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Lactobacillus acidophilus composite freeze-drying protective agent and preparation method and usage method thereof

A freeze-drying protective agent, Lactobacillus acidophilus technology, applied in biochemical equipment and methods, microorganism-based methods, bacteria and other directions, can solve the problems of freeze-dried bacteria powder easy to absorb water and moisture, low survival rate, inconvenient to use and so on , to achieve the effect of simple preparation method and use method, improving survival rate and prolonging storage period

Inactive Publication Date: 2012-10-31
ENZYME ENG INST SHAANXI PROVINCE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of Lactobacillus acidophilus compound freeze-drying protective agent and its preparation and use method, no milk component and glycerin in this protective agent, solve the viable count of Lactobacillus acidophilus bacterial powder in the freeze-drying process Low, low survival rate, freeze-dried bacteria powder is easy to absorb water and moisture, and it is inconvenient to use in preparations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] First weigh 7g lactose, 11g galactose, 8.5g trehalose, 1.3g Na 2 HPO 4 and 0.4g MgSO 4 , dissolved in 180mL of distilled water by heating, sterilized at 110°C for 15min, and cooled to room temperature; then dissolved 2g of sodium ascorbate in 20mL of sterile saline, and sterilized by filtration with a 0.22μm sterile filter membrane; then the filtrate was mixed with the cooled The solution is mixed evenly according to the requirements of aseptic operation to obtain the compound freeze-drying protective agent.

[0016] After the Lactobacillus acidophilus has been activated and checked for no miscellaneous bacteria and abnormal morphology, it is inserted into the MRS medium at an inoculation amount of 4% (v / v), the fermentation temperature is 37°C, and the facultative anaerobic fermentation is cultivated for 18 hours Finally, centrifuge at 6000r / min for 10min to obtain Lactobacillus acidophilus sludge; add the above-mentioned freeze-drying protective agent at a volume ra...

Embodiment 2

[0019] First, 8.5g lactose, 12g galactose, 10g trehalose, 1.2g Na 2 HPO 4 and 0.6g MgSO 4 , heated and dissolved in 185mL distilled water, sterilized at 110°C for 15min, and cooled to room temperature; then dissolved 1.7g sodium ascorbate in 15mL sterile normal saline, and sterilized by filtration with a 0.22μm sterile filter membrane; then the filtrate was mixed with the cooled The solution is mixed evenly according to the requirements of aseptic operation to obtain the compound freeze-drying protective agent.

[0020] According to the method of Example 1, the Lactobacillus acidophilus sludge was obtained, and the above-mentioned freeze-drying protective agent was added at a volume ratio of 1:2, shaken and left for 30 minutes, pre-frozen in a low-temperature refrigerator at -40°C for 6 hours, and then placed in a freeze dryer. Freeze-dry at 4-6 Pa for 18 hours to obtain Lactobacillus acidophilus powder with protective agent.

[0021] After testing, the freeze-dried surviva...

Embodiment 3

[0023] First, 11g lactose, 15g galactose, 12g trehalose, 1.5g Na 2 HPO 4 and 0.8g MgSO 4 , dissolved in 180mL of distilled water by heating, sterilized at 110°C for 15min, and cooled to room temperature; then dissolved 2.5g of sodium ascorbate in 20mL of sterile saline, and sterilized by filtration with a 0.22μm sterile filter membrane; The final solution is mixed evenly according to the requirements of aseptic operation to obtain the compound freeze-drying protective agent.

[0024] According to the method of Example 1, Lactobacillus acidophilus bacteria slime was obtained; the above-mentioned freeze-drying protective agent was added in a volume ratio of 1:2, shaken and left for 30 minutes, pre-frozen in a low-temperature refrigerator at -40 ° C for 8 hours, and placed in a freeze dryer Freeze-dry at 4-6Pa for 24 hours to obtain Lactobacillus acidophilus powder with protective agent.

[0025] After testing, the freeze-dried survival rate was 92.68%, and the number of live ...

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Abstract

The invention discloses a lactobacillus acidophilus composite freeze-drying protective agent and a preparation method and a usage method thereof. The protective agent comprises lactose, galactose, trehalose, Na2HPO4, sodium ascorbate and MgSO4. Compared with the prior art, the protective agent don not contain glycerol, so that the livability (above 85%) and the stability of freeze drying preservation of the lactobacillus acidophilus, the storage period (the period is 1 year, mycelia powder viable count is more than 1011cfu / g) of the lactobacillus acidophilus is prolonged, and no milk composition is included, and the agent can be used widely in non-dairy products. The preparation method and the usage method are simple, easy to operate and low in cost.

Description

technical field [0001] The invention belongs to the technical field of biological bacteria products, and in particular relates to a compound freeze-drying protectant for preparing highly active Lactobacillus acidophilus bacteria powder and a preparation and use method thereof. Background technique [0002] Lactobacillus acidophilus (Lactobacillus acidophilus) is a Lactobacillus genus, Gram-positive, non-spore-forming, elongated bacilli without flagella, capsules, and spores. It does not move, exists in single, double or short chains, and can ferment. Sugar-producing lactic acid, ethanol, hydrogen peroxide and other anaerobic or facultative anaerobic microorganisms can use glucose, fructose, lactose, and sucrose for homofermentation to produce DL-type lactic acid. [0003] At present, the preparation of Lactobacillus acidophilus powder mainly adopts vacuum freeze-drying technology, but if Lactobacillus acidophilus is directly freeze-dried, the survival rate is extremely low, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12R1/23
Inventor 秦涛马齐陈合舒国伟付博
Owner ENZYME ENG INST SHAANXI PROVINCE ACAD OF SCI
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