Test strip for detecting solanine and application of test strip

A technology of solanine and test strips, which is applied in the field of detection of solanine residues in colloidal gold test strips, eggplants, potatoes and unripe tomatoes

Pending Publication Date: 2022-02-08
ZHEJIANG INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solanin is soluble in water, corrosive and hemolytic, but

Method used

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  • Test strip for detecting solanine and application of test strip
  • Test strip for detecting solanine and application of test strip
  • Test strip for detecting solanine and application of test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of embodiment 1 solanin detection test strip

[0037] The preparation method of this test strip mainly comprises the following steps:

[0038] 1) Prepare a conjugate release pad sprayed with solanine monoclonal antibody-colloidal gold marker;

[0039] 2) Prepare a reaction membrane with a detection line coated with a solanine hapten-carrier protein conjugate and a quality control line coated with a goat anti-mouse anti-antibody;

[0040] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the PVC bottom plate into a test strip.

[0041] The following step-by-step detailed description:

[0042] 1. Preparation of solanin hapten

[0043] Take 0.868g of α-solanine, add 200ml of dry cyclohexane to dissolve, add 0.318g of mercaptopropionic acid, stir well, add peroxide initiator dilauroyl peroxide (LPO) 0.1g, stir at room temperature for 4h, stop the reaction , add 50ml...

Embodiment 2

[0076] The detection of solanine residue in the sample of embodiment 2

[0077] 1. Test with test strips

[0078] Use a straw to absorb the sample solution to be tested and add 3 drops vertically to the sample hole, start timing when the liquid flows, react for 5-10 minutes, and judge the result.

[0079] 2. Analyze test results

[0080] Read the result by colloidal gold analyzer (referred to as "analyzer"):

[0081] Negative (-): Indicates that the concentration of the substance to be tested in the sample is lower than the detection limit;

[0082] Positive (+): Indicates that the concentration of the substance to be tested in the sample is equal to or higher than the detection limit;

[0083] Invalid: Indicates that retesting is required.

Embodiment 3

[0084] Example 3 sample detection example

[0085] 1. Detection limit test

[0086] Take blank potato, eggplant, and immature tomato samples, add solanine to them to the final concentration of 5, 10, and 20 μg / kg, respectively, take test strips for detection, and repeat the measurement three times for each sample.

[0087] When using test strips to detect samples of potatoes, eggplants, and unripe tomatoes, when the concentration of solanine added is 5 μg / kg, the analyzer shows negative; when the concentration of solanine added is 10, 20 μg / kg, the analyzer Shown as positive.

[0088] 2. False positive rate and false negative rate test

[0089] Take 20 positive samples of potatoes, eggplants, and unripe tomatoes with a known solanine content of 10 μg / kg and 20 negative samples of potatoes, eggplants, and unripe tomatoes with a content of less than 5 μg / kg, and use three batches of test strips for detection , to calculate its negative-positive rate. The results are shown in...

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PUM

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Abstract

The invention discloses a test strip for detecting solanine and application of the test strip. The test strip comprises a sample absorption pad (1), a conjugate release pad (2), a reaction film (3), a water absorption pad (4) and a bottom plate (7), the reaction film is provided with a detection line (5) coated with a solanine hapten-carrier protein conjugate and a quality control line (6) coated with a goat anti-mouse antibody, and the conjugate release pad (2) is sprayed with a solanine monoclonal antibody-colloidal gold marker. The invention also provides a method for detecting solanine residues in potatoes, eggplants and immature tomatoes by using the solanine test strip. The test strip provided by the invention has the characteristics of simplicity in operation, high sensitivity, high detection speed, low cost and the like, and is suitable for screening and on-site monitoring of a large number of samples.

Description

technical field [0001] The invention relates to a test strip for detecting solanine and its application, in particular to a colloidal gold test strip for detecting solanin, which is especially suitable for detecting solanin residues in potatoes, eggplants, and unripe tomatoes detection. Background technique [0002] Solanine (Solanine) is a toxic substance in potatoes, also called potato toxin (also found in eggplant, unripe tomatoes), and is a toxic glycoalkaloid. Solanin is soluble in water, corrosive and hemolytic, but it can decompose and destroy when heated with acetic acid. Generally, the solanine contained in every 100 grams of potatoes is only about 10 mg, which will not cause poisoning. However, for immature potatoes or potatoes that turn green and germinate due to exposure to sunlight during storage, the content of solanine per hectogram can be as high as 500 mg. If a large amount of this potato is eaten, it may cause acute poisoning. [0003] The content of sol...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558G01N33/58G01N33/543G01N33/53G01N33/52
CPCG01N33/577G01N33/558G01N33/587G01N33/54346G01N33/5308G01N33/52
Inventor 刘柱王峰黄巧巧赵超群陈碧莲徐潇颖沈泓
Owner ZHEJIANG INST FOR FOOD & DRUG CONTROL
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