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Nano contrast agent for diagnosis and treatment integration and preparation method thereof

A nano-contrast agent, contrast agent technology, applied in the direction of nanotechnology for materials and surface science, general/multifunctional contrast agents, chemical instruments and methods, etc., can solve the problem of lack of chemical dynamic therapy function, etc., to achieve the improvement of tumor Healing effect, effect of high absorption coefficient

Active Publication Date: 2022-03-29
THE FIRST AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention intends to provide a nano-contrast agent for the integration of diagnosis and treatment to solve the lack of technologies in the prior art that have photothermal, photodynamic, chemodynamic therapy functions and multiple diagnostic methods integrated nano-drug delivery systems or contrast agents question

Method used

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  • Nano contrast agent for diagnosis and treatment integration and preparation method thereof
  • Nano contrast agent for diagnosis and treatment integration and preparation method thereof
  • Nano contrast agent for diagnosis and treatment integration and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: the preparation of nano-contrast agent

[0050] 1. Take the mixture I composed of dibenzyl ether, 1,2-dodeynediol and hexacarbonyl tungsten, place it in a three-necked flask with vigorous magnetic stirring, and heat it up under the protection of nitrogen. The dosage ratio of dibenzyl ether, 1,2-dodeynediol and hexacarbonyl tungsten is 20ml: 1.5g: 1mmol.

[0051] 2. When the temperature of the system is heated to 120°C, add oleic acid and oleylamine (the volume ratio of oleic acid, oleylamine and dibenzyl ether is 1:1:20) to the mixed solution I to obtain the mixed solution II, and then continue Heat up.

[0052] 3. When the temperature of the mixed solution II rises to 260°C, then add ferrous acetylacetonate to the mixed solution II, and continue the reaction for 30 minutes to obtain the mixed solution III. Among them, the molar ratio of tungsten hexacarbonyl to ferrous acetylacetonate is 1:1.

[0053] The entire process of steps 1-3 needs to be isolate...

Embodiment 2

[0063] Example 2: Photothermal properties of nano-contrast agents

[0064] The photothermal properties of nanocontrast agents were evaluated by laser irradiation of different concentrations of FWO-PEG NSs aqueous solution (0, 50, 100, 200, 400 μg / mL), and the temperature in the liquid was monitored by IVIS spectral imaging system, and strictly Record. For experimental results, see Figure 12 , different concentrations of FWO-PEG NSs were placed in a 96-well plate, at 1060nm (1.0W / cm 2 ) under laser irradiation, the temperature of the solution at different time points was recorded. With the increase of the irradiation time, the solution temperature gradually increased, and the higher the concentration, the faster the heating rate, indicating the excellent photothermal conversion performance of FWO-PEG NSs .

Embodiment 3

[0065] Embodiment 3: the photodynamic property of nano-contrast agent

[0066]The photodynamic properties of nano-contrast agents were detected by DPBF (diphenylisobenzofuran) method. FWO-PEG NSs and DPBF were mixed and added to PBS buffer (pH 6.0) (500 μg / mL of FWO-PEG NSs and 15 μL of 1 mg / mL DPBF were mixed in 1 mL of PBS system), and then different Time (0, 5, 10, 15, 20, 25 and 30 min). And use the UV spectrophotometer to observe the absorbance change at 418nm. For experimental results, see Figure 13 (The curves in the figure represent the test results with irradiation time of 0, 5, 10, 15, 20, 25 and 30 minutes from top to bottom), 1 o 2 It can specifically react with the reagent DPBF to reduce its characteristic peak. System at 1060nm (0.5W / cm 2 ) under different time of laser irradiation, the characteristic peak of DPBF at 418nm gradually decreased, which proves that with the extension of irradiation time, the 1 o 2 The increase in output is positively correla...

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Abstract

The invention relates to the technical field of nano-drug presentation systems and contrast agents, in particular to a nano-contrast agent for diagnosis and treatment integration and a preparation method of the nano-contrast agent. The nanometer contrast agent for diagnosis and treatment integration comprises an iron-tungsten oxide nanosheet, and the mass ratio of the iron element to the tungsten element is 1: 1.13. According to the technical scheme, a tungsten source and an iron source are subjected to a high-temperature reaction under the conditions that dibenzyl ether serves as a solvent, 1, 2-dodecynediol serves as a reducing agent and oleic acid oleylamine serves as a surfactant, iron tungsten oxide nanosheets FWO NSs are obtained, surface PEG modification is further conducted, and the FWO-PEG NSs with good water solubility is obtained. The nanosheet can realize photothermal, photodynamic and chemical dynamic therapy, can also realize monitoring of MRI / CT / PA triple imaging, is a nano-drug presentation system and a contrast agent with medical imaging means for real-time monitoring and regulation, and has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of nano-medicine delivery system and contrast agent, in particular to a nano-contrast agent for integrated diagnosis and treatment and a preparation method thereof. Background technique [0002] Phototherapy (PDT and PTT) is a kind of phototherapy mediated by photosensitizers. Under the external laser, the photothermal effect of heating and the photodynamic effect of toxic reactive oxygen species are respectively used to effectively kill tumor cells. Due to its minimally invasive, non-toxic Invasiveness and other advantages have been successfully applied in clinical practice. However, photothermal therapy leads to tumor resistance, and photodynamic therapy is affected by the hypoxic environment in the tumor. The single treatment method has an unsatisfactory anti-tumor effect. Alternatively, chemodynamic therapy (CDT) exploits the response of certain substances to low pH and abundant H 2 o 2 The level of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/60A61K33/26A61K49/12A61K49/22A61P35/00B82Y5/00B82Y30/00B82Y40/00C01G49/00
CPCA61K47/60A61K41/0052A61K41/0057A61K33/26A61P35/00A61K49/126A61K49/225A61K49/0002C01G49/00B82Y5/00B82Y30/00B82Y40/00C01P2002/72C01P2002/85C01P2004/04C01P2004/20A61K2300/00Y02A50/30
Inventor 向覃言秋王志刚罗远利杨超
Owner THE FIRST AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIVERSITY
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