The invention discloses a method for detecting
protein based on
graphene sensor. The method comprises the following steps: preparing a
graphene sensor, namely arranging a
graphene thin film on a substrate, and arranging electrodes at the two ends of the graphene thin film; (2) pretreating a sample to be detected, namely adding fluorescent dyes which are used for labeling
protein in the sample into the sample to be detected, thus obtaining a fluorescent dye-
protein compound; and (3) detecting the content of protein in the sample, namely dropwise adding pretreated sample to be detected to the surface of the graphene thin film, and
quenching fluorescence under the
irradiation of
ultraviolet light, wherein the graphene thin
film resistance is changed, the variable quantity of the resistance is in direct proportion to the content of the fluorescent dye-protein compound, so that the content of protein in the sample is detected. According to the method, the protein can be detected with high sensitivity and high precision, and compared with the conventional
fluorescence excitation detection method, the method has the advantages of simplicity and rapid measurement speed.