Double-stranded RNA capable of preventing and controlling mosquitoes, expression vector and applications of double-stranded RNA and expression vector

A technology of expressing vectors and vectors, applied in the field of recombinant vectors and their applications, and double-stranded RNA, which can solve the problems of ineffective inhibition of HR3 expression, larvae death, and inability of insects to molt normally, and achieve high commercial application value

Active Publication Date: 2019-07-23
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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Problems solved by technology

Inhibition of HR3 expression prevents insects from molting normally and eventually leads to larval death
However, there is no means to effectively inhibit the expression of HR3

Method used

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  • Double-stranded RNA capable of preventing and controlling mosquitoes, expression vector and applications of double-stranded RNA and expression vector
  • Double-stranded RNA capable of preventing and controlling mosquitoes, expression vector and applications of double-stranded RNA and expression vector
  • Double-stranded RNA capable of preventing and controlling mosquitoes, expression vector and applications of double-stranded RNA and expression vector

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Embodiment Construction

[0033]The present invention will be further described below in conjunction with specific examples, so as to better understand the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores. Markers were purchased from Dalian Bao Biological Company. % in the following examples, unless otherwise specified, are mass percentages. Quantitative experiments in the following examples were all set up to repeat the experiments three times, and the results were averaged.

[0034] 1. Construction of Aedes mosquito HR3 RNAi expression vector

[0035] (1) Obtaining of HR3 RNAi forward and reverse fragments

[0036] Dalian Bao Biological Engineering Co., Ltd. chemically synthesized the HR3 gene fragment, cloned it into the pUC57 vector, and named it pUC57-HR3. The pUC57-HR3 vector was am...

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Abstract

The invention provides a double-stranded RNA capable of preventing and controlling mosquitoes, an expression vector and applications of the double-stranded RNA and the expression vector. The double-stranded RNA consists of a sense strand and an antisense strand: the nucleotide sequence of the sense strand is as shown in SEQ ID NO.2 in a sequence table; and the nucleotide sequence of the antisensestrand is as shown in SEQ ID NO.3 in the sequence table. The invention also provides the expression vector containing the double-stranded RNA and an application thereof. The double-stranded RNA and the expression vector containing the double-stranded RNA provided by the invention can effectively silence the HR3 gene, can be used for biological control of the aedes, and has environment-friendly characteristics and higher commercial application value. For example, in a specific embodiment, the expression vector containing the RNA transforms chlamydomonas and chlorella to obtain an engineering algae strain, the aedes larvae are fed with the engineering algae strain, and the aedes larvae can be effectively killed.

Description

technical field [0001] The invention relates to a double-stranded RNA, a recombinant vector and applications thereof, in particular to a double-stranded RNA capable of preventing and controlling mosquitoes, an expression vector and applications thereof. Background technique [0002] Dengue fever (Dengue fever, DF) is caused by dengue virus (Dengue virus), a kind of insect-borne infectious disease characterized by fever, joint pain, and red rash. Infection with dengue virus can cause dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. According to WHO statistics, there are about 100 million infected people every year, of which 500,000 are hospitalized, and 20,000 patients die from severe dengue fever or dengue hemorrhagic fever. Dengue fever is mainly distributed in tropical and subtropical regions, and its incidence is increasing year by year. Since my country first reported the dengue fever epidemic in Foshan City, Guangdong Province in 1978, it has also be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/63C12N15/66C12N1/13A01N57/16A01N63/00A01P7/04C12R1/89
CPCC12N15/113C12N15/63C12N15/66A01N57/16A01N63/00C12N2310/14
Inventor 邓晓东费小雯
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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