The invention provides a chromium (III) complex as well as a preparation method and application thereof. According to the chromium (III) complex, the molecular formula is CrC14H26N4O4Cl, the molecular weight is 402, the crystal structure belongs to a triclinic crystal system, the spatial point group is P-1, a is equal to 7.8880(6), b is equal to 9.8380(7), c is equal to 14.4490(12) angstroms, alpha is equal to 70.4550(10), beta is equal to 85.729(2), gamma is equal to 82.0880(10) degrees, V is equal to 1046.12(14) cubic angstroms, and Z is equal to 2. The preparation method comprises the steps of dissolving chromium (III) and 3-methyl-salicylic acid by virtue of an organic solvent, feeding a small amount of zinc granules, carrying out heating reflux, then dropwise adding triethylene tetramine, continuing to carry out reflux for a period of time, stopping reaction, cooling, filtering, standing obtained filtrate, and naturally volatilizing at the normal temperature, so as to obtain purple red crystals. The chromium (III) complex can be applied to the preparation of sugar-reducing medicines.