The invention discloses a preparation method of lentinus edodes soup. The preparation method is characterized in that the lentinus edodes soup comprises the following ingredients in percent by mass: dry lentinus edodes powder, sodium glutamate, disodium 5'-ribonucleotide, denaturated starch, refined edible salt, white granulated sugar and white pepper powder; the preparation method of the lentinus edodes soup comprises the following steps of: step A, selecting high-quality dry lentinus edodes powder, sodium glutamate, disodium 5'-ribonucleotide, denaturated starch, refined edible salt, white granulated sugar and white pepper powder in percent by mass, and respectively grinding into powder; and step B, putting the grinded powder into a special stirrer container, adding an appropriate amount of concentrated soup-stock and uniformly stirring for later use, subsequently pelleting the uniformly stirred article by using a special pelletizer, and drying and screening, packaging and sealing so as to obtain the lentinus edodes soup. The lentinus edodes soup is economic and practical, low in cost and good in seasoning effect, can be widely used in noodles, snack food, boiled dumplings, wonton, various cold vegetable dishes in sauce and vegetable soup.