Potato stem apex culture medium and preparation method and application thereof

A medium formula and medium technology, applied in the field of regeneration of plant tissue culture technology, can solve the problems of uncertain substances produced by decomposition, destroying the electrolyte balance of medium, slow growth rate of shoot tip culture, etc., so as to improve quality and reduce The effect of raising seedling cycle and improving detoxification efficiency

Inactive Publication Date: 2017-05-31
JIANGXI YIXINTANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the shoot tip culture process, the ammonium nitrogen content of 1 concentration of MS medium is too high, and other nutrients are added, which will slow the growth rate in the shoot tip culture, and the L-cysteine ​

Method used

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  • Potato stem apex culture medium and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of potato shoot tip culture medium, described culture medium formula is: 0.8MS medium, 210mg / L KNO 3 , 65mg / L Ca(H 2 PO 4 ) 2 ·H 2 O, 0.15mg / L solanine, 0.3mg / L indole acetic acid, 0.3mg / L 6-benzyl purine, 0.12mg / L gibberellic acid, 0.7mg / L glutathione, 32g / L sucrose and 9g / L agar.

[0029] The preparation method of described culture medium is, and the steps are:

[0030] S1: Dilute the MS medium to 0.8 times the original concentration, then add KNO 3 , Ca(H 2 PO 4 ) 2 ·H 2 O, solanine, indole acetic acid, 6-benzyl purine, gibberellic acid, glutathione, stirred evenly, and the stirring speed was 50r / min to obtain liquid A;

[0031] S2: Take 1 / 3 of the liquid A prepared in step S1 and pour it into a small aluminum pot to boil, then pour sucrose and agar into the small aluminum pot, and stir while heating at a stirring speed of 50 r / min until the agar is completely melted. Until it becomes clear to the bottom, then pour in the remaining liquid A and stir...

Embodiment 2

[0034] A kind of potato shoot tip culture medium, described culture medium formula is: 0.8MS medium, 150mg / L KNO 3 , 40mg / L Ca(H 2 PO 4 ) 2 ·H 2 O, 0.3mg / L solanine, 0.2mg / L indole acetic acid, 0.5mg / L 6-benzyl purine, 0.05mg / L gibberellic acid, 1mg / L glutathione, 25g / L sucrose and 10g / L agar.

[0035] The preparation method of the medium is similar to Example 1.

Embodiment 3

[0037] A kind of potato shoot tip culture medium, described culture medium formula is: 0.8MS culture medium, 250mg / L KNO 3 , 100mg / L Ca(H 2 PO 4 ) 2 ·H 2 O, 0.1mg / L solanine, 0.4mg / L indole acetic acid, 0.2mg / L 6-benzyl purine, 0.2mg / L gibberellic acid, 0.5mg / L glutathione, 35g / L sucrose and 8g / L agar.

[0038] The preparation method of the medium is similar to Example 1.

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Abstract

The invention belongs to the field of regeneration through a plant tissue culture technology, and particularly relates to a potato stem apex culture medium and a preparation method and application thereof. The potato stem apex culture medium is prepared from a 0.8MS culture medium, 150-250mg/L of KNO3, 40 to 100mg/L of Ca(H2PO4)2.H2O, 0.1 to 0.3mg/L of solanine, 0.2 to 0.4mg/L of indoleacetic acid, 0.2 to 0.5mg/L of 6-benzyl adenine, 0.05 to 0.2mg/L of gibberellic acid, 0.5 to 1mg/L glutathione, 25 to 35g/L of sugar cane sugar and 8 to 10g/L of agar. By adopting the potato stem apex culture medium, nutrient substances of the culture medium are enriched, the quality of potato plants is improved, the growing speed of potatoes is increased, the seedling period is shortened, the cost is lowered, and the detoxifying efficiency is increased.

Description

technical field [0001] The invention belongs to the regeneration field of plant tissue culture technology, and specifically relates to a potato shoot tip culture medium, a preparation method and an application thereof. Background technique [0002] Due to environmental pollution or the accumulation of viruses from generation to generation, a large number of viruses have been deposited in the current plants, causing plant diseases to affect the quality and quantity of products; if it is an edible plant, it will seriously affect people's health. Among them, it belongs to asexual reproduction. Plants are very susceptible to virus infection during the growth process. For example, there are as many as 30 kinds of potato viruses, and as time goes on, more and more types of potato viruses are inherited from generation to generation of plants. Potato virus has now become a global problem, seriously affecting the development of agriculture. [0003] At present, common chemical agent...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 胡波钟勇财谢玉国
Owner JIANGXI YIXINTANG MEDICAL TECH
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