The invention relates to the technical field of food, and discloses a preparation method of freeze-dried sterilized yoghourt, which comprises the following steps: (1) mixing and heating raw materials; the raw materials comprise fermented milk,
calcium caseinate, composite proteolytic
enzyme,
starch and water; (2) maintaining the material temperature at 45-60 DEG C, and performing enzymolysis; (3) raising the temperature to 80-95 DEG C, maintaining the temperature for 20-40 minutes, and performing
enzyme deactivation; and (4) after cooling, freezing the material liquid until the material temperature reaches-25 DEG C or below, and
drying the frozen material liquid in a freeze-
drying bin to obtain the freeze-dried sterilized yoghurt. The enzymolysis step is added before the yogurt is sterilized, and the
protein is slightly hydrolyzed by adopting the composite proteolytic
enzyme, so that the problem that the taste and
flavor of the existing
pasteurization sterilized yogurt are degraded due to high-
temperature treatment is solved, the production process is simplified, the cost is reduced, and the production efficiency is improved. Finally, the freeze-dried sterilized yoghourt product with natural
flavor and good taste is obtained.