Tumor tissue transport fluid and application thereof

A technology of tumor tissue and transport fluid, applied in the biological field, can solve the problems of lack of nutrients in tumor cells, reduced success rate of PDX membrane construction, and reduced tissue activity, etc., to achieve high commercial application value, protect cell morphology, and improve the lack of nutrients Effect

Inactive Publication Date: 2020-10-30
江苏莱森生物科技研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the common tumor tissue preservation solution in vitro has simple components, mainly basal medium, antibiotics, etc., which lack the rich nutrients necessary for tumor cell growth and metabolism, resulting in reduced tissue activity and prone to contamination, resulting in a lower success rate of PDX membrane construction. , and the cost is high and the experiment is difficult

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Configuration of tumor tissue transport solution

[0017] In the present invention, DMEM high-glucose medium is used as the basic medium for maintaining tissue activity; insulin (Insulin) is used for maintaining the uptake and utilization of glucose by tissue cells; thyrotropin-releasing factor (TRF) is also known as stimulating Thyroid hormone-releasing hormone is used to maintain the activity of tissue cells; cortisol is a type of glucocorticoid used to maintain the metabolism of sugar, lipid and protein in tissue cells, and transferrin is added to carry iron released by red blood cell degradation to maintain cell Metabolism; adding glutamine and non-essential amino acids is mainly to supplement the amino acids needed for tissue cell metabolism; adding sodium selenite to supplement trace elements; adding penicillin, streptomycin, gentamicin, and amphotericin antibiotics are In order to prevent the tissue from being bio-contaminated by bacteria during the tr...

Embodiment 2

[0019] Example 2: Preparation of tumor tissue transport solution

[0020] Take a 50ml sterile centrifuge tube, add 0.5 mg / ml insulin, 1mM TRF, 100unit / ml penicillin, 100μg / ml streptomycin, 50 mg / ml gentamicin, 0.25 mg / ml The tumor tissue transfer solution was prepared by adding ml amphotericin, 0.5% non-essential amino acids, 0.5% L-glutamine, 18 ng / ml cortisol, 0.1 mg / ml transferrin and 0.1 μg / ml sodium selenite.

Embodiment 3

[0021] Example 3: Configuration of tumor tissue transport solution

[0022] Take a 50ml sterile centrifuge tube, add 1 mg / ml insulin, 6 mM TRF, 100unit / ml penicillin, 100μg / ml streptomycin, 50 mg / ml gentamicin, 0.25 mg / ml amphotericin, 2% non-essential amino acids, 2% L-glutamine, 30 ng / ml cortisol, 0.4 mg / ml transferrin and 0.6 μg / ml sodium selenite to prepare tumor tissue transfer solution .

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PUM

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a tumor tissue transport fluid and an application thereof. The tumor tissue transport fluid provided by the invention comprises a plurality of antibiotics and nutritional factors, is definite in components, does not contain serum, can significantly improve the defect of nutrient depletion of previous tissue transport fluids, and avoids activity reduction of tumor samples in storage or transportation to a great extent. The transport liquid provided by the invention is simple to prepare, can effectively improvethe activity of a fresh tumor sample, can well protect the cellular morphology of the tumor sample, can significantly increase the modeling rate of PDX, shortens the modeling time, has great help forestablishment of a PDX tumor model and has a high commercial application value.

Description

technical field [0001] The invention belongs to the field of biological technology, and specifically relates to a tumor tissue transport liquid and its application. Background technique [0002] PDX (Patient-Derived tumor Xenograft) human-derived tumor xenograft model refers to a tumor model established by transplanting the tumor tissue of a tumor patient into an immunodeficient mouse and relying on the microenvironment provided by the mouse for growth. The PDX model retains the patient's tumor tissue, retains the histological and genetic characteristics of the primary tumor, and maintains the heterogeneity of the patient's tumor. At present, the PDX tumor model is used as a platform for testing drug responses, simulating the metabolic pathways of different drugs in vivo and their therapeutic effects on tumors. The pharmacodynamic results of the PDX tumor model have a high correlation with the clinic, and the pharmacodynamic results are more consistent with the clinical res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 屠志刚刘晗青
Owner 江苏莱森生物科技研究院有限公司
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