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127results about How to "High commercial application value" patented technology

Iota-carrageenan degrading bacterium as well as fermentation method and application thereof

The invention provides an iota-carrageenan degrading bacterium as well as a fermentation method and an application thereof, and relates to cytophaga bacteria. The collection number of the iota-carrageenan degrading bacterium (Cellulophaga sp.) KL-A is CGMCC No. 8694. A production method of iota-carrageenan hydrolytic enzyme comprises the following steps: coating an iota-carrageenan solid medium with the activated iota-carrageenan degrading bacterium (Cellulophaga sp.) KL-A, picking and inoculating a single colony into a seed medium and culturing so as to obtain a seed solution; and inoculating the seed solution into fermentation liquid according to 1%-5% of inoculation quantity, and carrying out shake cultivation so as to obtain fermented supernatant; and carrying out salt fractionation by using ammonium sulfate and DEAE (diethyl-aminoethanol)-cellulose chromatography on the obtained fermented supernatant at the temperature of 4 DEG C, dialyzing so as to remove salt, and carrying out vacuum freeze drying so as to obtain the iota-carrageenan hydrolytic enzyme. Iota-carrageenan oligosaccharides can be applied in the preparation of antibacterial agents, antiviral drugs, immunomodulators, antioxidants and the like.
Owner:THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION

SSR primers and method for purity identification of luffa hybrid seeds

The invention provides SSR primers and method for purity identification of luffa hybrid seeds. The primers comprise an upstream primer SGSSR4-F of a primer SGSSR4 and a downstream primer SGSSR4-R of the primer SGSSR4, wherein the nucleotide sequence of the upstream primer SGSSR4-F is as shown in SEQ ID NO. 1, and the nucleotide sequence of the downstream primer SGSSR4-R is as shown in SEQ ID NO.2. The method includes the following steps that a luffa parent and a hybrid progeny genome DNA are extracted; the hybrid progeny genome DNA serves as a template, and the primer SGSSR 4 is utilized to conduct PCR amplification; an amplification product is subjected to gel electrophoresis; an electrophoresis result is analyzed, only a single plant with parent specificity bands at the same time becomes a real hybrid seed, the plant in lack of any band is marked as a false hybrid seed, and seed purity is calculated; the primer SGSSR4 can generate 151bp male parent specificity bands and 135bp female parent specificity bands. The primers can generate male parent specificity marks and female parent specificity marks, and specificity is high; by means of the SSR primers, purity of the luffa hybrid seeds can be identified rapidly, conveniently, accurately and effectively.
Owner:CROP RES INST OF FUJIAN ACAD OF AGRI SCI

Nickel-cobalt-iron ternary oxide water electrolysis composite material, preparation method and application thereof

ActiveCN112619649AOptimizing the electrolytic water reaction pathRetain the porous structureHeterogenous catalyst chemical elementsMetal/metal-oxides/metal-hydroxide catalystsElectrolysisNew energy
The invention provides a nickel-cobalt-iron ternary oxide water electrolysis composite material, a preparation method and application thereof, the composite material is composed of nickel-cobalt-iron ternary oxide grown on foamed nickel in situ, the expression of the composite material is R-NiCoFeO@NF, and the invention belongs to the technical field of new energy material synthesis. A nickel-cobalt-iron ternary prussian blue analog growing on foamed nickel in situ is adopted as the template, the nickel-cobalt-iron ternary oxide with an electronic structure optimized inverse spinel structure is obtained through high-temperature calcination in air and subsequent hydrogen heat treatment. According to the synthesis method, the microstructure of the nickel-cobalt-iron ternary oxide can be effectively controlled, the electronic structure of the nickel-cobalt-iron ternary oxide is adjusted, and the regulation and control method of the electronic structure of the nickel-cobalt-iron ternary oxide is enriched. Meanwhile, the composite material also shows excellent electrocatalytic water decomposition activity, the overpotential of the composite material is only 245mV when the current density is 10mAcm<-2>, and the composite material is suitable for the field of new energy development.
Owner:CHINA UNIV OF PETROLEUM (EAST CHINA)

Berberine hydrochloride eutectic crystal and preparation method thereof

The invention relates to a berberine hydrochloride eutectic crystal and a preparation method thereof. The method comprises the steps that: berberine hydrochloride and diacid are dissolved in a solvent, then cooling crystallization or standing crystallization is adopted, and a separated crystal is the berberine hydrochloride eutectic crystal; the berberine hydrochloride eutectic crystal is a berberine hydrochloride-diacid eutectic crystal of a basic structural unit formed by taking the berberine hydrochloride as an API and the diacid as a CCF; and the berberine hydrochloride-diacid eutectic crystal is a triclinic system and a P1 space group. According to the berberine hydrochloride eutectic crystal and the preparation method thereof of the invention, by means of the eutectic crystal mode, the powder dissolution rate and the inherent dissolution rate of the berberine hydrochloride are improved, and the hygroscopicity of the berberine hydrochloride is reduced, the berberine hydrochloridehas better stability, the bioavailability of the berberine hydrochloride can be greatly improved, and the curative effect of the berberine hydrochloride can be better exerted. In addition, the preparation method of the eutectic crystal is simple and easy to implement, mild in condition, easy to control, good in reproducibility and low in production cost, and has a great commercial application value; and the large-scale industrial production of the eutectic crystal is easy to achieve.
Owner:SHANGHAI UNIV OF ENG SCI
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