Test paper plate for detecting solanine in potatoes

Abstract

The invention discloses a test paper plate for detecting solanine in potatoes. The test paper plate comprises a base plate, a lining plate and a protective film, a colloidal gold marking pad, a water-absorption pad, a sample pad and a cellulose acetate membrane are adhered to the lining plate in sequence, the overlapping length of every adjacent part of the colloidal gold marking pad, the water-absorption pad, the sample pad and the cellulose acetate membrane is 0.5-0.8 mm, the colloidal gold marking pad is provided with a monoclonal antibody which is coupled with colloidal gold, the cellulose acetate membrane is provided with a detection line, a quality control line and a false positive marked line, and the sample pad is provided with a filter membrane layer. The test paper plate has the advantages of being high in sensitivity, strong in specificity, low in cost, simple in operation, short in detection time, applicable to units of various kinds, easy to store, and long in shelf date; meanwhile, the operation is simple, fast and safe, a large part of raw materials necessary to the reaction are integrated into a PVC backlining, after a sample is subjected to dripping, an antigen-antibody reaction is quickly conduced on a solid-state membrane, and thus the sample detection time is sharply shortened; in addition, the sample needs no special treatment, and the test paper plate is low in cost, easy to popularize, low in production cost, low in investment and quick in effect.

Description

technical field [0001] The invention relates to a colloidal gold detection reagent board, in particular to a test paper board for detecting solanine in potatoes. Background technique [0002] Potatoes, also known as potatoes and artichokes, are the fourth largest food crops after rice, wheat and corn. Because potato plants and tubers generally contain a class of steroidal glycoside alkaloids, called solanine, also known as solanine (currently known solanine has α, β, γ-solanine or α, β, γ- Chaconine) has certain toxicity, and there is a certain safety hazard in the process of eating potatoes. Potatoes are easy to turn green and germinate during storage, and the content of solanine in the green skin and sprouts will increase rapidly. Excessive intake (about 30g of green potatoes) will cause numbness of the tongue, nausea and vomiting, diarrhea, headache, dizziness dizziness and other adverse reactions, and even lead to convulsions, coma, paralysis of the respiratory center ...

AI Technical Summary

Problems solved by technology

However, this method requires contact with standard solanine, which is not conducive to protecting the health of the operator, and the activity of the enzyme is easily affected by temperature. Therefore, the reagent needs to be stored at low temperature, has a short life, and is likely to cause poor repeatability of the measurement results.

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