Quality control method of nightshade total alkali preparations

A quality control method, the technology of Solanum nigrum, applied in the direction of measuring device, test sample preparation, instrument, etc., can solve the problems such as the quality control method of Solanum nigrum total alkaloid raw materials and preparations.

Inactive Publication Date: 2008-08-20
刘阳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, there is no research report on the quality control metho

Method used

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  • Quality control method of nightshade total alkali preparations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0189] Example 1. A kind of quality control method of total alkaloid preparation of Solanum nigrum, the identification method of preparation is as follows:

[0190] Take this product, containing 10 mg of total alkaloids of Solanum nigrum, put it in a 2ml measuring bottle, add 2ml of methanol, sonicate for 20 minutes to dissolve, add methanol to the mark, and use it as the test solution; For the reference substance, add methanol to dissolve and dilute it to a solution containing 2mg per 1ml as the reference substance solution;

[0191] According to the thin-layer chromatography test, draw 5 μl of each of the above three solutions, and spot them on the same silica gel G plate with sodium carboxymethylcellulose as the binder, and use chloroform:methanol:water (2:1:0.1) as the Developing agent, unfold about 15cm, take it out, dry it, spray with paraformaldehyde phosphoric acid solution, heat at 105°C until the spot color is clear;

[0192] In the chromatogram of the test product...

Embodiment 2

[0193] Example 2. A kind of quality control method of total alkaloid preparation of Solanum nigrum, the identification method of preparation is as follows:

[0194] Take this product, containing 5 mg of total alkaloids of Solanum nigrum, put it in a 2ml measuring bottle, add 2ml of methanol, sonicate for 20 minutes to dissolve, add methanol to the mark, and use it as the test solution; For the reference substance, add methanol to dissolve and dilute it to a solution containing 2mg per 1ml as the reference substance solution;

[0195] According to the thin-layer chromatography test, draw 5 μl of each of the above three solutions, and spot them on the same silica gel G plate with sodium carboxymethylcellulose as the binder, and use chloroform:methanol:water (3:1.5:0.2) as the Developing agent, unfold about 15cm, take it out, dry it, spray with paraformaldehyde phosphoric acid solution, heat at 105°C until the spot color is clear;

[0196] In the chromatogram of the test produc...

Embodiment 3

[0197] Example 3. A kind of quality control method of total alkaloid preparation of Solanum nigrum, the identification method of preparation is as follows:

[0198] Take this product, containing 15 mg of total alkaloids of Solanum nigrum, put it in a 2ml measuring bottle, add 2ml of methanol, sonicate for 20 minutes to dissolve, add methanol to the mark, and use it as the test solution; For the reference substance, add methanol to dissolve and dilute it to a solution containing 2mg per 1ml as the reference substance solution;

[0199] According to the thin-layer chromatography test, draw 5 μl of each of the above three solutions, and spot them on the same silica gel G plate with sodium carboxymethylcellulose as the binder, and use chloroform:methanol:water (2.5:1..2: 0.15) is the developer, spread about 15cm, take it out, dry it, spray it with phosphoric acid solution of paraformaldehyde, and heat it at 105°C until the color of the spot is clear;

[0200] In the chromatogram...

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Abstract

The invention provides a quality control method of Carina total alkali preparation, whose characteristic resides in that, the identification method of the preparation are as follows: taking the product containing Carina total alkali 5-15 mg, placing it in 2mL volumetric flask, adding 2mL, ultrasonic treating for 20 minutes to make it dissolving, adding methanol to the scale as the solution on approval; then taking solasonine and solamargine as reference substance, adding methanol to dissolve it then diluting into 2mg/mL solution as reference solution; thin layer chromatography, fetching 5 microlitres of each of the three kinds of solutions, marking it respectively into one silica gel G plant with sodium carboxymethyl cellulose as bond, using chloroform: methanol: water (2-3:1-1.5:0.1-0.2) as developing agent, developing to about 15cm, removing, drying, spraying with paraformaldehyde in phosphoric acid, heating up to 105 DEG C till the dot is clear; in the preparation chromatography, the dot with the same color is displayed in the corresponding location of the reference chromatography. The invention can control the quality of the preparation further for improving preparation safety.

Description

technical field [0001] The invention relates to a quality control method of a traditional Chinese medicine extract preparation, in particular to a quality control method of a total alkaloid preparation of Solanum nigrum. Background technique [0002] The total alkaloids of Solanum nigrum are extracted and refined from the dry aerial part of Solanum nigrum L. through any extraction process disclosed in the prior art or conventional traditional Chinese medicine process, preferably according to the application number 200510095455.7 and the name " Extraction process and preparation of total plant alkaloids extract with anti-tumor effect" The total plant alkaloids extract (that is, the total alkaloids extract of Solanum nigrum) extracted by the extraction method disclosed in the Chinese patent application, the total alkaloids of Solanum nigrum The content in the extract is preferably more than 50%. The total alkaloid preparation of Solanum nigrum is made of total alkaloids of So...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N1/28G01N30/02G01N30/00
Inventor 丁岗杨时孟兆青张轶伦李明慧朱华荣陆洁蔡静
Owner 刘阳
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