A method for simultaneous extraction of cold-pressed tea cake protein and polysaccharide

A technology of simultaneous extraction and protein extraction, applied in the field of tea cake protein and polysaccharide extraction, can solve the problems of low industrial benefit of cold-pressed tea cake, and achieve the effects of high added value, wide application prospect and simple production process.

Inactive Publication Date: 2014-10-29
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the processing of cold-pressed tea cakes mainly includes direct extraction of tea saponin and polysaccharides. The protein content of about 23% has not been utilized for a long time. The benefits of cold-pressed tea cake processing industry are not obvious, and further technology research and development Improve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The present invention is achieved like this, and its processing step is:

[0019] 1) Drying: Adjust the temperature of the new cold-pressed tea cake in a drying oven to 60°C, and dry it for 4 hours;

[0020] 2) Crushing: the dried tea cakes are pulverized by a pulverizer and passed through a 300-mesh sieve;

[0021] 3) Extraction of alkaline proteins and polysaccharides: use sodium hydroxide solution with a concentration of 0.15mol / L as the extractant, adjust the pH to 8.5, the ratio of solid to liquid is 1:20, the temperature is 80°C, the ultrasonic power is 80W, and the extraction is 30min.

[0022] 4) Centrifugation: control the ultrasonic extraction solution at a speed of 2000r / min, and centrifuge for 10 minutes to separate the basic protein and polysaccharide solution;

[0023] 5) Extraction of acidic polysaccharides: use the residues of basic polysaccharides and proteins as raw materials, use citric acid solution with a concentration of 0.5 mol / L as the extractan...

Embodiment 2

[0030] The present invention is achieved like this, and its processing step is:

[0031] 1) Drying: Adjust the temperature of the new cold-pressed tea cake in a drying oven to 70°C and dry it for 4 hours;

[0032] 2) Crushing: the dried tea cakes are pulverized by a pulverizer and passed through a 300-mesh sieve;

[0033] 3) Extraction of alkaline protein and polysaccharide: Use sodium hydroxide solution with a concentration of 0.2 mol / L as the extractant, adjust the pH to 8.5, the ratio of solid to liquid is 1:20, the temperature is 80°C, the ultrasonic power is 80W, and the extraction is 30 minutes.

[0034] 4) Centrifugation: control the ultrasonic extraction solution at a speed of 3000r / min, and centrifuge for 10 minutes to separate the basic protein and polysaccharide solution;

[0035] 5) Extraction of acidic polysaccharides: use the residues of basic polysaccharides and proteins as raw materials, use citric acid solution with a concentration of 1 mol / L as the extractan...

Embodiment 3

[0042] The present invention is achieved like this, and its processing step is:

[0043] 1) Drying: Adjust the temperature of the new cold-pressed tea cake in a drying oven to 65°C, and dry it for 4 hours;

[0044] 2) Crushing: the dried tea cakes are pulverized by a pulverizer and passed through a 300-mesh sieve;

[0045] 3) Extraction of alkaline protein and polysaccharide: use sodium hydroxide solution with a concentration of 0.18mol / L as the extractant, adjust the pH to 8.5, the ratio of solid to liquid is 1:20, the temperature is 80°C, the ultrasonic power is 80W, and the extraction is 30min.

[0046] 4) Centrifugation: control the ultrasonic extraction solution at a speed of 2500r / min, and centrifuge for 10 minutes to separate the basic protein and polysaccharide solution;

[0047] 5) Extraction of acidic polysaccharides: use the residues of basic polysaccharides and proteins as raw materials, use citric acid solution with a concentration of 0.6 mol / L as the extractant,...

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PUM

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Abstract

The invention relates to a method for synchronously extracting proteins and polysaccharides from cold-pressed tea cakes. The method comprises the following technical steps of: (1) drying and cold-pressing the tea cakes obtained after oil making; (2) crushing the cold-pressed tea cakes, and sieving by a sieve with 300 meshes; (3) extracting and centrifuging by adopting a supersonic wave-sodium hydroxide method to prepare the alkaline proteins and polysaccharides; (4) by taking residues of the extracted alkaline proteins and polysaccharides as raw materials, extracting and centrifuging to prepare acidic polysaccharose by adopting a supersonic wave-citric acid method; (5). centrifuging: to be specific, centrifuging at a high speed, wherein precipitations are the proteins, and a liquid supernatant is the polysaccharose; (6) purifying and drying; (7) detecting; and (8) packaging. The method has the advantages that 1. the synchronous extraction of the protein and the polysaccharide from the cold-pressed tea cakes is realized; 2. the yield of the proteins is increased to the maximum limit through the application of an alkaline process and an acid process; 3. the high additional value processing of the cold-pressed tea cakes is realized; and 4. the method is simple in productive technology and has wide application prospect.

Description

technical field [0001] The invention relates to a method for extracting tea cake protein and polysaccharide, in particular to a method for synchronously extracting cold-pressed tea cake protein and polysaccharide. Background technique [0002] Camellia oil has the reputation of "Oriental olive oil". With the attention of the Ministry of Forestry and the increase in consumer demand for products, my country's tea oil processing has achieved unprecedented development in recent years. Mild, the nutrients are preserved to the maximum, the cake has not been denatured, and the cake can be further processed, making this technology recognized and accepted by processing enterprises. Therefore, the output of cold-pressed tea cake is larger. At present, the processing of cold-pressed tea cakes mainly includes direct extraction of tea saponin and polysaccharides. The protein content of about 23% has not been utilized for a long time. The benefits of cold-pressed tea cake processing indus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C07K1/14
Inventor 涂宗财王辉刘光宪
Owner NANCHANG UNIV
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