The invention provides a method for preparing mask base materials. The method includes steps of acquiring coconut water, adding xylan and pectin into the coconut water, carrying out pasteurization, then inoculating acetobacter xylinum into the coconut water, cultivating the acetobacter xylinum until fibrous membranes with the thicknesses of 0.1-0.5 cm are formed on the surface of fermentation liquor, adding cellulase into the fibrous membranes, treating the fibrous membranes, then carrying out ultrasonic treatment on the fibrous membranes in sodium hydroxide solution, washing the fibrous membranes by the aid of clear water until the fibrous membranes are neutral, slicing the fibrous membranes and then carrying out freeze drying on the fibrous membranes to obtain cellulose substrates; acquiring streptococcus zooepidemicus fermentation liquor containing hyaluronic acid, centrifugally removing precipitates, adding supernate into albumen powder, uniformly mixing the supernate and the albumen powder with each other to obtain mixtures, then adding the cellulose substrates into the mixtures, carrying out ultrasonic treatment, taking out the cellulose substrates after ultrasonic treatment is completely carried out, thorough flushing the mixtures by the aid of clear water, carrying out freeze drying on the mixtures and then packaging the mixtures under vacuum conditions to obtain the mask base materials. The method has the advantages that the mask base materials prepared by the aid of the method are excellent in human skin compatibility, free of skin irritation and suitable for various skin types; the mask base materials contain abundant hyaluronic acid, the hyaluronic acid is uniformly distributed, excellent slow-release effects can be realized, the mask base materials are long in skin retention time and are nutritional, and moisture can be preserved.