Recyclable method for extracting and separating effective components of solanum nigrum

A technology of active ingredients and separation method, applied in the field of extraction and separation of active ingredients of Solanum nigrum, can solve the problems of inability to extract and separate, single extraction, etc., and achieve the effects of simple method, good reproducibility and good stability

Active Publication Date: 2019-11-26
JILIN NORMAL UNIV
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  • Claims
  • Application Information

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Problems solved by technology

At present, the extraction of these two substances is single, that is, the solanine an...

Method used

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  • Recyclable method for extracting and separating effective components of solanum nigrum

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Effect test

Embodiment 1

[0030] A recyclable method for extracting and separating effective components of Solanum nigrum, the specific steps of the method are as follows:

[0031] (1), preparation of solanine standard solution: accurately weigh the standard substance (25mg) of solanine and solanine, put into 5mL volumetric flask respectively, dissolve with 3mL ethanol, and be settled to 5mL, A concentration of 5 mg·mL was obtained -1 Australian solanine and Australian solanine standard stock solutions. Then, take 2ml each of the storage solutions of the two single reference substances, put them into the same 10mL volumetric flask, and set the volume to 10mL to obtain a mixed standard solution of solanine.

[0032] (2) Preparation of glucose standard solution: Accurately weigh 10mg of glucose, place it in a 100mL volumetric flask, add 20mL of pure water to dissolve it, set the volume to 100mL, and obtain a concentration of 0.1mg·mL -1 glucose standard stock solution.

[0033] (3), Ultrasonic wall-br...

Embodiment 2

[0039] A recyclable method for extracting and separating effective components of Solanum nigrum, the specific steps of the method are as follows:

[0040] (1), preparation of solanine standard solution: accurately weigh the standard substance (25mg) of solanine and solanine, put into 5mL volumetric flask respectively, dissolve with 3mL ethanol, and be settled to 5mL, A concentration of 5 mg·mL was obtained -1 Australian solanine and Australian solanine standard stock solutions. Then, take 2ml each of the storage solutions of the two single reference substances, put them into the same 10mL volumetric flask, and set the volume to 10mL to obtain a mixed standard solution of solanine.

[0041] (2) Preparation of glucose standard solution: Accurately weigh 10mg of glucose, place it in a 100mL volumetric flask, add 20mL of pure water to dissolve it, set the volume to 100mL, and obtain a concentration of 0.1mg·mL -1 glucose standard stock solution.

[0042] (3), Ultrasonic wall-br...

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Abstract

The invention discloses a recyclable method for extracting and separating effective components of solanum nigrum. According to the method, ultrasonic wall-breaking extraction and an intelligent two aqueous phase extraction method are mainly used to realize simultaneous extraction and separation of solanine and solanum polysaccharides. The method has the steps of preparation of a solanine mixture standard solution and a glucose standard stock solution, ultrasonic wall-breaking extraction and separation through two aqueous phase extraction. The method can simultaneously extract two effective components of solanine and solanum polysaccharides in solanum nigrum leaves, and realize the separation and measurement of solanine and solanum polysaccharides. Extraction and separation reagents in themethod can be recycled. According to the method, high-efficiency liquid phases are used to measure solanine, and solanum polysaccharides are measured by an ultraviolet spectrophotometer. The method has the advantages of simple and stable steps and good reproducibility.

Description

technical field [0001] The invention relates to the technical field of extraction and separation of effective components of Solanum nigrum, specifically a recyclable extraction and separation method of effective components of Solanum nigrum, which can simultaneously extract and separate solanine and polysaccharides from Solanum solanum leaves, And the main extraction and separation reagents can be recycled. Background technique [0002] Solanum nigrum, an annual herbaceous plant of the Solanaceae family, is widely distributed throughout China, and is also distributed in temperate tropical regions of Europe, Asia, and America. The whole plant is used as medicine, which can promote blood circulation and remove blood stasis, clear heat and detoxify, and can also treat headache, toothache, dysentery, tuberculosis, wind-heat in children, urinary tract infection and other diseases. Solanine and Solanum polysaccharide are important active ingredients in Solanum nigrum. [0003] S...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/34G01N30/88G01N30/06G01N21/33
CPCG01N1/286G01N1/34G01N30/88G01N30/06G01N21/33G01N2030/062G01N2030/8809
Inventor 逯洋张桂杰李闯丛飚于萍
Owner JILIN NORMAL UNIV
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