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Hatching solution used for hatching mosquito eggs of Aedes albopictus and hatching method of mosquito eggs of Aedes albopictus

A technology of Aedes albopictus and hatching solution, which is applied in animal husbandry and other fields, can solve the problems that are not conducive to large-scale production standardized operations and cost control, cumbersome operation, high energy consumption, etc., and achieve good larval growth synchronization and high hatching rate , the effect of no energy consumption

Active Publication Date: 2017-11-24
GUANGZHOU WOLBAKI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Heating is required during the hatching process, the energy consumption is high, and the operation is cumbersome, and the airtightness of the container is strictly required
Not conducive to standardized operations and cost control for mass production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The present embodiment provides a method for hatching eggs of Aedes albopictus, comprising the following steps:

[0031] Take yeast peptone and add water to prepare an aqueous solution with a concentration of 0.9g / L as the hatching solution;

[0032] Place the hatching liquid at a temperature of 25°C to 30°C to ferment for 24 hours to obtain a fermented hatching liquid;

[0033] Weigh 0.14g of Aedes albopictus eggs into a 50mL incubation tube, and add 33.3mL of fermented hatching liquid into the hatching tube (the fermented hatching liquid accounts for 2 / 3 of the volume of the hatching tube); seal the tube of the hatching tube Cover, shake well, place horizontally, and incubate at a temperature of 25°C to 30°C for 1 hour, then open the cap of the incubation tube, and transfer the hatched larvae to a rearing tray.

Embodiment 2

[0035] The present embodiment provides a method for hatching eggs of Aedes albopictus, comprising the following steps:

[0036] Take yeast peptone and add water to prepare an aqueous solution with a concentration of 0.9g / L as the hatching solution;

[0037] Place the hatching liquid at a temperature of 25°C to 30°C to ferment for 24 hours to obtain a fermented hatching liquid;

[0038] Weigh 0.14g Aedes albopictus ovum and add in the 50mL incubation tube, add 25mL fermented hatching liquid in the hatching tube (the hatching liquid after fermentation accounts for 50% of the hatching tube volume); Seal the tube lid of the hatching tube, Shake well, and incubate at a temperature of 25° C. to 30° C. for 1 hour, then open the cap of the incubation tube, and transfer the hatched larvae to a rearing tray.

Embodiment 3

[0040] The present embodiment provides a method for hatching eggs of Aedes albopictus, comprising the following steps:

[0041] Take yeast peptone and add water to prepare an aqueous solution with a concentration of 0.9g / L as the hatching solution;

[0042] Place the hatching liquid at a temperature of 25°C to 30°C to ferment for 24 hours to obtain a fermented hatching liquid;

[0043] Weigh 0.14g Aedes albopictus ovum and add in the 50mL incubation tube, add 40mL fermented hatching liquid in the hatching tube (the hatching liquid after fermentation accounts for 80% of the hatching tube volume); Seal the tube lid of the hatching tube, Shake well, and incubate at a temperature of 25° C. to 30° C. for 1 hour, then open the cap of the incubation tube, and transfer the hatched larvae to a rearing tray.

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Abstract

The invention relates to a hatching solution used for hatching mosquito eggs of Aedes albopictus and a hatching method of the mosquito eggs of the Aedes albopictus. The hatching solution is an aqueous solution prepared from yeast peptone and water, wherein the concentration of the yeast peptone in the aqueous solution is 0.7-1.5 g / L. The hatching method comprises the following steps that fermentation is conducted on the hatching solution used for hatching the mosquito eggs of the Aedes albopictus; the mosquito eggs of the Aedes albopictus are weighed and put into a hatching pipe, the fermented hatching solution is added into the hatching pipe, a pipe cover of the hatching pipe is sealed, the hatching pipe is evenly shook, standing is conducted on the hatching pipe for hatching, the pipe cover of the hatching pipe is opened, and hatched larvae is transferred into a breeding plate. By means of the hatching solution, the mosquito eggs of the Aedes albopictus are hatched under certain conditions, the hatching speed of the mosquito eggs of the Aedes albopictus is greatly increased, the hatching rate of the active mosquito eggs in one hour is high, and the synchronism of growth of the larvae is good; the hatching method has the advantages that the survival rate of the larvae is high, operation is simple, and the hatching cost is low.

Description

technical field [0001] The invention relates to the technical field of artificial breeding of Aedes albopictus, in particular to a hatching solution for hatching eggs of Aedes albopictus and a method for hatching eggs of Aedes albopictus. Background technique [0002] As an emerging technology in pest control, sterile male insect release technology is used to reduce or even eliminate the insects by cultivating sterile male insects and releasing them to the breeding grounds of pests. After mating with wild female insects, they cannot reproduce offspring. Compared with traditional insecticide deworming strategies, this technology is environmentally friendly and has no impact on other insects and the environment. Mosquitoes serve as intermediate hosts for dengue, Zika, malaria, yellow fever, filariasis, Japanese encephalitis, and other pathogens. In the large-scale Aedes albopictus male mosquito release program, the incubation of mosquito eggs is an important link, so it is ne...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 不公告发明人
Owner GUANGZHOU WOLBAKI BIOTECH CO LTD
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