sieving to obtain the fermented plant powder.

9. The stomach-nourishing and spleen-strengthening composite probiotic powder according to claim 8, characterized in that: The bacterial activity of Bifidobacterium breve in mixed solution B is 10 10 CFU / mL-10 11 CFU / mL; the bacterial activity of Lactobacillus casei in mixed solution B is 10 8 CFU / mL-10 9 CFU / mL.

10. The method for preparing the stomach-nourishing and spleen-strengthening composite probiotic powder according to any one of claims 1 to 9, characterized in that: The following steps are involved: Prebiotics, fruit and vegetable powder, enzymatic plant powder, fermented plant powder and sugar alcohol are mixed, sprayed with alcohol to make them bond and granulate, and then mixed with freeze-dried bacterial powder to obtain composite probiotic powder.

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